Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features
Literature reports about Norovirus outbreaks, especially in hospitals, have accumulated in the past years, including patients and health workers.This report describes a small outbreak by Norovirus which occurred in USC Urology with unusual clinical-epidemiological features.The clinical course was un...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2008-12-01
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| Series: | Microbiologia Medica |
| Subjects: | |
| Online Access: | http://www.pagepressjournals.org/index.php/mm/article/view/2562 |
| _version_ | 1818206528422805504 |
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| author | Marco Arosio Annalisa Grigis Enrico Castellucci Giancarla Caglioni Franca Averara Francesco Locati Barbara Marziali Ilaria Di Bartolo Franco Maria Ruggeri Antonio Goglio |
| author_facet | Marco Arosio Annalisa Grigis Enrico Castellucci Giancarla Caglioni Franca Averara Francesco Locati Barbara Marziali Ilaria Di Bartolo Franco Maria Ruggeri Antonio Goglio |
| author_sort | Marco Arosio |
| collection | DOAJ |
| description | Literature reports about Norovirus outbreaks, especially in hospitals, have accumulated in the past years, including patients and health workers.This report describes a small outbreak by Norovirus which occurred in USC Urology with unusual clinical-epidemiological features.The clinical course was unusual, as it presented no vomiting and duration of diarrhoea was unusually long (4-6 days) with the lack of involvement of health workers.The search for the virus was done with immunoassay method RIDASCREEN 2a generation on 11/19 patients (58%) with gastroenteritis symptoms, and 3/11 samples (27%) were positive. The molecular investigation by RT/PCR with diagnostic primers JV12 and JV13 on the polymerase region, carried out at the Istituto Superiore di Sanità, Rome, gave negative results while the RT/PCR using primers GIISKR and GIISKF, which amplify a fragment of the capsid region, was positive in 4/11 samples (36%) showing a correlation of 2/4 samples (50%) with RIDASCREEN immunoassay test. Our observations confirm the usefulness of immunoassay tests and the need to extend the molecular methods using different diagnostic primers. In outbreaks of gastroenteritis the microbiologist should consider the detection of Norovirus in diagnostic routine, as a positive result requires prevention measures to avoid also the spreading by droplets when vomiting is present. |
| first_indexed | 2024-12-12T04:14:28Z |
| format | Article |
| id | doaj.art-a183116f672349889e5d86d0cd1c468a |
| institution | Directory Open Access Journal |
| issn | 2280-6423 |
| language | English |
| last_indexed | 2024-12-12T04:14:28Z |
| publishDate | 2008-12-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Microbiologia Medica |
| spelling | doaj.art-a183116f672349889e5d86d0cd1c468a2022-12-22T00:38:30ZengPAGEPress PublicationsMicrobiologia Medica2280-64232008-12-0123410.4081/mm.2008.25621815Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological featuresMarco ArosioAnnalisa GrigisEnrico CastellucciGiancarla CaglioniFranca AveraraFrancesco LocatiBarbara MarzialiIlaria Di BartoloFranco Maria RuggeriAntonio GoglioLiterature reports about Norovirus outbreaks, especially in hospitals, have accumulated in the past years, including patients and health workers.This report describes a small outbreak by Norovirus which occurred in USC Urology with unusual clinical-epidemiological features.The clinical course was unusual, as it presented no vomiting and duration of diarrhoea was unusually long (4-6 days) with the lack of involvement of health workers.The search for the virus was done with immunoassay method RIDASCREEN 2a generation on 11/19 patients (58%) with gastroenteritis symptoms, and 3/11 samples (27%) were positive. The molecular investigation by RT/PCR with diagnostic primers JV12 and JV13 on the polymerase region, carried out at the Istituto Superiore di Sanità, Rome, gave negative results while the RT/PCR using primers GIISKR and GIISKF, which amplify a fragment of the capsid region, was positive in 4/11 samples (36%) showing a correlation of 2/4 samples (50%) with RIDASCREEN immunoassay test. Our observations confirm the usefulness of immunoassay tests and the need to extend the molecular methods using different diagnostic primers. In outbreaks of gastroenteritis the microbiologist should consider the detection of Norovirus in diagnostic routine, as a positive result requires prevention measures to avoid also the spreading by droplets when vomiting is present.http://www.pagepressjournals.org/index.php/mm/article/view/2562Norovirus, Gastroenteritis, Nosocomial outbreak. |
| spellingShingle | Marco Arosio Annalisa Grigis Enrico Castellucci Giancarla Caglioni Franca Averara Francesco Locati Barbara Marziali Ilaria Di Bartolo Franco Maria Ruggeri Antonio Goglio Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features Microbiologia Medica Norovirus, Gastroenteritis, Nosocomial outbreak. |
| title | Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features |
| title_full | Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features |
| title_fullStr | Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features |
| title_full_unstemmed | Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features |
| title_short | Nosocomial outbreak of norovirus gastroenteritidis with unusual clinical-epidemiological features |
| title_sort | nosocomial outbreak of norovirus gastroenteritidis with unusual clinical epidemiological features |
| topic | Norovirus, Gastroenteritis, Nosocomial outbreak. |
| url | http://www.pagepressjournals.org/index.php/mm/article/view/2562 |
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