Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields
Foodborne pathogens are frequently associated with risks and outbreaks of many diseases; therefore, food safety and processing remain a priority to control and minimize these risks. In this work, nisin-loaded magnetic nanoparticles were used and activated by alternating 10 and 125 mT (peak to peak)...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/11/2/342 |
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| author | Vitalij Novickij Ramunė Stanevičienė Rūta Gruškienė Kazimieras Badokas Juliana Lukša Jolanta Sereikaitė Kęstutis Mažeika Nikolaj Višniakov Jurij Novickij Elena Servienė |
| author_facet | Vitalij Novickij Ramunė Stanevičienė Rūta Gruškienė Kazimieras Badokas Juliana Lukša Jolanta Sereikaitė Kęstutis Mažeika Nikolaj Višniakov Jurij Novickij Elena Servienė |
| author_sort | Vitalij Novickij |
| collection | DOAJ |
| description | Foodborne pathogens are frequently associated with risks and outbreaks of many diseases; therefore, food safety and processing remain a priority to control and minimize these risks. In this work, nisin-loaded magnetic nanoparticles were used and activated by alternating 10 and 125 mT (peak to peak) magnetic fields (AMFs) for biocontrol of bacteria <i>Listeria innocua,</i> a suitable model to study the inactivation of common foodborne pathogen <i>L. monocytogenes.</i> It was shown that <i>L. innocua</i> features high resistance to nisin-based bioactive nanoparticles, however, application of AMFs (15 and 30 min exposure) significantly potentiates the treatment resulting in considerable log reduction of viable cells. The morphological changes and the resulting cellular damage, which was induced by the synergistic treatment, was confirmed using scanning electron microscopy. The thermal effects were also estimated in the study. The results are useful for the development of new methods for treatment of the drug-resistant foodborne pathogens to minimize the risks of invasive infections. The proposed methodology is a contactless alternative to the currently established pulsed-electric field-based treatment in food processing. |
| first_indexed | 2024-03-09T03:18:09Z |
| format | Article |
| id | doaj.art-a1aee45a73f149c8abaf1a2ff6b79ca8 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T03:18:09Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-a1aee45a73f149c8abaf1a2ff6b79ca82023-12-03T15:15:46ZengMDPI AGNanomaterials2079-49912021-01-0111234210.3390/nano11020342Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic FieldsVitalij Novickij0Ramunė Stanevičienė1Rūta Gruškienė2Kazimieras Badokas3Juliana Lukša4Jolanta Sereikaitė5Kęstutis Mažeika6Nikolaj Višniakov7Jurij Novickij8Elena Servienė9Faculty of Electronics, Vilnius Gediminas Technical University, 03227 Vilnius, LithuaniaLaboratory of Genetics, Nature Research Centre, 08412 Vilnius, LithuaniaFaculty of Fundamental Sciences, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaInstitute of Photonics and Nanotechnology, Vilnius University, 10257 Vilnius, LithuaniaLaboratory of Genetics, Nature Research Centre, 08412 Vilnius, LithuaniaFaculty of Fundamental Sciences, Vilnius Gediminas Technical University, 10223 Vilnius, LithuaniaCenter for Physical Sciences and Technology, 02300 Vilnius, LithuaniaFaculty of Mechanics, Vilnius Gediminas Technical University, 03224 Vilnius, LithuaniaFaculty of Electronics, Vilnius Gediminas Technical University, 03227 Vilnius, LithuaniaLaboratory of Genetics, Nature Research Centre, 08412 Vilnius, LithuaniaFoodborne pathogens are frequently associated with risks and outbreaks of many diseases; therefore, food safety and processing remain a priority to control and minimize these risks. In this work, nisin-loaded magnetic nanoparticles were used and activated by alternating 10 and 125 mT (peak to peak) magnetic fields (AMFs) for biocontrol of bacteria <i>Listeria innocua,</i> a suitable model to study the inactivation of common foodborne pathogen <i>L. monocytogenes.</i> It was shown that <i>L. innocua</i> features high resistance to nisin-based bioactive nanoparticles, however, application of AMFs (15 and 30 min exposure) significantly potentiates the treatment resulting in considerable log reduction of viable cells. The morphological changes and the resulting cellular damage, which was induced by the synergistic treatment, was confirmed using scanning electron microscopy. The thermal effects were also estimated in the study. The results are useful for the development of new methods for treatment of the drug-resistant foodborne pathogens to minimize the risks of invasive infections. The proposed methodology is a contactless alternative to the currently established pulsed-electric field-based treatment in food processing.https://www.mdpi.com/2079-4991/11/2/342electromagnetic fieldsfood processingsterilizationnisin<i>L. innocua</i> |
| spellingShingle | Vitalij Novickij Ramunė Stanevičienė Rūta Gruškienė Kazimieras Badokas Juliana Lukša Jolanta Sereikaitė Kęstutis Mažeika Nikolaj Višniakov Jurij Novickij Elena Servienė Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields Nanomaterials electromagnetic fields food processing sterilization nisin <i>L. innocua</i> |
| title | Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields |
| title_full | Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields |
| title_fullStr | Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields |
| title_full_unstemmed | Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields |
| title_short | Inactivation of Bacteria Using Bioactive Nanoparticles and Alternating Magnetic Fields |
| title_sort | inactivation of bacteria using bioactive nanoparticles and alternating magnetic fields |
| topic | electromagnetic fields food processing sterilization nisin <i>L. innocua</i> |
| url | https://www.mdpi.com/2079-4991/11/2/342 |
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