Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice
Gastrointestinal ischemia/reperfusion (I/R) injury occurs in multiple clinical situations. We examined effects of zinc carnosine (ZnC), a health food supplement claimed to possess gastrointestinal supportive activity, on I/R injury. AGS, RIE and Caco-2 cells exposed to hypoxia/normoxia (H/N) reduced...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464621000104 |
| _version_ | 1818598304472104960 |
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| author | Raymond J. Playford Tania Marchbank |
| author_facet | Raymond J. Playford Tania Marchbank |
| author_sort | Raymond J. Playford |
| collection | DOAJ |
| description | Gastrointestinal ischemia/reperfusion (I/R) injury occurs in multiple clinical situations. We examined effects of zinc carnosine (ZnC), a health food supplement claimed to possess gastrointestinal supportive activity, on I/R injury. AGS, RIE and Caco-2 cells exposed to hypoxia/normoxia (H/N) reduced cell viability by 50%. ZnC given prior- or during-hypoxia improved survival by 50–70% (p < 0.01) and truncated increase in transepithelial permeability (HRP passage) by 50% whereas carnosine or ZnSO4 were ineffective. Induction of mesenteric I/R injury in mice caused severe jejunal mucosal and lung injury, increased lipid peroxidation (MDA), inflammatory infiltration (MPO) and circulating LPS and cytokines. ZnC (58 mg/kg/day, oral 7 days) reduced injury by 50%, reduced pro-apoptotic Caspase 3, 9 and Baxα, increased Bcl2 and Hsp70, and maintained tight junction ZO1 and Claudin1 levels (all p < 0.01 vs I/R alone). ZnC shows benefit over using zinc salt or carnosine. Oral ZnC may be a useful clinical approach for I/R injury. |
| first_indexed | 2024-12-16T12:01:34Z |
| format | Article |
| id | doaj.art-a1a163fd751f48babc03631f5ce09c2b |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-16T12:01:34Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-a1a163fd751f48babc03631f5ce09c2b2022-12-21T22:32:26ZengElsevierJournal of Functional Foods1756-46462021-03-0178104361Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in miceRaymond J. Playford0Tania Marchbank1Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine, Queen Mary, University of London, UKCorresponding author at: Centre for Immunobiology, The Blizard Institute Queen Mary University of London, Barts & The London School of Medicine and Dentistry, 4 Newark Street, London E1 2AT, UK.; Centre for Immunobiology, Blizard Institute, Barts and The London School of Medicine, Queen Mary, University of London, UKGastrointestinal ischemia/reperfusion (I/R) injury occurs in multiple clinical situations. We examined effects of zinc carnosine (ZnC), a health food supplement claimed to possess gastrointestinal supportive activity, on I/R injury. AGS, RIE and Caco-2 cells exposed to hypoxia/normoxia (H/N) reduced cell viability by 50%. ZnC given prior- or during-hypoxia improved survival by 50–70% (p < 0.01) and truncated increase in transepithelial permeability (HRP passage) by 50% whereas carnosine or ZnSO4 were ineffective. Induction of mesenteric I/R injury in mice caused severe jejunal mucosal and lung injury, increased lipid peroxidation (MDA), inflammatory infiltration (MPO) and circulating LPS and cytokines. ZnC (58 mg/kg/day, oral 7 days) reduced injury by 50%, reduced pro-apoptotic Caspase 3, 9 and Baxα, increased Bcl2 and Hsp70, and maintained tight junction ZO1 and Claudin1 levels (all p < 0.01 vs I/R alone). ZnC shows benefit over using zinc salt or carnosine. Oral ZnC may be a useful clinical approach for I/R injury.http://www.sciencedirect.com/science/article/pii/S1756464621000104RepairGut damageApoptosisHealth food supplementsBioactive peptide |
| spellingShingle | Raymond J. Playford Tania Marchbank Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice Journal of Functional Foods Repair Gut damage Apoptosis Health food supplements Bioactive peptide |
| title | Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice |
| title_full | Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice |
| title_fullStr | Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice |
| title_full_unstemmed | Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice |
| title_short | Oral zinc carnosine reduces multi-organ damage caused by gut ischemia/reperfusion in mice |
| title_sort | oral zinc carnosine reduces multi organ damage caused by gut ischemia reperfusion in mice |
| topic | Repair Gut damage Apoptosis Health food supplements Bioactive peptide |
| url | http://www.sciencedirect.com/science/article/pii/S1756464621000104 |
| work_keys_str_mv | AT raymondjplayford oralzinccarnosinereducesmultiorgandamagecausedbygutischemiareperfusioninmice AT taniamarchbank oralzinccarnosinereducesmultiorgandamagecausedbygutischemiareperfusioninmice |