Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of <i>Aspergillus</i> species in the Human Airway

Evaluation of Droplet Digital Polymerase Chain Reaction (ddPCR) for the Absolute Quantification of <i>Aspergillus</i> species in the Human Airway

Background: Prior studies illustrate the presence and clinical importance of detecting <i>Aspergillus</i> species in the airways of patients with chronic respiratory disease. Despite this, a low fungal biomass and the presence of PCR inhibitors limits the usefulness of quantitative PCR (...

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Bibliographic Details
Main Authors: Tuang Yeow Poh, Nur A’tikah Binte Mohamed Ali, Louisa L.Y. Chan, Pei Yee Tiew, Sanjay H. Chotirmall
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:International Journal of Molecular Sciences
Subjects:
<i>Aspergillus fumigatus</i>
<i>Aspergillus terreus</i>
qPCR
ddPCR
quantification
Online Access:https://www.mdpi.com/1422-0067/21/9/3043

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