Characterization and Biological Performance of Marine Sponge Collagen
Abstract This study characterized the morphological aspects of marine collagen - spongin (SPG) extract from marine sponges, as well as, evaluating its in vitro and in vivo biological performance. Aplysina fulva marine sponge was used for the SPG extraction. It was investigated the physicochemical an...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2022-01-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100327&tlng=en |
| _version_ | 1798014561135099904 |
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| author | Kelly Rossetti Fernandes Julia Risso Parisi Matheus de Almeida Cruz Paulo Roberto Gabbai-Armelin Tiago Akira Tashiro de Araújo Alan de França Santana Ingrid Regina Avanzi Francisca Rayanne Silva-Freitas Alessandra Mussi Ribeiro Giovanna Caroline Aparecida do Vale Carlos Alberto Fortulan Renata Neves Granito Ana Claudia Muniz Renno |
| author_facet | Kelly Rossetti Fernandes Julia Risso Parisi Matheus de Almeida Cruz Paulo Roberto Gabbai-Armelin Tiago Akira Tashiro de Araújo Alan de França Santana Ingrid Regina Avanzi Francisca Rayanne Silva-Freitas Alessandra Mussi Ribeiro Giovanna Caroline Aparecida do Vale Carlos Alberto Fortulan Renata Neves Granito Ana Claudia Muniz Renno |
| author_sort | Kelly Rossetti Fernandes |
| collection | DOAJ |
| description | Abstract This study characterized the morphological aspects of marine collagen - spongin (SPG) extract from marine sponges, as well as, evaluating its in vitro and in vivo biological performance. Aplysina fulva marine sponge was used for the SPG extraction. It was investigated the physicochemical and morphological properties of SPG by using scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and compared to PMMA and bovine collagen. Additionally, the SPG cytotoxicity and its influence on cell proliferation, through in vitro tests. Moreover, the in vivo biological response was investigated using an experimental model of tibial bone defect. The results demonstrated that SPG presented an irregular granular aspect, with a composition of OH, C=O, NH, CN and an amorphous profile. Also, in vitro viability results for the L929 and MC3T3 cell lines cultured with SPG extracts demonstrated normal growth in comparison to controls, except for MC3T3 viability at day 3. For in vivo analysis, using tibial bone defects in rats, SPG treated animals presented an increased rate of material resorption and higher granulation and bone formation deposition in the region of the defect, mainly after 45 days. As a conclusion, SPG was successfully extracted. The in vitro and in vivo studies pointed out that SPG samples produced an increase in L929 and MC3T3 viability and improved the performance in tibial bone defects. It can be concluded that SPG can be used as a bone graft for bone regeneration. |
| first_indexed | 2024-04-11T15:20:31Z |
| format | Article |
| id | doaj.art-a181c5c8d2dd45e9ad6c82ad089faced |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T15:20:31Z |
| publishDate | 2022-01-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-a181c5c8d2dd45e9ad6c82ad089faced2022-12-22T04:16:23ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242022-01-016410.1590/1678-4324-2021200592Characterization and Biological Performance of Marine Sponge CollagenKelly Rossetti Fernandeshttps://orcid.org/0000-0003-2851-6896Julia Risso Parisihttps://orcid.org/0000-0002-4325-0638Matheus de Almeida Cruzhttps://orcid.org/0000-0002-9032-9111Paulo Roberto Gabbai-Armelinhttps://orcid.org/0000-0003-4802-0062Tiago Akira Tashiro de Araújohttps://orcid.org/0000-0003-2960-8186Alan de França Santanahttps://orcid.org/0000-0001-8213-8262Ingrid Regina Avanzihttps://orcid.org/0000-0001-5948-1484Francisca Rayanne Silva-Freitashttps://orcid.org/0000-0001-8690-3881Alessandra Mussi Ribeirohttps://orcid.org/0000-0002-7697-5766Giovanna Caroline Aparecida do Valehttps://orcid.org/0000-0002-5375-1241Carlos Alberto Fortulanhttps://orcid.org/0000-0002-2259-9910Renata Neves Granitohttps://orcid.org/0000-0002-2094-3845Ana Claudia Muniz Rennohttps://orcid.org/0000-0003-2358-0514Abstract This study characterized the morphological aspects of marine collagen - spongin (SPG) extract from marine sponges, as well as, evaluating its in vitro and in vivo biological performance. Aplysina fulva marine sponge was used for the SPG extraction. It was investigated the physicochemical and morphological properties of SPG by using scanning electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction and compared to PMMA and bovine collagen. Additionally, the SPG cytotoxicity and its influence on cell proliferation, through in vitro tests. Moreover, the in vivo biological response was investigated using an experimental model of tibial bone defect. The results demonstrated that SPG presented an irregular granular aspect, with a composition of OH, C=O, NH, CN and an amorphous profile. Also, in vitro viability results for the L929 and MC3T3 cell lines cultured with SPG extracts demonstrated normal growth in comparison to controls, except for MC3T3 viability at day 3. For in vivo analysis, using tibial bone defects in rats, SPG treated animals presented an increased rate of material resorption and higher granulation and bone formation deposition in the region of the defect, mainly after 45 days. As a conclusion, SPG was successfully extracted. The in vitro and in vivo studies pointed out that SPG samples produced an increase in L929 and MC3T3 viability and improved the performance in tibial bone defects. It can be concluded that SPG can be used as a bone graft for bone regeneration.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100327&tlng=ensponginAplysina fulvatibial bone defectsbone regenerationbiocompatibility |
| spellingShingle | Kelly Rossetti Fernandes Julia Risso Parisi Matheus de Almeida Cruz Paulo Roberto Gabbai-Armelin Tiago Akira Tashiro de Araújo Alan de França Santana Ingrid Regina Avanzi Francisca Rayanne Silva-Freitas Alessandra Mussi Ribeiro Giovanna Caroline Aparecida do Vale Carlos Alberto Fortulan Renata Neves Granito Ana Claudia Muniz Renno Characterization and Biological Performance of Marine Sponge Collagen Brazilian Archives of Biology and Technology spongin Aplysina fulva tibial bone defects bone regeneration biocompatibility |
| title | Characterization and Biological Performance of Marine Sponge Collagen |
| title_full | Characterization and Biological Performance of Marine Sponge Collagen |
| title_fullStr | Characterization and Biological Performance of Marine Sponge Collagen |
| title_full_unstemmed | Characterization and Biological Performance of Marine Sponge Collagen |
| title_short | Characterization and Biological Performance of Marine Sponge Collagen |
| title_sort | characterization and biological performance of marine sponge collagen |
| topic | spongin Aplysina fulva tibial bone defects bone regeneration biocompatibility |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132021000100327&tlng=en |
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