Production of Large-Ring Cyclodextrins by Amylomaltases
Amylomaltase is a well-known glucan transferase that can produce large ring cyclodextrins (LR-CDs) or so-called cycloamyloses via cyclization reaction. Amylomaltases have been found in several microorganisms and their optimum temperatures are generally around 60–70 °C for thermostable amylomaltases...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/4/1446 |
| _version_ | 1827653524810366976 |
|---|---|
| author | Kuakarun Krusong Abbas Ismail Karan Wangpaiboon Piamsook Pongsawasdi |
| author_facet | Kuakarun Krusong Abbas Ismail Karan Wangpaiboon Piamsook Pongsawasdi |
| author_sort | Kuakarun Krusong |
| collection | DOAJ |
| description | Amylomaltase is a well-known glucan transferase that can produce large ring cyclodextrins (LR-CDs) or so-called cycloamyloses via cyclization reaction. Amylomaltases have been found in several microorganisms and their optimum temperatures are generally around 60–70 °C for thermostable amylomaltases and 30–45 °C for the enzymes from mesophilic bacteria and plants. The optimum pHs for mesophilic amylomaltases are around pH 6.0–7.0, while the thermostable amylomaltases are generally active at more acidic conditions. Size of LR-CDs depends on the source of amylomaltases and the reaction conditions including pH, temperature, incubation time, and substrate. For example, in the case of amylomaltase from <i>Corynebacterium glutamicum</i>, LR-CD productions at alkaline pH or at a long incubation time favored products with a low degree of polymerization. In this review, we explore the synthesis of LR-CDs by amylomaltases, structural information of amylomaltases, as well as current applications of LR-CDs and amylomaltases. |
| first_indexed | 2024-03-09T21:20:02Z |
| format | Article |
| id | doaj.art-a1a3e480707944bda79d4d10368f204b |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T21:20:02Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a1a3e480707944bda79d4d10368f204b2023-11-23T21:24:05ZengMDPI AGMolecules1420-30492022-02-01274144610.3390/molecules27041446Production of Large-Ring Cyclodextrins by AmylomaltasesKuakarun Krusong0Abbas Ismail1Karan Wangpaiboon2Piamsook Pongsawasdi3Structural and Computational Biology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330, ThailandStructural and Computational Biology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330, ThailandStructural and Computational Biology Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330, ThailandStarch and Cyclodextrin Research Unit, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phyathai Rd., Patumwan, Bangkok 10330, ThailandAmylomaltase is a well-known glucan transferase that can produce large ring cyclodextrins (LR-CDs) or so-called cycloamyloses via cyclization reaction. Amylomaltases have been found in several microorganisms and their optimum temperatures are generally around 60–70 °C for thermostable amylomaltases and 30–45 °C for the enzymes from mesophilic bacteria and plants. The optimum pHs for mesophilic amylomaltases are around pH 6.0–7.0, while the thermostable amylomaltases are generally active at more acidic conditions. Size of LR-CDs depends on the source of amylomaltases and the reaction conditions including pH, temperature, incubation time, and substrate. For example, in the case of amylomaltase from <i>Corynebacterium glutamicum</i>, LR-CD productions at alkaline pH or at a long incubation time favored products with a low degree of polymerization. In this review, we explore the synthesis of LR-CDs by amylomaltases, structural information of amylomaltases, as well as current applications of LR-CDs and amylomaltases.https://www.mdpi.com/1420-3049/27/4/1446amylomaltasecyclization4-α-glucanotransferaseslarge-ring cyclodextrinstarch |
| spellingShingle | Kuakarun Krusong Abbas Ismail Karan Wangpaiboon Piamsook Pongsawasdi Production of Large-Ring Cyclodextrins by Amylomaltases Molecules amylomaltase cyclization 4-α-glucanotransferases large-ring cyclodextrin starch |
| title | Production of Large-Ring Cyclodextrins by Amylomaltases |
| title_full | Production of Large-Ring Cyclodextrins by Amylomaltases |
| title_fullStr | Production of Large-Ring Cyclodextrins by Amylomaltases |
| title_full_unstemmed | Production of Large-Ring Cyclodextrins by Amylomaltases |
| title_short | Production of Large-Ring Cyclodextrins by Amylomaltases |
| title_sort | production of large ring cyclodextrins by amylomaltases |
| topic | amylomaltase cyclization 4-α-glucanotransferases large-ring cyclodextrin starch |
| url | https://www.mdpi.com/1420-3049/27/4/1446 |
| work_keys_str_mv | AT kuakarunkrusong productionoflargeringcyclodextrinsbyamylomaltases AT abbasismail productionoflargeringcyclodextrinsbyamylomaltases AT karanwangpaiboon productionoflargeringcyclodextrinsbyamylomaltases AT piamsookpongsawasdi productionoflargeringcyclodextrinsbyamylomaltases |