MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL
SUMMARY In this study, Leishmaniaspecies were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universidade de São Paulo (USP)
2015-08-01
|
| Series: | Revista do Instituto de Medicina Tropical de São Paulo |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000400343&lng=en&tlng=en |
| _version_ | 1827269407819169792 |
|---|---|
| author | Carolina Bioni Garcia TELES Jansen Fernandes MEDEIROS Ana Paula de Azevedo dos SANTOS Luís Antônio Rodrigues de FREITAS Tony Hiroshi KATSURAGAWA Lilian Motta CANTANHÊDE Ricardo de Godoi Mattos FERREIRA Luís Marcelo Aranha CAMARG |
| author_facet | Carolina Bioni Garcia TELES Jansen Fernandes MEDEIROS Ana Paula de Azevedo dos SANTOS Luís Antônio Rodrigues de FREITAS Tony Hiroshi KATSURAGAWA Lilian Motta CANTANHÊDE Ricardo de Godoi Mattos FERREIRA Luís Marcelo Aranha CAMARG |
| author_sort | Carolina Bioni Garcia TELES |
| collection | DOAJ |
| description | SUMMARY In this study, Leishmaniaspecies were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR, the DNA of Leishmaniawas detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis (3.1%)This is the first report of L. (V.) shawiand L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting. |
| first_indexed | 2025-03-22T05:05:03Z |
| format | Article |
| id | doaj.art-a19a442f4df84d31b51d4d12e868fee5 |
| institution | Directory Open Access Journal |
| issn | 1678-9946 |
| language | English |
| last_indexed | 2025-03-22T05:05:03Z |
| publishDate | 2015-08-01 |
| publisher | Universidade de São Paulo (USP) |
| record_format | Article |
| series | Revista do Instituto de Medicina Tropical de São Paulo |
| spelling | doaj.art-a19a442f4df84d31b51d4d12e868fee52024-04-27T12:20:46ZengUniversidade de São Paulo (USP)Revista do Instituto de Medicina Tropical de São Paulo1678-99462015-08-0157434334710.1590/S0036-46652015000400012S0036-46652015000400343MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZILCarolina Bioni Garcia TELESJansen Fernandes MEDEIROSAna Paula de Azevedo dos SANTOSLuís Antônio Rodrigues de FREITASTony Hiroshi KATSURAGAWALilian Motta CANTANHÊDERicardo de Godoi Mattos FERREIRALuís Marcelo Aranha CAMARGSUMMARY In this study, Leishmaniaspecies were identified by Polymerase Chain Reaction (PCR). The epidemiology of patients suspected of having American Cutaneous Leishmaniasis in the municipality of Assis Brasil, Acre State, located in the Brazil/Peru/Bolivia triborder was also investigated. By PCR, the DNA of Leishmaniawas detected in 100% of the cases (37 samples) and a PCR-Restriction Fragment Length Polymorphism (RFLP) of the hsp 70gene identified the species in 32 samples: Leishmania (Viannia) braziliensis (65.6%) , L. (V.) shawi (28.1%) , L. (V.) guyanensis (3.1%) and mixed infection L. (V.) guyanensis and L. (Leishmania) amazonensis (3.1%)This is the first report of L. (V.) shawiand L. (L.) amazonensis in Acre. The two predominant species were found in patients living in urban and rural areas. Most cases were found in males living in rural areas for at least three years and involved in rural work. This suggests, in most cases, a possible transmission of the disease from a rural/forest source, although some patients had not engaged in activities associated with permanence in forestall areas, which indicate a possible sandflies adaptation to the periurban setting.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000400343&lng=en&tlng=enLeishmaniaAcre StatePCR |
| spellingShingle | Carolina Bioni Garcia TELES Jansen Fernandes MEDEIROS Ana Paula de Azevedo dos SANTOS Luís Antônio Rodrigues de FREITAS Tony Hiroshi KATSURAGAWA Lilian Motta CANTANHÊDE Ricardo de Godoi Mattos FERREIRA Luís Marcelo Aranha CAMARG MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL Revista do Instituto de Medicina Tropical de São Paulo Leishmania Acre State PCR |
| title | MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL |
| title_full | MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL |
| title_fullStr | MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL |
| title_full_unstemmed | MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL |
| title_short | MOLECULAR CHARACTERIZATION OF AMERICAN CUTANEOUS LEISHMANIASIS IN THE TRI‑BORDER AREA OF ASSIS BRASIL, ACRE STATE, BRAZIL |
| title_sort | molecular characterization of american cutaneous leishmaniasis in the tri border area of assis brasil acre state brazil |
| topic | Leishmania Acre State PCR |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652015000400343&lng=en&tlng=en |
| work_keys_str_mv | AT carolinabionigarciateles molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT jansenfernandesmedeiros molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT anapauladeazevedodossantos molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT luisantoniorodriguesdefreitas molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT tonyhiroshikatsuragawa molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT lilianmottacantanhede molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT ricardodegodoimattosferreira molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil AT luismarceloaranhacamarg molecularcharacterizationofamericancutaneousleishmaniasisinthetriborderareaofassisbrasilacrestatebrazil |