Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland
The aim of the study was to determine the relationship between the chemical composition of eight commercial essential oils (EsO) (garlic, grapefruit, lemon grass, tea tree, thyme, verbena, cajeput, and <i>Litsea cubeba</i>) and their fungistatic activity in relation to four species of &l...
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MDPI AG
2020-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/2/292 |
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| author | Teresa Krzyśko-Łupicka Sławomir Sokół Anna Piekarska-Stachowiak |
| author_facet | Teresa Krzyśko-Łupicka Sławomir Sokół Anna Piekarska-Stachowiak |
| author_sort | Teresa Krzyśko-Łupicka |
| collection | DOAJ |
| description | The aim of the study was to determine the relationship between the chemical composition of eight commercial essential oils (EsO) (garlic, grapefruit, lemon grass, tea tree, thyme, verbena, cajeput, and <i>Litsea cubeba</i>) and their fungistatic activity in relation to four species of <i>Fusarium</i>: <i>F. avenaceum</i>, <i>F. culmorum</i>, <i>F. graminearum</i>, and <i>F. oxysporum</i>. The species identification of <i>Fusarium</i> isolates was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The determination of qualitative and quantitative chemical composition of the EsO was carried out using the gas chromatography−mass spectrometry (GC−MS) method. The fungistatic activity of EsO was assessed by using the method of poisoned substrates. The data were compiled in the STATISTICA 13.0 program. The chemical composition of the tested oils varied; the dominant fraction, except for grapefruit and garlic oils, were monoterpenoids. The greatest similarity to the action of the synthetic pesticide Funaben T was found in four oils, i.e., thyme, lemongrass, verbena, and <i>Litsea cubeba</i>. The studies showed that <i>F. oxysporum</i> and <i>F. avenaceum</i> were characterized by a higher resistance to low oil concentrations, and <i>F. culmorum</i> and <i>F. graminearum</i> by sensitivity. The fungicidal activity of two EsO-dominant monoterpenoids-thymol and citral—has been confirmed. |
| first_indexed | 2024-12-13T02:30:29Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-13T02:30:29Z |
| publishDate | 2020-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a1b195305ca54905bc385656e3501d512022-12-22T00:02:31ZengMDPI AGMolecules1420-30492020-01-0125229210.3390/molecules25020292molecules25020292Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern PolandTeresa Krzyśko-Łupicka0Sławomir Sokół1Anna Piekarska-Stachowiak2Institute of Environmental Engineering and Biotechnology, Faculty of Natural and Technical Sciences, University of Opole, Kominka 6A, 45-035 Opole, PolandInstitute of Biology, Faculty of Natural and Technical Sciences, University of Opole, Oleska 22, 45-052 Opole, PolandInstitute of Biology, Biotechnology an Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, Jagiellońska 28, 40-032 Katowice, PolandThe aim of the study was to determine the relationship between the chemical composition of eight commercial essential oils (EsO) (garlic, grapefruit, lemon grass, tea tree, thyme, verbena, cajeput, and <i>Litsea cubeba</i>) and their fungistatic activity in relation to four species of <i>Fusarium</i>: <i>F. avenaceum</i>, <i>F. culmorum</i>, <i>F. graminearum</i>, and <i>F. oxysporum</i>. The species identification of <i>Fusarium</i> isolates was confirmed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The determination of qualitative and quantitative chemical composition of the EsO was carried out using the gas chromatography−mass spectrometry (GC−MS) method. The fungistatic activity of EsO was assessed by using the method of poisoned substrates. The data were compiled in the STATISTICA 13.0 program. The chemical composition of the tested oils varied; the dominant fraction, except for grapefruit and garlic oils, were monoterpenoids. The greatest similarity to the action of the synthetic pesticide Funaben T was found in four oils, i.e., thyme, lemongrass, verbena, and <i>Litsea cubeba</i>. The studies showed that <i>F. oxysporum</i> and <i>F. avenaceum</i> were characterized by a higher resistance to low oil concentrations, and <i>F. culmorum</i> and <i>F. graminearum</i> by sensitivity. The fungicidal activity of two EsO-dominant monoterpenoids-thymol and citral—has been confirmed.https://www.mdpi.com/1420-3049/25/2/292fungistatic activity<i>fusarium</i><i>f. avenaceum</i><i>f. culmorum</i><i>f. graminearum</i><i>f. oxysporum</i>thymolcitral |
| spellingShingle | Teresa Krzyśko-Łupicka Sławomir Sokół Anna Piekarska-Stachowiak Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland Molecules fungistatic activity <i>fusarium</i> <i>f. avenaceum</i> <i>f. culmorum</i> <i>f. graminearum</i> <i>f. oxysporum</i> thymol citral |
| title | Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland |
| title_full | Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland |
| title_fullStr | Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland |
| title_full_unstemmed | Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland |
| title_short | Evaluation of Fungistatic Activity of Eight Selected Essential Oils on Four Heterogeneous <i>Fusarium</i> Isolates Obtained from Cereal Grains in Southern Poland |
| title_sort | evaluation of fungistatic activity of eight selected essential oils on four heterogeneous i fusarium i isolates obtained from cereal grains in southern poland |
| topic | fungistatic activity <i>fusarium</i> <i>f. avenaceum</i> <i>f. culmorum</i> <i>f. graminearum</i> <i>f. oxysporum</i> thymol citral |
| url | https://www.mdpi.com/1420-3049/25/2/292 |
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