Lentiviral reporter constructs for fluorescence tracking of the temporospatial pattern of Smad3 signaling
Canonical TGF-β is involved in cell differentiation, tissue maintenance, and wound healing, but also plays a central role in the pathogenesis of diseases such as cancer. Here we describe a lentivirus-based reporter vector system expressing green fluorescent protein (GFP) or red fluorescent protein (...
| Váldodahkkit: | , , , |
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| Materiálatiipa: | Artihkal |
| Giella: | English |
| Almmustuhtton: |
Taylor & Francis Group
2007-09-01
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| Ráidu: | BioTechniques |
| Liŋkkat: | https://www.future-science.com/doi/10.2144/000112549 |
| Čoahkkáigeassu: | Canonical TGF-β is involved in cell differentiation, tissue maintenance, and wound healing, but also plays a central role in the pathogenesis of diseases such as cancer. Here we describe a lentivirus-based reporter vector system expressing green fluorescent protein (GFP) or red fluorescent protein (RFP) under the control of a Smad3-responsive element (CAGA)12 that allows observation of the temporospatial pattern of endogeneous Smad3-mediated signaling on a cellular level. Use of this method will be valuable to identify cells with active Smad3 signaling and investigate the role of endogenous Smad3 signaling in complex systems such as co-cultures in vitro, or in tumors during tumor cell invasion and metastasis in vivo. |
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| ISSN: | 0736-6205 1940-9818 |