Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility
Objective: Although the role of obesity and diabetes mellitus (DM) in male infertility is well established, little informationabout the underlying cellular mechanisms in infertility is available. In this sense, nuclear factor kappa-B (NF-kB) hasbeen recognized as an important regulator in obesity an...
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| Format: | Article |
| Language: | English |
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Royan Institute (ACECR), Tehran
2023-01-01
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| Series: | Cell Journal |
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| Online Access: | https://www.celljournal.org/article_696708_aa8e5ce59c9eb7df4a9c905b6de739d2.pdf |
| _version_ | 1797946309724864512 |
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| author | Shima Abbasihormozi Azam Kouhkan Abdolhossein Shaverdi Mohammad Ali Sadighi Gilani Vahab Babapour Amir Niasari Naslji Vahid Akbarinehad AliReza Alizadeh |
| author_facet | Shima Abbasihormozi Azam Kouhkan Abdolhossein Shaverdi Mohammad Ali Sadighi Gilani Vahab Babapour Amir Niasari Naslji Vahid Akbarinehad AliReza Alizadeh |
| author_sort | Shima Abbasihormozi |
| collection | DOAJ |
| description | Objective:
Although the role of obesity and diabetes mellitus (DM) in male infertility is well established, little informationabout the underlying cellular mechanisms in infertility is available. In this sense, nuclear factor kappa-B (NF-kB) hasbeen recognized as an important regulator in obesity and DM; However, its function in the pathogenesis of maleinfertility has never been studied in obese or men who suffer from diabetes. Therefore, the main goal of current researchis assessing NF-kB existence and activity in ejaculated human spermatozoa considering the obesity and diabeticscondition of males.
Materials and Methods:
In an experimental study, the ELISA technique was applied to analyze NF-kB levels in spermof four experimental groups: non-obese none-diabetic men (body mass index (BMI) <25 kg/m2; control group; n=30),obese non-diabetic men (BMI >30 kg/m2; OB group; n=30), non-obese diabetic men (BMI <25 kg/m2; DM group; n=30),and obese diabetic men (BMI >30 kg/m2; OB-DM group; n=30) who were presented to Royan Institute Infertility Center.In addition, protein localization was shown by Immunocytofluorescent assay. Sperm features were also evaluated usingCASA.
Results:
The diabetic men were older than non-diabetic men regardless of obesity status (P=0.0002). Sperm progressivemotility was affected by obesity (P=0.035) and type A sperm progressive motility was affected by DM (P=0.034). Theconcentration of sperm (P=0.013), motility (P=0.025) and morphology (P<0.0001) were altered by obesity × diabetesinteraction effects. The NF-kB activity was negatively influenced by the main impact of diabetics (P=0.019). Obesitydid not affect (P=0.248) NF-kB activity. Uniquely, NF-kB localized to the midpiece of sperm and post-acrosomal areas.
Conclusion:
The current study indicated a lower concentration of NF-kB in diabetic men, no effect of obesity on NF-kBwas observed yet. Additionally, we revealed the main obesity and diabetes effects, and their interaction effect adverselyinfluenced sperm characteristics. |
| first_indexed | 2024-04-10T21:09:01Z |
| format | Article |
| id | doaj.art-a18bff4ac1224a33bcf69c80e352cbde |
| institution | Directory Open Access Journal |
| issn | 2228-5806 2228-5814 |
| language | English |
| last_indexed | 2024-04-10T21:09:01Z |
| publishDate | 2023-01-01 |
| publisher | Royan Institute (ACECR), Tehran |
| record_format | Article |
| series | Cell Journal |
| spelling | doaj.art-a18bff4ac1224a33bcf69c80e352cbde2023-01-21T10:48:26ZengRoyan Institute (ACECR), TehranCell Journal2228-58062228-58142023-01-01251172410.22074/cellj.2022.557547.1065696708Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male InfertilityShima Abbasihormozi0Azam Kouhkan1Abdolhossein Shaverdi2Mohammad Ali Sadighi Gilani3Vahab Babapour4Amir Niasari Naslji5Vahid Akbarinehad6AliReza Alizadeh7Reproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranReproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranReproductive Epidemiology Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Andrology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranDepartment of Basic Science, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, IranDepartment of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, IranObjective: Although the role of obesity and diabetes mellitus (DM) in male infertility is well established, little informationabout the underlying cellular mechanisms in infertility is available. In this sense, nuclear factor kappa-B (NF-kB) hasbeen recognized as an important regulator in obesity and DM; However, its function in the pathogenesis of maleinfertility has never been studied in obese or men who suffer from diabetes. Therefore, the main goal of current researchis assessing NF-kB existence and activity in ejaculated human spermatozoa considering the obesity and diabeticscondition of males. Materials and Methods: In an experimental study, the ELISA technique was applied to analyze NF-kB levels in spermof four experimental groups: non-obese none-diabetic men (body mass index (BMI) <25 kg/m2; control group; n=30),obese non-diabetic men (BMI >30 kg/m2; OB group; n=30), non-obese diabetic men (BMI <25 kg/m2; DM group; n=30),and obese diabetic men (BMI >30 kg/m2; OB-DM group; n=30) who were presented to Royan Institute Infertility Center.In addition, protein localization was shown by Immunocytofluorescent assay. Sperm features were also evaluated usingCASA. Results: The diabetic men were older than non-diabetic men regardless of obesity status (P=0.0002). Sperm progressivemotility was affected by obesity (P=0.035) and type A sperm progressive motility was affected by DM (P=0.034). Theconcentration of sperm (P=0.013), motility (P=0.025) and morphology (P<0.0001) were altered by obesity × diabetesinteraction effects. The NF-kB activity was negatively influenced by the main impact of diabetics (P=0.019). Obesitydid not affect (P=0.248) NF-kB activity. Uniquely, NF-kB localized to the midpiece of sperm and post-acrosomal areas. Conclusion: The current study indicated a lower concentration of NF-kB in diabetic men, no effect of obesity on NF-kBwas observed yet. Additionally, we revealed the main obesity and diabetes effects, and their interaction effect adverselyinfluenced sperm characteristics.https://www.celljournal.org/article_696708_aa8e5ce59c9eb7df4a9c905b6de739d2.pdfdiabetes mellitusnuclear factor kappa-bobesity morbidspermatozoatype ii |
| spellingShingle | Shima Abbasihormozi Azam Kouhkan Abdolhossein Shaverdi Mohammad Ali Sadighi Gilani Vahab Babapour Amir Niasari Naslji Vahid Akbarinehad AliReza Alizadeh Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility Cell Journal diabetes mellitus nuclear factor kappa-b obesity morbid spermatozoa type ii |
| title | Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility |
| title_full | Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility |
| title_fullStr | Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility |
| title_full_unstemmed | Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility |
| title_short | Nuclear Factor Kappa-B Protein Levels in Sperm of Obese Men with and without Diabetes; Cellular Approach in Male Infertility |
| title_sort | nuclear factor kappa b protein levels in sperm of obese men with and without diabetes cellular approach in male infertility |
| topic | diabetes mellitus nuclear factor kappa-b obesity morbid spermatozoa type ii |
| url | https://www.celljournal.org/article_696708_aa8e5ce59c9eb7df4a9c905b6de739d2.pdf |
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