Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa
Objectives: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data. Methods: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971223005210 |
| _version_ | 1797810583500750848 |
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| author | Philippe Selhorst Sebastian Lequime Gytis Dudas Sam Proesmans Pascal Lutumba Freddy Katshongo Kadrie Ramadan Isabel Micalessi Steve Ahuka-Mundeke Veerle Vanlerberghe Marjan Van Esbroeck Kevin K. Ariën |
| author_facet | Philippe Selhorst Sebastian Lequime Gytis Dudas Sam Proesmans Pascal Lutumba Freddy Katshongo Kadrie Ramadan Isabel Micalessi Steve Ahuka-Mundeke Veerle Vanlerberghe Marjan Van Esbroeck Kevin K. Ariën |
| author_sort | Philippe Selhorst |
| collection | DOAJ |
| description | Objectives: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data. Methods: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences. Results: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study. Conclusion: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden. |
| first_indexed | 2024-03-13T07:11:04Z |
| format | Article |
| id | doaj.art-a1867296178b48909709f67fe90228e6 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-03-13T07:11:04Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-a1867296178b48909709f67fe90228e62023-06-06T04:00:51ZengElsevierInternational Journal of Infectious Diseases1201-97122023-08-011334652Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in AfricaPhilippe Selhorst0Sebastian Lequime1Gytis Dudas2Sam Proesmans3Pascal Lutumba4Freddy Katshongo5Kadrie Ramadan6Isabel Micalessi7Steve Ahuka-Mundeke8Veerle Vanlerberghe9Marjan Van Esbroeck10Kevin K. Ariën11Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; The Outbreak Research Team, Institute of Tropical Medicine, Antwerp, Belgium; Corresponding author: Tel: +32(0)32476546.Microbial Ecology Cluster, Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, The NetherlandsInstitute of Biotechnology, Life Sciences Centre, Vilnius University, Vilnius, LithuaniaDepartment of Internal Medicine, Infectious Diseases & Tropical Medicine, University of Antwerp, Antwerp, BelgiumNational Institute of Biomedical Research, Kinshasa, Democratic Republic of Congo; University of Kinshasa, Kinshasa, Democratic Republic of CongoHigher Institute of Medical Techniques, Kinshasa, Democratic Republic of CongoDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumNational Institute of Biomedical Research, Kinshasa, Democratic Republic of Congo; Biomedical Department, Kinshasa Teaching Hospital, School of Medicine, Kinshasa University, Kinshasa, Democratic Republic of CongoDepartment of Public Health, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDepartment of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; Departement Biomedische Wetenschappen, University of Antwerp, Antwerp, BelgiumObjectives: The origin and spread of dengue virus (DENV) circulating in Africa remain poorly characterized, with African sequences representing <1% of global sequence data. Methods: Whole genome sequencing was performed on serum samples (n = 29) from an undifferentiated fever study in 2016 in the Democratic Republic of Congo (DRC), and from febrile travelers returning from Africa. The evolutionary history of the newly acquired African DENV-1 (n = 1) and cosmopolitan genotype DENV-2 (n = 18) genomes was reconstructed using a phylogeographic, time-scaled Bayesian analysis on a curated DENV panel including all known African sequences. Results: A minimum of 10 and eight introductions could be identified into Africa for DENV-1 and cosmopolitan DENV-2, respectively, almost all originating from Asia. Three introductions were previously unknown. The currently circulating virus comprises mainly the recently introduced clades and one long-established African clade. Robust geographical clustering suggests limited spread of DENV after each introduction. Our data identified the DRC as the source of the 2018 Angolan DENV-2 epidemic, and similarly, the 2013 Angolan DENV-1 outbreak as the origin of our DRC study. Conclusion: Active genomic surveillance of DENV in Africa at the portals of entry might help early outbreak response and limit sero- and genotype spread and human disease burden.http://www.sciencedirect.com/science/article/pii/S1201971223005210DengueDENVPhylogeneticsAfricaWhole genome sequencing |
| spellingShingle | Philippe Selhorst Sebastian Lequime Gytis Dudas Sam Proesmans Pascal Lutumba Freddy Katshongo Kadrie Ramadan Isabel Micalessi Steve Ahuka-Mundeke Veerle Vanlerberghe Marjan Van Esbroeck Kevin K. Ariën Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa International Journal of Infectious Diseases Dengue DENV Phylogenetics Africa Whole genome sequencing |
| title | Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa |
| title_full | Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa |
| title_fullStr | Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa |
| title_full_unstemmed | Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa |
| title_short | Phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in Africa |
| title_sort | phylogeographic analysis of dengue virus serotype 1 and cosmopolitan serotype 2 in africa |
| topic | Dengue DENV Phylogenetics Africa Whole genome sequencing |
| url | http://www.sciencedirect.com/science/article/pii/S1201971223005210 |
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