| Summary: | As one of the most open and dynamic regions in China, the Guangdong-Hong Kong-Macao Greater Bay Area (GBA) has been urbanizing rapidly in recent decades. The surface water in the GBA also has been suffering from urbanization and intensified human activities. The study aimed to characterize the spatiotemporal patterns and assess the losses and gains of surface water caused by urbanization in the GBA via long time-series remote sensing data, which could support the progress towards sustainable development goals (SDGs) set by the United Nations, especially for measuring SDG 6.6.1 indicator. Firstly, utilizing 4750 continuous Landsat TM/ETM+/OLI images during 1986–2020 and the Google Earth Engine cloud platform, the multiple index water detection rule (MIWDR) was performed to extract surface water extent in the GBA. Secondly, we achieved surface water dynamic type classification based on annual water inundation frequency time-series in the GBA. Finally, the spatial distribution and temporal variation of urbanization-induced water losses and gains were analyzed through a land cover transfer matrix. Results showed that (1) the average minimal and maximal surface water extents of the GBA during 1986–2020 were 2017.62 km<sup>2</sup> and 6129.55 km<sup>2</sup>, respectively. The maximal surface water extent fell rapidly from 7897.96 km<sup>2</sup> in 2001 to 5087.46 km<sup>2</sup> in 2020, with a loss speed of 155.41 km<sup>2</sup> per year (<i>R</i><sup>2</sup> = 0.86). (2) The surface water areas of permanent and dynamic types were 1529.02 km<sup>2</sup> and 2064.99 km<sup>2</sup> during 2000–2020, accounting for 42.54% and 57.46% of all water-related areas, respectively. (3) The surface water extent occupied by impervious land surfaces showed a significant linear downward trend (<i>R</i><sup>2</sup> = 0.98, slope = 36.41 km<sup>2</sup> per year), while the surface water restored from impervious land surfaces denoted a slight growing trend (<i>R</i><sup>2</sup> = 0.86, slope = 0.99 km<sup>2</sup> per year). Our study monitored the long-term changes in the surface water of the GBA, which can provide valuable information for the sustainable development of the GBA urban agglomeration. In addition, the proposed framework can easily be implemented in other similar regions worldwide.
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