(18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers.
AIM: To study whether (18)F-FDG can be used for in vivo imaging of atherogenesis by examining the correlation between (18)F-FDG uptake and gene expression of key molecular markers of atherosclerosis in apoE(-/-) mice. METHODS: Nine groups of apoE(-/-) mice were given normal chow or high-fat diet. At...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2012-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3511408?pdf=render |
| _version_ | 1818855807682347008 |
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| author | Anne Mette Fisker Hag Sune Folke Pedersen Christina Christoffersen Tina Binderup Mette Munk Jensen Jesper Tranekjær Jørgensen Dorthe Skovgaard Rasmus Sejersten Ripa Andreas Kjaer |
| author_facet | Anne Mette Fisker Hag Sune Folke Pedersen Christina Christoffersen Tina Binderup Mette Munk Jensen Jesper Tranekjær Jørgensen Dorthe Skovgaard Rasmus Sejersten Ripa Andreas Kjaer |
| author_sort | Anne Mette Fisker Hag |
| collection | DOAJ |
| description | AIM: To study whether (18)F-FDG can be used for in vivo imaging of atherogenesis by examining the correlation between (18)F-FDG uptake and gene expression of key molecular markers of atherosclerosis in apoE(-/-) mice. METHODS: Nine groups of apoE(-/-) mice were given normal chow or high-fat diet. At different time-points, (18)F-FDG PET/contrast-enhanced CT scans were performed on dedicated animal scanners. After scans, animals were euthanized, aortas removed, gamma counted, RNA extracted from the tissue, and gene expression of chemo (C-X-C motif) ligand 1 (CXCL-1), monocyte chemoattractant protein (MCP)-1, vascular cell adhesion molecule (VCAM)-1, cluster of differentiation molecule (CD)-68, osteopontin (OPN), lectin-like oxidized LDL-receptor (LOX)-1, hypoxia-inducible factor (HIF)-1α, HIF-2α, vascular endothelial growth factor A (VEGF), and tissue factor (TF) was measured by means of qPCR. RESULTS: The uptake of (18)F-FDG increased over time in the groups of mice receiving high-fat diet measured by PET and ex vivo gamma counting. The gene expression of all examined markers of atherosclerosis correlated significantly with (18)F-FDG uptake. The strongest correlation was seen with TF and CD68 (p<0.001). A multivariate analysis showed CD68, OPN, TF, and VCAM-1 to be the most important contributors to the uptake of (18)F-FDG. Together they could explain 60% of the (18)F-FDG uptake. CONCLUSION: We have demonstrated that (18)F-FDG can be used to follow the progression of atherosclerosis in apoE(-/-) mice. The gene expression of ten molecular markers representing different molecular processes important for atherosclerosis was shown to correlate with the uptake of (18)F-FDG. Especially, the gene expressions of CD68, OPN, TF, and VCAM-1 were strong predictors for the uptake. |
| first_indexed | 2024-12-19T08:14:29Z |
| format | Article |
| id | doaj.art-a194bfd869b541d2b213568a03336722 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T08:14:29Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a194bfd869b541d2b213568a033367222022-12-21T20:29:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e5090810.1371/journal.pone.0050908(18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers.Anne Mette Fisker HagSune Folke PedersenChristina ChristoffersenTina BinderupMette Munk JensenJesper Tranekjær JørgensenDorthe SkovgaardRasmus Sejersten RipaAndreas KjaerAIM: To study whether (18)F-FDG can be used for in vivo imaging of atherogenesis by examining the correlation between (18)F-FDG uptake and gene expression of key molecular markers of atherosclerosis in apoE(-/-) mice. METHODS: Nine groups of apoE(-/-) mice were given normal chow or high-fat diet. At different time-points, (18)F-FDG PET/contrast-enhanced CT scans were performed on dedicated animal scanners. After scans, animals were euthanized, aortas removed, gamma counted, RNA extracted from the tissue, and gene expression of chemo (C-X-C motif) ligand 1 (CXCL-1), monocyte chemoattractant protein (MCP)-1, vascular cell adhesion molecule (VCAM)-1, cluster of differentiation molecule (CD)-68, osteopontin (OPN), lectin-like oxidized LDL-receptor (LOX)-1, hypoxia-inducible factor (HIF)-1α, HIF-2α, vascular endothelial growth factor A (VEGF), and tissue factor (TF) was measured by means of qPCR. RESULTS: The uptake of (18)F-FDG increased over time in the groups of mice receiving high-fat diet measured by PET and ex vivo gamma counting. The gene expression of all examined markers of atherosclerosis correlated significantly with (18)F-FDG uptake. The strongest correlation was seen with TF and CD68 (p<0.001). A multivariate analysis showed CD68, OPN, TF, and VCAM-1 to be the most important contributors to the uptake of (18)F-FDG. Together they could explain 60% of the (18)F-FDG uptake. CONCLUSION: We have demonstrated that (18)F-FDG can be used to follow the progression of atherosclerosis in apoE(-/-) mice. The gene expression of ten molecular markers representing different molecular processes important for atherosclerosis was shown to correlate with the uptake of (18)F-FDG. Especially, the gene expressions of CD68, OPN, TF, and VCAM-1 were strong predictors for the uptake.http://europepmc.org/articles/PMC3511408?pdf=render |
| spellingShingle | Anne Mette Fisker Hag Sune Folke Pedersen Christina Christoffersen Tina Binderup Mette Munk Jensen Jesper Tranekjær Jørgensen Dorthe Skovgaard Rasmus Sejersten Ripa Andreas Kjaer (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. PLoS ONE |
| title | (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. |
| title_full | (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. |
| title_fullStr | (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. |
| title_full_unstemmed | (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. |
| title_short | (18)F-FDG PET imaging of murine atherosclerosis: association with gene expression of key molecular markers. |
| title_sort | 18 f fdg pet imaging of murine atherosclerosis association with gene expression of key molecular markers |
| url | http://europepmc.org/articles/PMC3511408?pdf=render |
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