Gallic acid regulates immune response in a mouse model of rheumatoid arthritis
Abstract Introduction Previous studies revealed that gallic acid (GA) exerts anti‐inflammation and immuno‐regulatory properties. This study aims to explore the pharmacological activities of GA in collagen‐induced arthritis (CIA) mouse model. Methods Male DBA/1J mice were used to construct the CIA mo...
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| Format: | Article |
| Language: | English |
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Wiley
2023-02-01
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| Series: | Immunity, Inflammation and Disease |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/iid3.782 |
| _version_ | 1797816615599865856 |
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| author | Siqin Liu Jiajia Li Le‐heng Feng |
| author_facet | Siqin Liu Jiajia Li Le‐heng Feng |
| author_sort | Siqin Liu |
| collection | DOAJ |
| description | Abstract Introduction Previous studies revealed that gallic acid (GA) exerts anti‐inflammation and immuno‐regulatory properties. This study aims to explore the pharmacological activities of GA in collagen‐induced arthritis (CIA) mouse model. Methods Male DBA/1J mice were used to construct the CIA model. The mice were administrated with GA for 3 weeks. Clinical arthritis scores and hind paw volume were evaluated over the experimental period. qPCR and Western blot analysis were used to determine the levels of matrix metallopeptidases (MMPs) and cytokines. In addition, flow cytometry was used to measure the populations of Th17 and Treg cells. ELISAs were used to determine the cytokines in the serum and ankle joint tissues. Results Treatment of GA (40 and 80 mg/kg/d) reduced clinical arthritis scores and hind paw volume in the CIA mouse model. Besides, treatment of GA reduced the overexpression of MMPs and modulated the dysregulation of inflammation‐related cytokines. Flow cytometry showed that treatment of GA decreased the population of Th17 cells, and increased the population of Treg cells, as supported by treatment of GA regulated the Th17/Treg‐related cytokines. Conclusions GA attenuates symptoms in the CIA mouse model by anti‐inflammation and regulating Th17/Treg cell imbalance. |
| first_indexed | 2024-03-13T08:41:16Z |
| format | Article |
| id | doaj.art-a185d8f2624a4d88871e975d45417572 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-03-13T08:41:16Z |
| publishDate | 2023-02-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-a185d8f2624a4d88871e975d454175722023-05-30T12:15:46ZengWileyImmunity, Inflammation and Disease2050-45272023-02-01112n/an/a10.1002/iid3.782Gallic acid regulates immune response in a mouse model of rheumatoid arthritisSiqin Liu0Jiajia Li1Le‐heng Feng2Department of Laboratory Daqing Oilfield General Hospital Daqing Heilongjiang ChinaDepartment of Laboratory Daqing Oilfield General Hospital Daqing Heilongjiang ChinaDepartment of Rheumatism, The First Hospital of Qiqihar Affiliated Qiqihar Hospital of Southern Medical University Qiqihar Heilongjiang ChinaAbstract Introduction Previous studies revealed that gallic acid (GA) exerts anti‐inflammation and immuno‐regulatory properties. This study aims to explore the pharmacological activities of GA in collagen‐induced arthritis (CIA) mouse model. Methods Male DBA/1J mice were used to construct the CIA model. The mice were administrated with GA for 3 weeks. Clinical arthritis scores and hind paw volume were evaluated over the experimental period. qPCR and Western blot analysis were used to determine the levels of matrix metallopeptidases (MMPs) and cytokines. In addition, flow cytometry was used to measure the populations of Th17 and Treg cells. ELISAs were used to determine the cytokines in the serum and ankle joint tissues. Results Treatment of GA (40 and 80 mg/kg/d) reduced clinical arthritis scores and hind paw volume in the CIA mouse model. Besides, treatment of GA reduced the overexpression of MMPs and modulated the dysregulation of inflammation‐related cytokines. Flow cytometry showed that treatment of GA decreased the population of Th17 cells, and increased the population of Treg cells, as supported by treatment of GA regulated the Th17/Treg‐related cytokines. Conclusions GA attenuates symptoms in the CIA mouse model by anti‐inflammation and regulating Th17/Treg cell imbalance.https://doi.org/10.1002/iid3.782collagen‐induced arthritisgallic acidinflammationTh17Treg |
| spellingShingle | Siqin Liu Jiajia Li Le‐heng Feng Gallic acid regulates immune response in a mouse model of rheumatoid arthritis Immunity, Inflammation and Disease collagen‐induced arthritis gallic acid inflammation Th17 Treg |
| title | Gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| title_full | Gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| title_fullStr | Gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| title_full_unstemmed | Gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| title_short | Gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| title_sort | gallic acid regulates immune response in a mouse model of rheumatoid arthritis |
| topic | collagen‐induced arthritis gallic acid inflammation Th17 Treg |
| url | https://doi.org/10.1002/iid3.782 |
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