Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency
Boron (B) deficiency stress is frequently observed in pear orchards and causes a considerable loss of productivity and fruit quality. <i>Pyrus betulaefolia</i> is one of the most important rootstocks that has been widely used in pear production. The present study confirmed that the boron...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/14/4/817 |
| _version_ | 1797605312005406720 |
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| author | Jing Liu Tao Chen Chun-Lei Wang Xiao Liu |
| author_facet | Jing Liu Tao Chen Chun-Lei Wang Xiao Liu |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | Boron (B) deficiency stress is frequently observed in pear orchards and causes a considerable loss of productivity and fruit quality. <i>Pyrus betulaefolia</i> is one of the most important rootstocks that has been widely used in pear production. The present study confirmed that the boron form of different tissues showed various changes, and the free boron content was significantly decreased under the short-term B deficiency condition. Moreover, the ABA and JA content also significantly accumulated in the root after short-term B deficiency treatment. A comprehensive transcriptome analysis of 24 h B deficiency treatment <i>P. betulaefolia</i> root was performed in this study. Transcriptome results revealed a total of 1230 up-regulated and 642 down-regulated differentially expressed genes (DEGs), respectively. B deficiency significantly increased the expression of the key aquaporin gene NIP5-1. In addition, B deficiency also increased the expression of ABA (ZEP and NCED) and JA (LOX, AOS and OPR) synthesis genes. Several MYB, WRKY, bHLH and ERF transcription factors were induced by B deficiency stress, which may relate to the regulation of B uptake and plant hormone synthesis. Overall, these findings suggested that <i>P. betulaefolia</i> root had adaptive responses to short-term B deficiency stress by improved boron absorption ability and hormone (JA and ABA) synthesis. The transcriptome analysis provided further information for understanding the mechanism of the pear rootstock responses to B deficiency stress. |
| first_indexed | 2024-03-11T05:00:18Z |
| format | Article |
| id | doaj.art-a189c44d49244ec688b3523813154bfe |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-11T05:00:18Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-a189c44d49244ec688b3523813154bfe2023-11-17T19:22:53ZengMDPI AGGenes2073-44252023-03-0114481710.3390/genes14040817Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron DeficiencyJing Liu0Tao Chen1Chun-Lei Wang2Xiao Liu3College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaCollege of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, ChinaBoron (B) deficiency stress is frequently observed in pear orchards and causes a considerable loss of productivity and fruit quality. <i>Pyrus betulaefolia</i> is one of the most important rootstocks that has been widely used in pear production. The present study confirmed that the boron form of different tissues showed various changes, and the free boron content was significantly decreased under the short-term B deficiency condition. Moreover, the ABA and JA content also significantly accumulated in the root after short-term B deficiency treatment. A comprehensive transcriptome analysis of 24 h B deficiency treatment <i>P. betulaefolia</i> root was performed in this study. Transcriptome results revealed a total of 1230 up-regulated and 642 down-regulated differentially expressed genes (DEGs), respectively. B deficiency significantly increased the expression of the key aquaporin gene NIP5-1. In addition, B deficiency also increased the expression of ABA (ZEP and NCED) and JA (LOX, AOS and OPR) synthesis genes. Several MYB, WRKY, bHLH and ERF transcription factors were induced by B deficiency stress, which may relate to the regulation of B uptake and plant hormone synthesis. Overall, these findings suggested that <i>P. betulaefolia</i> root had adaptive responses to short-term B deficiency stress by improved boron absorption ability and hormone (JA and ABA) synthesis. The transcriptome analysis provided further information for understanding the mechanism of the pear rootstock responses to B deficiency stress.https://www.mdpi.com/2073-4425/14/4/817pear rootstockboron deficiencyaquaporinplant hormonetranscriptome |
| spellingShingle | Jing Liu Tao Chen Chun-Lei Wang Xiao Liu Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency Genes pear rootstock boron deficiency aquaporin plant hormone transcriptome |
| title | Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency |
| title_full | Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency |
| title_fullStr | Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency |
| title_full_unstemmed | Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency |
| title_short | Transcriptome Analysis in <i>Pyrus betulaefolia</i> Roots in Response to Short-Term Boron Deficiency |
| title_sort | transcriptome analysis in i pyrus betulaefolia i roots in response to short term boron deficiency |
| topic | pear rootstock boron deficiency aquaporin plant hormone transcriptome |
| url | https://www.mdpi.com/2073-4425/14/4/817 |
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