Proton and alpha radiation-induced mutational profiles in human cells
Abstract Ionizing radiation is known to be DNA damaging and mutagenic, however less is known about which mutational footprints result from exposures of human cells to different types of radiation. We were interested in the mutagenic effects of particle radiation exposures on genomes of various human...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-36845-3 |
| _version_ | 1797801456198221824 |
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| author | Tiffany M. Delhomme Maia Munteanu Manuela Buonanno Veljko Grilj Josep Biayna Fran Supek |
| author_facet | Tiffany M. Delhomme Maia Munteanu Manuela Buonanno Veljko Grilj Josep Biayna Fran Supek |
| author_sort | Tiffany M. Delhomme |
| collection | DOAJ |
| description | Abstract Ionizing radiation is known to be DNA damaging and mutagenic, however less is known about which mutational footprints result from exposures of human cells to different types of radiation. We were interested in the mutagenic effects of particle radiation exposures on genomes of various human cell types, in order to gauge the genotoxic risks of galactic cosmic radiation, and of certain types of tumor radiotherapy. To this end, we exposed cultured cell lines from the human blood, breast and lung to fractionated proton and alpha particle (helium nuclei) beams at doses sufficient to considerably affect cell viability. Whole-genome sequencing revealed that mutation rates were not overall markedly increased upon proton and alpha exposures. However, there were modest changes in mutation spectra and distributions, such as the increases in clustered mutations and of certain types of indels and structural variants. The spectrum of mutagenic effects of particle beams may be cell-type and/or genetic background specific. Overall, the mutational effects of repeated exposures to proton and alpha radiation on human cells in culture appear subtle, however further work is warranted to understand effects of long-term exposures on various human tissues. |
| first_indexed | 2024-03-13T04:50:44Z |
| format | Article |
| id | doaj.art-a1846ecdc04a47d6a298d2a4c0eaa79b |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-13T04:50:44Z |
| publishDate | 2023-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a1846ecdc04a47d6a298d2a4c0eaa79b2023-06-18T11:14:02ZengNature PortfolioScientific Reports2045-23222023-06-0113111910.1038/s41598-023-36845-3Proton and alpha radiation-induced mutational profiles in human cellsTiffany M. Delhomme0Maia Munteanu1Manuela Buonanno2Veljko Grilj3Josep Biayna4Fran Supek5Genome Data Science, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)Genome Data Science, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)Radiological Research Accelerator Facility (RARAF), Columbia UniversityRadiological Research Accelerator Facility (RARAF), Columbia UniversityGenome Data Science, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)Genome Data Science, Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST)Abstract Ionizing radiation is known to be DNA damaging and mutagenic, however less is known about which mutational footprints result from exposures of human cells to different types of radiation. We were interested in the mutagenic effects of particle radiation exposures on genomes of various human cell types, in order to gauge the genotoxic risks of galactic cosmic radiation, and of certain types of tumor radiotherapy. To this end, we exposed cultured cell lines from the human blood, breast and lung to fractionated proton and alpha particle (helium nuclei) beams at doses sufficient to considerably affect cell viability. Whole-genome sequencing revealed that mutation rates were not overall markedly increased upon proton and alpha exposures. However, there were modest changes in mutation spectra and distributions, such as the increases in clustered mutations and of certain types of indels and structural variants. The spectrum of mutagenic effects of particle beams may be cell-type and/or genetic background specific. Overall, the mutational effects of repeated exposures to proton and alpha radiation on human cells in culture appear subtle, however further work is warranted to understand effects of long-term exposures on various human tissues.https://doi.org/10.1038/s41598-023-36845-3 |
| spellingShingle | Tiffany M. Delhomme Maia Munteanu Manuela Buonanno Veljko Grilj Josep Biayna Fran Supek Proton and alpha radiation-induced mutational profiles in human cells Scientific Reports |
| title | Proton and alpha radiation-induced mutational profiles in human cells |
| title_full | Proton and alpha radiation-induced mutational profiles in human cells |
| title_fullStr | Proton and alpha radiation-induced mutational profiles in human cells |
| title_full_unstemmed | Proton and alpha radiation-induced mutational profiles in human cells |
| title_short | Proton and alpha radiation-induced mutational profiles in human cells |
| title_sort | proton and alpha radiation induced mutational profiles in human cells |
| url | https://doi.org/10.1038/s41598-023-36845-3 |
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