The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber
The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally-induced aestivation by a marine invertebrate more recently. In the present study, we hypothesized that miRNA-200-3p may contribute to establish rapid biological control to regulate fatty acid meta...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2018-10-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/5703.pdf |
| _version_ | 1797418268844097536 |
|---|---|
| author | Muyan Chen Shanshan Wang Xingke Li Kenneth B. Storey Xiumei Zhang |
| author_facet | Muyan Chen Shanshan Wang Xingke Li Kenneth B. Storey Xiumei Zhang |
| author_sort | Muyan Chen |
| collection | DOAJ |
| description | The sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally-induced aestivation by a marine invertebrate more recently. In the present study, we hypothesized that miRNA-200-3p may contribute to establish rapid biological control to regulate fatty acid metabolism during a estivation. The peroxisomal bi-functional enzyme (EHHADH) is a crucial participant of the classical peroxisomal fatty acid β-oxidation pathway, the relative mRNA transcripts and protein expressions of EHHADH were analyzed in intestine from sea cucumbers experienced long-term aestivation. Both mRNA transcripts and protein expressions of EHHADH in intestine decreased significantly during deep-aestivation as compared with non-aestivation controls. Analysis of the 3′ UTR of AjEHHADH showed the presence of a conserved binding site for miR-200-3p. Level of miR-200-3p showed an inverse correlation with EHHADH mRNA transcripts and protein levels in intestine, implicating miR-200-3p may directly targeted AjEHHADH by inducing the degradation of AjEHHADH mRNA in the aestivating sea cucumber, further dual-luciferase reporter assay validated the predicted role of miRNA-200-3p in regulating AjEHHADH. In order to further understand their regulatory mechanism, we conducted the functional experiment in vivo. The overexpression of miR-200-3p in sea cucumber significantly decreased mRNA and protein expression levels of AjEHHADH. Taken together, these findings suggested the potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber. |
| first_indexed | 2024-03-09T06:31:16Z |
| format | Article |
| id | doaj.art-a190de4074c94117a07516e3dfe219d0 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:16Z |
| publishDate | 2018-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-a190de4074c94117a07516e3dfe219d02023-12-03T11:05:38ZengPeerJ Inc.PeerJ2167-83592018-10-016e570310.7717/peerj.5703The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumberMuyan Chen0Shanshan Wang1Xingke Li2Kenneth B. Storey3Xiumei Zhang4Key Laboratory of Mariculture (Ocean University of China), Ministry of Education, Ocean University of China, Qingdao, ChinaKey Laboratory of Mariculture (Ocean University of China), Ministry of Education, Ocean University of China, Qingdao, ChinaKey Laboratory of Mariculture (Ocean University of China), Ministry of Education, Ocean University of China, Qingdao, ChinaInstitute of Biochemistry, Carleton University, Ottawa, CanadaKey Laboratory of Mariculture (Ocean University of China), Ministry of Education, Ocean University of China, Qingdao, ChinaThe sea cucumber (Apostichopus japonicus) has become a good model organism for studying environmentally-induced aestivation by a marine invertebrate more recently. In the present study, we hypothesized that miRNA-200-3p may contribute to establish rapid biological control to regulate fatty acid metabolism during a estivation. The peroxisomal bi-functional enzyme (EHHADH) is a crucial participant of the classical peroxisomal fatty acid β-oxidation pathway, the relative mRNA transcripts and protein expressions of EHHADH were analyzed in intestine from sea cucumbers experienced long-term aestivation. Both mRNA transcripts and protein expressions of EHHADH in intestine decreased significantly during deep-aestivation as compared with non-aestivation controls. Analysis of the 3′ UTR of AjEHHADH showed the presence of a conserved binding site for miR-200-3p. Level of miR-200-3p showed an inverse correlation with EHHADH mRNA transcripts and protein levels in intestine, implicating miR-200-3p may directly targeted AjEHHADH by inducing the degradation of AjEHHADH mRNA in the aestivating sea cucumber, further dual-luciferase reporter assay validated the predicted role of miRNA-200-3p in regulating AjEHHADH. In order to further understand their regulatory mechanism, we conducted the functional experiment in vivo. The overexpression of miR-200-3p in sea cucumber significantly decreased mRNA and protein expression levels of AjEHHADH. Taken together, these findings suggested the potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber.https://peerj.com/articles/5703.pdfSea cucumberFatty acid metabolismAjEHHADHAestivation |
| spellingShingle | Muyan Chen Shanshan Wang Xingke Li Kenneth B. Storey Xiumei Zhang The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber PeerJ Sea cucumber Fatty acid metabolism AjEHHADH Aestivation |
| title | The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber |
| title_full | The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber |
| title_fullStr | The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber |
| title_full_unstemmed | The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber |
| title_short | The potential contribution of miRNA-200-3p to the fatty acid metabolism by regulating AjEHHADH during aestivation in sea cucumber |
| title_sort | potential contribution of mirna 200 3p to the fatty acid metabolism by regulating ajehhadh during aestivation in sea cucumber |
| topic | Sea cucumber Fatty acid metabolism AjEHHADH Aestivation |
| url | https://peerj.com/articles/5703.pdf |
| work_keys_str_mv | AT muyanchen thepotentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT shanshanwang thepotentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT xingkeli thepotentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT kennethbstorey thepotentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT xiumeizhang thepotentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT muyanchen potentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT shanshanwang potentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT xingkeli potentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT kennethbstorey potentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber AT xiumeizhang potentialcontributionofmirna2003ptothefattyacidmetabolismbyregulatingajehhadhduringaestivationinseacucumber |