The identification of archaeological eggshell using peptide markers
Avian eggshell survives well in alkaline and neutral soils, but its potential as an archaeological resource remains largely unexplored, mainly due to difficulties in its identification. Here we exploit the release of novel bird genomes and, for the first time on eggshell, use MALDI-ToF (matrix-assis...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2017-01-01
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| Series: | Science and Technology of Archaeological Research |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/20548923.2018.1424300 |
| _version_ | 1831761407474925568 |
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| author | Samantha Presslee Julie Wilson Jos Woolley Julia Best Douglas Russell Anita Radini Roman Fischer Benedikt Kessler Rosa Boano Matthew Collins Beatrice Demarchi |
| author_facet | Samantha Presslee Julie Wilson Jos Woolley Julia Best Douglas Russell Anita Radini Roman Fischer Benedikt Kessler Rosa Boano Matthew Collins Beatrice Demarchi |
| author_sort | Samantha Presslee |
| collection | DOAJ |
| description | Avian eggshell survives well in alkaline and neutral soils, but its potential as an archaeological resource remains largely unexplored, mainly due to difficulties in its identification. Here we exploit the release of novel bird genomes and, for the first time on eggshell, use MALDI-ToF (matrix-assisted laser desorption ionisation-time of flight) mass spectrometry in combination with peptide sequencing by LC-MS/MS. The eggshell proteome is revealed as unexpectedly complex, with 5755 proteins identified for a reference collection comprising 23 bird species. We determined 782 m/z markers useful for eggshell identification, 583 of which could be assigned to known eggshell peptide sequences. These were used to identify eggshell fragments recovered from a medieval site at Freeschool Lane, Leicester. We discuss the specificity of the peptide markers and highlight the importance of assessing the level of taxonomic identification achievable for archaeological interpretation. |
| first_indexed | 2024-12-22T04:47:05Z |
| format | Article |
| id | doaj.art-a1a938f30e9f4e048cba826569fb880f |
| institution | Directory Open Access Journal |
| issn | 2054-8923 |
| language | English |
| last_indexed | 2024-12-22T04:47:05Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Science and Technology of Archaeological Research |
| spelling | doaj.art-a1a938f30e9f4e048cba826569fb880f2022-12-21T18:38:34ZengTaylor & Francis GroupScience and Technology of Archaeological Research2054-89232017-01-0131899910.1080/20548923.2018.14243001424300The identification of archaeological eggshell using peptide markersSamantha Presslee0Julie Wilson1Jos Woolley2Julia Best3Douglas Russell4Anita Radini5Roman Fischer6Benedikt Kessler7Rosa Boano8Matthew Collins9Beatrice Demarchi10University of YorkUniversity of YorkIndependent researcherBournemouth UniversityNatural History MuseumUniversity of YorkTarget Discovery Institute, University of OxfordTarget Discovery Institute, University of OxfordUniversity of TurinUniversity of YorkUniversity of YorkAvian eggshell survives well in alkaline and neutral soils, but its potential as an archaeological resource remains largely unexplored, mainly due to difficulties in its identification. Here we exploit the release of novel bird genomes and, for the first time on eggshell, use MALDI-ToF (matrix-assisted laser desorption ionisation-time of flight) mass spectrometry in combination with peptide sequencing by LC-MS/MS. The eggshell proteome is revealed as unexpectedly complex, with 5755 proteins identified for a reference collection comprising 23 bird species. We determined 782 m/z markers useful for eggshell identification, 583 of which could be assigned to known eggshell peptide sequences. These were used to identify eggshell fragments recovered from a medieval site at Freeschool Lane, Leicester. We discuss the specificity of the peptide markers and highlight the importance of assessing the level of taxonomic identification achievable for archaeological interpretation.http://dx.doi.org/10.1080/20548923.2018.1424300Eggshellbirdszooarchaeologyproteomicsmass spectrometry (ZooMS) |
| spellingShingle | Samantha Presslee Julie Wilson Jos Woolley Julia Best Douglas Russell Anita Radini Roman Fischer Benedikt Kessler Rosa Boano Matthew Collins Beatrice Demarchi The identification of archaeological eggshell using peptide markers Science and Technology of Archaeological Research Eggshell birds zooarchaeology proteomics mass spectrometry (ZooMS) |
| title | The identification of archaeological eggshell using peptide markers |
| title_full | The identification of archaeological eggshell using peptide markers |
| title_fullStr | The identification of archaeological eggshell using peptide markers |
| title_full_unstemmed | The identification of archaeological eggshell using peptide markers |
| title_short | The identification of archaeological eggshell using peptide markers |
| title_sort | identification of archaeological eggshell using peptide markers |
| topic | Eggshell birds zooarchaeology proteomics mass spectrometry (ZooMS) |
| url | http://dx.doi.org/10.1080/20548923.2018.1424300 |
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