Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2
Leukocytes play an important role in vascular inflammation prior to atherosclerosis. In particular, monocyte adhesion and migration to the endothelium contribute to the development of vascular inflammation. Previously, we showed the importance of neutrophils and complement C5a in the early phase of...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2021-05-01
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| Series: | FEBS Open Bio |
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| Online Access: | https://doi.org/10.1002/2211-5463.13144 |
| _version_ | 1797374926398685184 |
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| author | Syed Masudur Rahman Dewan Mizuko Osaka Michiyo Deushi Masayuki Yoshida |
| author_facet | Syed Masudur Rahman Dewan Mizuko Osaka Michiyo Deushi Masayuki Yoshida |
| author_sort | Syed Masudur Rahman Dewan |
| collection | DOAJ |
| description | Leukocytes play an important role in vascular inflammation prior to atherosclerosis. In particular, monocyte adhesion and migration to the endothelium contribute to the development of vascular inflammation. Previously, we showed the importance of neutrophils and complement C5a in the early phase of vascular inflammation in mice fed a high‐fat diet. However, the relationship between monocytes and neutrophils is not well understood. In this study, we elucidated the involvement of neutrophils in the migration of monocytes. We observed that C5a induces CCL2 expression in neutrophil‐like dHL‐60 cells. To investigate the physiological significance of CCL2 secretion, we performed a chemotaxis assay. Interestingly, dHL‐60 culture supernatant in the presence of C5a enhanced the migration of THP‐1 in comparison with the absence of C5a. Furthermore, CCL2 expression and secretion significantly increased in C5a‐stimulated dHL‐60 through the phosphorylation of NF‐κB p65. Actin polymerization on THP‐1 was enhanced by the presence of C5a compared with the absence of C5a when stimulated by a dHL‐60‐cultured medium. These results suggest that crosstalk between neutrophils and monocytes via CCL2 may play an important role in vascular inflammation. |
| first_indexed | 2024-03-08T19:12:48Z |
| format | Article |
| id | doaj.art-a1b41c406bc24b3b861bbafeb7b7b1ad |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-03-08T19:12:48Z |
| publishDate | 2021-05-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-a1b41c406bc24b3b861bbafeb7b7b1ad2023-12-27T09:30:57ZengWileyFEBS Open Bio2211-54632021-05-011151374138110.1002/2211-5463.13144Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2Syed Masudur Rahman Dewan0Mizuko Osaka1Michiyo Deushi2Masayuki Yoshida3Department of Life Sciences and Bioethics Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University JapanDepartment of Life Sciences and Bioethics Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University JapanDepartment of Life Sciences and Bioethics Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University JapanDepartment of Life Sciences and Bioethics Graduate School of Medical and Dental Sciences Tokyo Medical and Dental University JapanLeukocytes play an important role in vascular inflammation prior to atherosclerosis. In particular, monocyte adhesion and migration to the endothelium contribute to the development of vascular inflammation. Previously, we showed the importance of neutrophils and complement C5a in the early phase of vascular inflammation in mice fed a high‐fat diet. However, the relationship between monocytes and neutrophils is not well understood. In this study, we elucidated the involvement of neutrophils in the migration of monocytes. We observed that C5a induces CCL2 expression in neutrophil‐like dHL‐60 cells. To investigate the physiological significance of CCL2 secretion, we performed a chemotaxis assay. Interestingly, dHL‐60 culture supernatant in the presence of C5a enhanced the migration of THP‐1 in comparison with the absence of C5a. Furthermore, CCL2 expression and secretion significantly increased in C5a‐stimulated dHL‐60 through the phosphorylation of NF‐κB p65. Actin polymerization on THP‐1 was enhanced by the presence of C5a compared with the absence of C5a when stimulated by a dHL‐60‐cultured medium. These results suggest that crosstalk between neutrophils and monocytes via CCL2 may play an important role in vascular inflammation.https://doi.org/10.1002/2211-5463.13144C5aCCL2chemotaxisneutrophil‐like dHL‐60monocytic leukocyte THP‐1NF‐κB p65 |
| spellingShingle | Syed Masudur Rahman Dewan Mizuko Osaka Michiyo Deushi Masayuki Yoshida Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 FEBS Open Bio C5a CCL2 chemotaxis neutrophil‐like dHL‐60 monocytic leukocyte THP‐1 NF‐κB p65 |
| title | Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 |
| title_full | Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 |
| title_fullStr | Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 |
| title_full_unstemmed | Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 |
| title_short | Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2 |
| title_sort | complement c5a triggered differentiated hl 60 stimulates migration of thp 1 monocytic leukocytes via secretion of ccl2 |
| topic | C5a CCL2 chemotaxis neutrophil‐like dHL‐60 monocytic leukocyte THP‐1 NF‐κB p65 |
| url | https://doi.org/10.1002/2211-5463.13144 |
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