The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD
BackgroundAlthough studies suggest a deficiency in stem cell numbers in chronic airway diseases such as chronic obstructive pulmonary disease (COPD), the role of bronchial epithelial progenitor/stem (P/S) cells is not clear. The objectives of this study were to investigate expression of progenitor/s...
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Frontiers Media S.A.
2023-10-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2023.1118715/full |
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| author | Nuray Bostancieri Nuray Bostancieri Kemal Bakir Seval Kul Ayhan Eralp Ozgecan Kayalar Nur Konyalilar Hadi Rajabi Mehmet Yuncu Ali Önder Yildirim Ali Önder Yildirim Hasan Bayram Hasan Bayram |
| author_facet | Nuray Bostancieri Nuray Bostancieri Kemal Bakir Seval Kul Ayhan Eralp Ozgecan Kayalar Nur Konyalilar Hadi Rajabi Mehmet Yuncu Ali Önder Yildirim Ali Önder Yildirim Hasan Bayram Hasan Bayram |
| author_sort | Nuray Bostancieri |
| collection | DOAJ |
| description | BackgroundAlthough studies suggest a deficiency in stem cell numbers in chronic airway diseases such as chronic obstructive pulmonary disease (COPD), the role of bronchial epithelial progenitor/stem (P/S) cells is not clear. The objectives of this study were to investigate expression of progenitor/stem (P/S) cell markers, cytokeratin (CK) 5, CK14 and p63 in bronchial epithelial explants and cell cultures obtained from smokers with and without COPD following multiple outgrowths, and to study this effect on bronchial epithelial cell (BEC) proliferation.MethodsBronchial epithelial explants were dissected from lung explants and cultured on coverslips. Confluent cultures were obtained after 3–4 weeks’ (transfer, Tr1), explants were then transferred and cultured for a second (Tr2) and third (Tr3) time, respectively. At each stage, expression of CK5, CK14 and p63 in explants and BEC were determined by immunostaining. In parallel experiments, outgrowing cells from explants were counted after 4wks, and explants subsequently transferred to obtain new cultures for a further 3 times.ResultsAs the transfer number advanced, CK5, CK14 and p63 expression was decreased in both explants and BEC from both smokers without COPD and patients with COPD, with a more pronounced decrease in BEC numbers in the COPD group. Total cell numbers cultured from explants were decreased with advancing outgrowth number in both groups. Smoking status and lung function parameters were correlated with reduced P/S marker expression and cell numbers.ConclusionOur findings suggest that the number of P/S cells in airway epithelium may play a role in the pathogenesis of COPD, as well as a role in the proliferation of airway epithelial cells, in vitro. |
| first_indexed | 2024-03-11T18:10:22Z |
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| id | doaj.art-a1847a293bf4468b99ddcd7dd4eb126f |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-03-11T18:10:22Z |
| publishDate | 2023-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Medicine |
| spelling | doaj.art-a1847a293bf4468b99ddcd7dd4eb126f2023-10-16T15:23:14ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2023-10-011010.3389/fmed.2023.11187151118715The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPDNuray Bostancieri0Nuray Bostancieri1Kemal Bakir2Seval Kul3Ayhan Eralp4Ozgecan Kayalar5Nur Konyalilar6Hadi Rajabi7Mehmet Yuncu8Ali Önder Yildirim9Ali Önder Yildirim10Hasan Bayram11Hasan Bayram12Department of Histology and Embryology, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeCell Culture Laboratory, Department of Chest Diseases, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeDepartment of Pathology, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeDepartment of Biostatistics, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeDepartment of Histology and Embryology, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeKoc University Research Center for Translational Medicine, Koc University, Istanbul, TürkiyeKoc University Research Center for Translational Medicine, Koc University, Istanbul, TürkiyeKoc University Research Center for Translational Medicine, Koc University, Istanbul, TürkiyeDepartment of Histology and Embryology, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeKoc University Research Center for Translational Medicine, Koc University, Istanbul, TürkiyeComprehensive Pneumology Center (CPC), Institute of Lung Health and Immunity (LHI), Member of the German Center for Lung Research (DZL), Helmholtz Munich, Munich, GermanyCell Culture Laboratory, Department of Chest Diseases, School of Medicine, University of Gaziantep, Gaziantep, TürkiyeKoc University Research Center for Translational Medicine, Koc University, Istanbul, TürkiyeBackgroundAlthough studies suggest a deficiency in stem cell numbers in chronic airway diseases such as chronic obstructive pulmonary disease (COPD), the role of bronchial epithelial progenitor/stem (P/S) cells is not clear. The objectives of this study were to investigate expression of progenitor/stem (P/S) cell markers, cytokeratin (CK) 5, CK14 and p63 in bronchial epithelial explants and cell cultures obtained from smokers with and without COPD following multiple outgrowths, and to study this effect on bronchial epithelial cell (BEC) proliferation.MethodsBronchial epithelial explants were dissected from lung explants and cultured on coverslips. Confluent cultures were obtained after 3–4 weeks’ (transfer, Tr1), explants were then transferred and cultured for a second (Tr2) and third (Tr3) time, respectively. At each stage, expression of CK5, CK14 and p63 in explants and BEC were determined by immunostaining. In parallel experiments, outgrowing cells from explants were counted after 4wks, and explants subsequently transferred to obtain new cultures for a further 3 times.ResultsAs the transfer number advanced, CK5, CK14 and p63 expression was decreased in both explants and BEC from both smokers without COPD and patients with COPD, with a more pronounced decrease in BEC numbers in the COPD group. Total cell numbers cultured from explants were decreased with advancing outgrowth number in both groups. Smoking status and lung function parameters were correlated with reduced P/S marker expression and cell numbers.ConclusionOur findings suggest that the number of P/S cells in airway epithelium may play a role in the pathogenesis of COPD, as well as a role in the proliferation of airway epithelial cells, in vitro.https://www.frontiersin.org/articles/10.3389/fmed.2023.1118715/fullCOPDsmokerprogenitor/stem cellprimary bronchial epithelial cell culturecytokeratin 5cytokeratin 14 |
| spellingShingle | Nuray Bostancieri Nuray Bostancieri Kemal Bakir Seval Kul Ayhan Eralp Ozgecan Kayalar Nur Konyalilar Hadi Rajabi Mehmet Yuncu Ali Önder Yildirim Ali Önder Yildirim Hasan Bayram Hasan Bayram The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD Frontiers in Medicine COPD smoker progenitor/stem cell primary bronchial epithelial cell culture cytokeratin 5 cytokeratin 14 |
| title | The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD |
| title_full | The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD |
| title_fullStr | The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD |
| title_full_unstemmed | The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD |
| title_short | The effect of multiple outgrowths from bronchial tissue explants on progenitor/stem cell number in primary bronchial epithelial cell cultures from smokers and patients with COPD |
| title_sort | effect of multiple outgrowths from bronchial tissue explants on progenitor stem cell number in primary bronchial epithelial cell cultures from smokers and patients with copd |
| topic | COPD smoker progenitor/stem cell primary bronchial epithelial cell culture cytokeratin 5 cytokeratin 14 |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2023.1118715/full |
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