Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters
Nanotechnology holds significant ameliorative potential against neurodegenerative diseases, as it can protect the therapeutic substance and allow for its sustained release. In this study, the reducing and capping agents of <i>Urtica dioica</i> (UD), <i>Matricaria chamomilla</i&g...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/9/1679 |
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| author | Shabnam Shabir Amit Sehgal Joydeep Dutta Inderpal Devgon Sandeep K. Singh Walaa F. Alsanie Abdulhakeem S. Alamri Majid Alhomrani Abdulaziz Alsharif Mohammed Abubaker Mohammed Basalamah Hani Faidah Farkad Bantun Abdullah Ali Saati Emanuel Vamanu Mahendra P. Singh |
| author_facet | Shabnam Shabir Amit Sehgal Joydeep Dutta Inderpal Devgon Sandeep K. Singh Walaa F. Alsanie Abdulhakeem S. Alamri Majid Alhomrani Abdulaziz Alsharif Mohammed Abubaker Mohammed Basalamah Hani Faidah Farkad Bantun Abdullah Ali Saati Emanuel Vamanu Mahendra P. Singh |
| author_sort | Shabnam Shabir |
| collection | DOAJ |
| description | Nanotechnology holds significant ameliorative potential against neurodegenerative diseases, as it can protect the therapeutic substance and allow for its sustained release. In this study, the reducing and capping agents of <i>Urtica dioica</i> (UD), <i>Matricaria chamomilla</i> (MC), and <i>Murraya koenigii</i> (MK) extracts were used to synthesize bio-mediated zinc oxide nanoparticles (ZnO-NPs) against bacteria (<i>Staphylococcus aureus</i> and <i>Escherichia coli</i>) and against rotenone-induced toxicities in <i>D. melanogaster</i> for the first time. Their optical and structural properties were analyzed via FT-IR, DLS, XRD, EDS, SEM, UV–Vis, and zeta potential. The antioxidant and antimicrobial properties of the fabricated ZnO-NPs were evaluated employing cell-free models (DPPH and ABTS) and the well diffusion method, respectively. Rotenone (500 µM) was administered to <i>Drosophila</i> third instar larvae and freshly emerged flies for 24–120 h, either alone or in combination with plant extracts (UD, MC, an MK) and their biogenic ZnO-NPs. A comparative study on the protective effects of synthesized NPs was undertaken against rotenone-induced neurotoxic, cytotoxic, and behavioral alterations using an acetylcholinesterase inhibition assay, dye exclusion test, and locomotor parameters. The findings revealed that among the plant-derived ZnO-NPs, MK-ZnO NPs exhibit strong antimicrobial and antioxidant activities, followed by UD-ZnO NPs and MC-ZnO NPs. In this regard, ethno-nano medicinal therapeutic uses mimic similar effects in <i>D. melanogaster</i> by suppressing oxidative stress by restoring biochemical parameters (AchE and proteotoxicity activity) and lower cellular toxicity. These findings suggest that green-engineered ZnO-NPs have the potential to significantly enhance outcomes, with the promise of effective therapies for neurodegeneration, and could be used as a great alternative for clinical development. |
| first_indexed | 2024-03-10T23:06:42Z |
| format | Article |
| id | doaj.art-a1a67bb2bff1408eb7b30b7616300421 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T23:06:42Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a1a67bb2bff1408eb7b30b76163004212023-11-19T09:18:53ZengMDPI AGAntioxidants2076-39212023-08-01129167910.3390/antiox12091679Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral ParametersShabnam Shabir0Amit Sehgal1Joydeep Dutta2Inderpal Devgon3Sandeep K. Singh4Walaa F. Alsanie5Abdulhakeem S. Alamri6Majid Alhomrani7Abdulaziz Alsharif8Mohammed Abubaker Mohammed Basalamah9Hani Faidah10Farkad Bantun11Abdullah Ali Saati12Emanuel Vamanu13Mahendra P. Singh14School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, IndiaSchool of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, IndiaSchool of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, IndiaSchool of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, IndiaIndian Scientific Education and Technology Foundation, Lucknow 226002, Uttar Pradesh, IndiaDepartment of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Clinical Laboratory Sciences, The Faculty of Applied Medical Sciences, Taif University, Taif 21944, Saudi ArabiaDepartment of Pathology, Faculty of Medicine, Umm Al-Qura University, Makkah 24382, Saudi ArabiaDepartment of Microbiology, Faculty of Medicine, Umm Al-Qura University, Makkah 24382, Saudi ArabiaDepartment of Microbiology, Faculty of Medicine, Umm Al-Qura University, Makkah 24382, Saudi ArabiaDepartment of Community Medicine & Pilgrims Healthcare, Faculty of Medicine, Umm Al-Qura University, Makkah 24382, Saudi ArabiaFaculty of Biotechnology, University of Agricultural Sciences and Veterinary Medicine, 011464 Bucharest, RomaniaDepartment of Zoology and Centre of Genomics and Bioinformatics, DDU Gorakhpur University, Gorakhpur 273009, Uttar Pradesh, IndiaNanotechnology holds significant ameliorative potential against neurodegenerative diseases, as it can protect the therapeutic substance and allow for its sustained release. In this study, the reducing and capping agents of <i>Urtica dioica</i> (UD), <i>Matricaria chamomilla</i> (MC), and <i>Murraya koenigii</i> (MK) extracts were used to synthesize bio-mediated zinc oxide nanoparticles (ZnO-NPs) against bacteria (<i>Staphylococcus aureus</i> and <i>Escherichia coli</i>) and against rotenone-induced toxicities in <i>D. melanogaster</i> for the first time. Their optical and structural properties were analyzed via FT-IR, DLS, XRD, EDS, SEM, UV–Vis, and zeta potential. The antioxidant and antimicrobial properties of the fabricated ZnO-NPs were evaluated employing cell-free models (DPPH and ABTS) and the well diffusion method, respectively. Rotenone (500 µM) was administered to <i>Drosophila</i> third instar larvae and freshly emerged flies for 24–120 h, either alone or in combination with plant extracts (UD, MC, an MK) and their biogenic ZnO-NPs. A comparative study on the protective effects of synthesized NPs was undertaken against rotenone-induced neurotoxic, cytotoxic, and behavioral alterations using an acetylcholinesterase inhibition assay, dye exclusion test, and locomotor parameters. The findings revealed that among the plant-derived ZnO-NPs, MK-ZnO NPs exhibit strong antimicrobial and antioxidant activities, followed by UD-ZnO NPs and MC-ZnO NPs. In this regard, ethno-nano medicinal therapeutic uses mimic similar effects in <i>D. melanogaster</i> by suppressing oxidative stress by restoring biochemical parameters (AchE and proteotoxicity activity) and lower cellular toxicity. These findings suggest that green-engineered ZnO-NPs have the potential to significantly enhance outcomes, with the promise of effective therapies for neurodegeneration, and could be used as a great alternative for clinical development.https://www.mdpi.com/2076-3921/12/9/1679zinc oxide nanoparticlesantioxidantsantibacterial agentsmedicinal plantsgreen synthesisoxidative stress |
| spellingShingle | Shabnam Shabir Amit Sehgal Joydeep Dutta Inderpal Devgon Sandeep K. Singh Walaa F. Alsanie Abdulhakeem S. Alamri Majid Alhomrani Abdulaziz Alsharif Mohammed Abubaker Mohammed Basalamah Hani Faidah Farkad Bantun Abdullah Ali Saati Emanuel Vamanu Mahendra P. Singh Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters Antioxidants zinc oxide nanoparticles antioxidants antibacterial agents medicinal plants green synthesis oxidative stress |
| title | Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters |
| title_full | Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters |
| title_fullStr | Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters |
| title_full_unstemmed | Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters |
| title_short | Therapeutic Potential of Green-Engineered ZnO Nanoparticles on Rotenone-Exposed <i>D. melanogaster</i> (Oregon R<sup>+</sup>): Unveiling Ameliorated Biochemical, Cellular, and Behavioral Parameters |
| title_sort | therapeutic potential of green engineered zno nanoparticles on rotenone exposed i d melanogaster i oregon r sup sup unveiling ameliorated biochemical cellular and behavioral parameters |
| topic | zinc oxide nanoparticles antioxidants antibacterial agents medicinal plants green synthesis oxidative stress |
| url | https://www.mdpi.com/2076-3921/12/9/1679 |
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