Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells
Fish side streams are an environmental and economic problem. In this work, pulsed electric fields (PEF) extraction was optimized and used as a new method for their valorization. Sea bass head, skin, viscera, and backbone were used. PEF technology (123–300 kJ/kg, 1–3 kV/cm) improved the extraction of...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/12/14/2717 |
| _version_ | 1797589317965578240 |
|---|---|
| author | Francisco J. Martí-Quijal Juan Manuel Castagnini María-José Ruiz Francisco J. Barba |
| author_facet | Francisco J. Martí-Quijal Juan Manuel Castagnini María-José Ruiz Francisco J. Barba |
| author_sort | Francisco J. Martí-Quijal |
| collection | DOAJ |
| description | Fish side streams are an environmental and economic problem. In this work, pulsed electric fields (PEF) extraction was optimized and used as a new method for their valorization. Sea bass head, skin, viscera, and backbone were used. PEF technology (123–300 kJ/kg, 1–3 kV/cm) improved the extraction of proteins and antioxidant compounds from head and skin, but was not successful for viscera. SDS-PAGE showed that the protein molecular weight distribution was affected by the extraction process, revealing differences between the control and PEF extraction conditions. In addition, the extraction of macro-minerals and micro-minerals were also evaluated. The effect of PEF differed according to the matrix and the mineral studied. Heavy metals were also taken into account, studying the presence of As, Cd, Hg, and Pb in the extracts. PEF pre-treatment reduced the presence of As in skin, viscera, and backbone, ranging from 18.25 to 28.48% according to the matrix evaluated. The analysis of potential antioxidant bioactive peptides showed that the treatment of the sample directly influenced their variety. Additionally, the extracts obtained from the head were found to increase cell viability when tested on SH-SY5Y cells. In conclusion, PEF extraction can be a useful tool for the valorization of fish side streams. |
| first_indexed | 2024-03-11T01:04:48Z |
| format | Article |
| id | doaj.art-a196aba8a5d3485984f2eae43061b70c |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T01:04:48Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-a196aba8a5d3485984f2eae43061b70c2023-11-18T19:20:21ZengMDPI AGFoods2304-81582023-07-011214271710.3390/foods12142717Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y CellsFrancisco J. Martí-Quijal0Juan Manuel Castagnini1María-José Ruiz2Francisco J. Barba3Research Group in Innovative Technologies for Sustainable Food (ALISOST), Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, SpainResearch Group in Innovative Technologies for Sustainable Food (ALISOST), Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, SpainResearch Group in Alternative Methods for Determining TOXICS Effects and Risk Assessment of Contaminants and Mixtures (RiskTox), Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, SpainResearch Group in Innovative Technologies for Sustainable Food (ALISOST), Preventive Medicine and Public Health, Food Science, Toxicology and Forensic Medicine Department, Faculty of Pharmacy, Universitat de València, Avda. Vicent Andrés Estellés, s/n, 46100 Burjassot, SpainFish side streams are an environmental and economic problem. In this work, pulsed electric fields (PEF) extraction was optimized and used as a new method for their valorization. Sea bass head, skin, viscera, and backbone were used. PEF technology (123–300 kJ/kg, 1–3 kV/cm) improved the extraction of proteins and antioxidant compounds from head and skin, but was not successful for viscera. SDS-PAGE showed that the protein molecular weight distribution was affected by the extraction process, revealing differences between the control and PEF extraction conditions. In addition, the extraction of macro-minerals and micro-minerals were also evaluated. The effect of PEF differed according to the matrix and the mineral studied. Heavy metals were also taken into account, studying the presence of As, Cd, Hg, and Pb in the extracts. PEF pre-treatment reduced the presence of As in skin, viscera, and backbone, ranging from 18.25 to 28.48% according to the matrix evaluated. The analysis of potential antioxidant bioactive peptides showed that the treatment of the sample directly influenced their variety. Additionally, the extracts obtained from the head were found to increase cell viability when tested on SH-SY5Y cells. In conclusion, PEF extraction can be a useful tool for the valorization of fish side streams.https://www.mdpi.com/2304-8158/12/14/2717pulsed electric fieldsfish side streamsantioxidantsmineralsheavy metalsbioactive peptides |
| spellingShingle | Francisco J. Martí-Quijal Juan Manuel Castagnini María-José Ruiz Francisco J. Barba Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells Foods pulsed electric fields fish side streams antioxidants minerals heavy metals bioactive peptides |
| title | Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells |
| title_full | Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells |
| title_fullStr | Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells |
| title_full_unstemmed | Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells |
| title_short | Sea Bass Side Streams Extracts Obtained by Pulsed Electric Fields: Nutritional Characterization and Effect on SH-SY5Y Cells |
| title_sort | sea bass side streams extracts obtained by pulsed electric fields nutritional characterization and effect on sh sy5y cells |
| topic | pulsed electric fields fish side streams antioxidants minerals heavy metals bioactive peptides |
| url | https://www.mdpi.com/2304-8158/12/14/2717 |
| work_keys_str_mv | AT franciscojmartiquijal seabasssidestreamsextractsobtainedbypulsedelectricfieldsnutritionalcharacterizationandeffectonshsy5ycells AT juanmanuelcastagnini seabasssidestreamsextractsobtainedbypulsedelectricfieldsnutritionalcharacterizationandeffectonshsy5ycells AT mariajoseruiz seabasssidestreamsextractsobtainedbypulsedelectricfieldsnutritionalcharacterizationandeffectonshsy5ycells AT franciscojbarba seabasssidestreamsextractsobtainedbypulsedelectricfieldsnutritionalcharacterizationandeffectonshsy5ycells |