Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?
Abstract: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were inf...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2018-05-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100238&lng=en&tlng=en |
| _version_ | 1818957348001021952 |
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| author | Rafaela Fernandes Zancan Lyz Cristina Furquim Canali Talita Tartari Flaviana Bombarda de Andrade Rodrigo Ricci Vivan Marco Antonio Hungaro Duarte |
| author_facet | Rafaela Fernandes Zancan Lyz Cristina Furquim Canali Talita Tartari Flaviana Bombarda de Andrade Rodrigo Ricci Vivan Marco Antonio Hungaro Duarte |
| author_sort | Rafaela Fernandes Zancan |
| collection | DOAJ |
| description | Abstract: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality. |
| first_indexed | 2024-12-20T11:08:25Z |
| format | Article |
| id | doaj.art-a1aff123be864779929163520165449f |
| institution | Directory Open Access Journal |
| issn | 1807-3107 |
| language | English |
| last_indexed | 2024-12-20T11:08:25Z |
| publishDate | 2018-05-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-a1aff123be864779929163520165449f2022-12-21T19:42:51ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1807-31072018-05-0132010.1590/1807-3107bor-2018.vol32.0046S1806-83242018000100238Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?Rafaela Fernandes ZancanLyz Cristina Furquim CanaliTalita TartariFlaviana Bombarda de AndradeRodrigo Ricci VivanMarco Antonio Hungaro DuarteAbstract: The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups: CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner: in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100238&lng=en&tlng=enEnterococcus faecalisCalcium HydroxideChlorhexidineAnti-Bacterial AgentsEndodontics |
| spellingShingle | Rafaela Fernandes Zancan Lyz Cristina Furquim Canali Talita Tartari Flaviana Bombarda de Andrade Rodrigo Ricci Vivan Marco Antonio Hungaro Duarte Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? Brazilian Oral Research Enterococcus faecalis Calcium Hydroxide Chlorhexidine Anti-Bacterial Agents Endodontics |
| title | Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? |
| title_full | Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? |
| title_fullStr | Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? |
| title_full_unstemmed | Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? |
| title_short | Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research? |
| title_sort | do different strains of e faecalis have the same behavior towards intracanal medications in in vitro research |
| topic | Enterococcus faecalis Calcium Hydroxide Chlorhexidine Anti-Bacterial Agents Endodontics |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242018000100238&lng=en&tlng=en |
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