The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities
Honey exhibited potential antimicrobial activity against multidrug resistant (MDR) bacteria that continues to be a serious health problem. We reported the in-vitro activity of Saudi Sumra honey against clinical pathogenic bacteria and fungi, antibiofilm, anti-quorum-sensing (QS) and antioxidant acti...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/10/1212 |
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| author | Abdulrahman S. Bazaid Abdu Aldarhami Mitesh Patel Mohd Adnan Assia Hamdi Mejdi Snoussi Husam Qanash Mohammed Imam Mohammad Khalil Monjed Aiah Mustafa Khateb |
| author_facet | Abdulrahman S. Bazaid Abdu Aldarhami Mitesh Patel Mohd Adnan Assia Hamdi Mejdi Snoussi Husam Qanash Mohammed Imam Mohammad Khalil Monjed Aiah Mustafa Khateb |
| author_sort | Abdulrahman S. Bazaid |
| collection | DOAJ |
| description | Honey exhibited potential antimicrobial activity against multidrug resistant (MDR) bacteria that continues to be a serious health problem. We reported the in-vitro activity of Saudi Sumra honey against clinical pathogenic bacteria and fungi, antibiofilm, anti-quorum-sensing (QS) and antioxidant activities in relation to its phytochemical composition assessed by gas chromatography-mass spectrometry (GC-MS). Broth dilution method and scavenging activities against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and <i>β</i>-carotene bleaching assays were performed. The GC-MS analysis of Sumra honey showed that 2,4-dihydroxy-2,5-dimethyl-3(2H)-furan-3-one 1-methylcyclopropanemethanol were the major identified phytoconstituents. Sumra honey showed a minimum inhibitory concentration (MIC) to clinical isolates of <i>Staphylococcus aureus</i> including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) at 300 mg/mL, <i>Pseudomonas aeruginosa</i> (250 mg/mL), <i>Escherichia coli</i> (350 mg/mL) and <i>Acinetobacter baumannii</i> (250 mg/mL); clinical fungal isolates—<i>Candida auris</i> (600 mg/mL) and <i>Cryptococcus neoformans</i> (>1000 mg/mL); wild type fungal isolates—<i>Candida krusei</i> (>1000 mg/mL) and <i>Candida albicans</i> (700 mg/mL). In addition, Sumra honey demonstrated promising inhibition targeting biofilm formation by 59% for <i>Bacillus subtilis</i>, 48% for <i>S. aureus</i>, 38% for <i>E. coli</i>, and 33.63% for <i>P. aeruginosa</i>. The violacein production in <i>Chromobacterium violaceum</i> was reduced to 68%, whereas pyocyanin production in <i>P. aeruginosa</i> was reduced to 54.86% at ½ MIC. Furthermore, Sumra honey exhibited strong antioxidant activities (DPPH − IC<sub>50</sub> = 7.7 mg/mL; ABTS − IC<sub>50</sub> = 5.4 mg/mL; <i>β</i>-carotene − IC<sub>50</sub> = >20 mg/mL). Overall, obtained data highlighted the promising potential therapeutic use of Sumra honey treating infections caused by MDR bacteria and fungi. Moreover, Sumra honey can be a good candidate as an inhibitor agent for bacterial cellular communication in strains of <i>P. aeruginosa</i> and <i>C. violaceum</i>. |
| first_indexed | 2024-03-09T19:36:45Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-09T19:36:45Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
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| series | Pharmaceuticals |
| spelling | doaj.art-a1948522f5904e5b802658d38c533fd82023-11-24T01:51:09ZengMDPI AGPharmaceuticals1424-82472022-09-011510121210.3390/ph15101212The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant ActivitiesAbdulrahman S. Bazaid0Abdu Aldarhami1Mitesh Patel2Mohd Adnan3Assia Hamdi4Mejdi Snoussi5Husam Qanash6Mohammed Imam7Mohammad Khalil Monjed8Aiah Mustafa Khateb9Department of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Medical Microbiology, Qunfudah Faculty of Medicine, Umm Al-Qura University, Al-Qunfudah 21961, Saudi ArabiaDepartment of Biotechnology, Parul Institute of Applied Sciences and Centre of Research for Development, Parul University, Vadodara 391760, IndiaDepartment of Biology, College of Science, University of Ha’il, Hail 81451, Saudi ArabiaLaboratory of Chemical, Pharmaceutical and Pharmacological Development of Drugs, Faculty of Pharmacy, Monastir 5000, TunisiaDepartment of Biology, College of Science, University of Ha’il, Hail 81451, Saudi ArabiaDepartment of Medical Laboratory Science, College of Applied Medical Sciences, University of Ha’il, Hail 55476, Saudi ArabiaDepartment of Medical Microbiology, Qunfudah Faculty of Medicine, Umm Al-Qura University, Al-Qunfudah 21961, Saudi ArabiaDepartment of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah 21961, Saudi ArabiaMedical Laboratory Technology Department, College of Applied Medical Science, Taibah University, Madinah 42353, Saudi ArabiaHoney exhibited potential antimicrobial activity against multidrug resistant (MDR) bacteria that continues to be a serious health problem. We reported the in-vitro activity of Saudi Sumra honey against clinical pathogenic bacteria and fungi, antibiofilm, anti-quorum-sensing (QS) and antioxidant activities in relation to its phytochemical composition assessed by gas chromatography-mass spectrometry (GC-MS). Broth dilution method and scavenging activities against 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and <i>β</i>-carotene bleaching assays were performed. The GC-MS analysis of Sumra honey showed that 2,4-dihydroxy-2,5-dimethyl-3(2H)-furan-3-one 1-methylcyclopropanemethanol were the major identified phytoconstituents. Sumra honey showed a minimum inhibitory concentration (MIC) to clinical isolates of <i>Staphylococcus aureus</i> including methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) at 300 mg/mL, <i>Pseudomonas aeruginosa</i> (250 mg/mL), <i>Escherichia coli</i> (350 mg/mL) and <i>Acinetobacter baumannii</i> (250 mg/mL); clinical fungal isolates—<i>Candida auris</i> (600 mg/mL) and <i>Cryptococcus neoformans</i> (>1000 mg/mL); wild type fungal isolates—<i>Candida krusei</i> (>1000 mg/mL) and <i>Candida albicans</i> (700 mg/mL). In addition, Sumra honey demonstrated promising inhibition targeting biofilm formation by 59% for <i>Bacillus subtilis</i>, 48% for <i>S. aureus</i>, 38% for <i>E. coli</i>, and 33.63% for <i>P. aeruginosa</i>. The violacein production in <i>Chromobacterium violaceum</i> was reduced to 68%, whereas pyocyanin production in <i>P. aeruginosa</i> was reduced to 54.86% at ½ MIC. Furthermore, Sumra honey exhibited strong antioxidant activities (DPPH − IC<sub>50</sub> = 7.7 mg/mL; ABTS − IC<sub>50</sub> = 5.4 mg/mL; <i>β</i>-carotene − IC<sub>50</sub> = >20 mg/mL). Overall, obtained data highlighted the promising potential therapeutic use of Sumra honey treating infections caused by MDR bacteria and fungi. Moreover, Sumra honey can be a good candidate as an inhibitor agent for bacterial cellular communication in strains of <i>P. aeruginosa</i> and <i>C. violaceum</i>.https://www.mdpi.com/1424-8247/15/10/1212Sumra honeyantibacterialantifungalantioxidantsantibiofilmanti-quorum-sensing |
| spellingShingle | Abdulrahman S. Bazaid Abdu Aldarhami Mitesh Patel Mohd Adnan Assia Hamdi Mejdi Snoussi Husam Qanash Mohammed Imam Mohammad Khalil Monjed Aiah Mustafa Khateb The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities Pharmaceuticals Sumra honey antibacterial antifungal antioxidants antibiofilm anti-quorum-sensing |
| title | The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities |
| title_full | The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities |
| title_fullStr | The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities |
| title_full_unstemmed | The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities |
| title_short | The Antimicrobial Effects of Saudi Sumra Honey against Drug Resistant Pathogens: Phytochemical Analysis, Antibiofilm, Anti-Quorum Sensing, and Antioxidant Activities |
| title_sort | antimicrobial effects of saudi sumra honey against drug resistant pathogens phytochemical analysis antibiofilm anti quorum sensing and antioxidant activities |
| topic | Sumra honey antibacterial antifungal antioxidants antibiofilm anti-quorum-sensing |
| url | https://www.mdpi.com/1424-8247/15/10/1212 |
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