Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber
Cucumber is an important vegetable crop grown worldwide and highly sensitive to prevailing temperature condition. The physiological, biochemical and molecular basis of high temperature stress tolerance is poorly understood in this model vegetable crop. In the present study, a set of genotypes with c...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1128928/full |
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| author | Dhananjay A. Hongal Dhandapani Raju Sudhir Kumar Akshay Talukdar Anjan Das Khushboo Kumari Prasanta K. Dash Viswanathan Chinnusamy Anilabha Das Munshi Tusar Kanti Behera Tusar Kanti Behera Shyam Sundar Dey |
| author_facet | Dhananjay A. Hongal Dhandapani Raju Sudhir Kumar Akshay Talukdar Anjan Das Khushboo Kumari Prasanta K. Dash Viswanathan Chinnusamy Anilabha Das Munshi Tusar Kanti Behera Tusar Kanti Behera Shyam Sundar Dey |
| author_sort | Dhananjay A. Hongal |
| collection | DOAJ |
| description | Cucumber is an important vegetable crop grown worldwide and highly sensitive to prevailing temperature condition. The physiological, biochemical and molecular basis of high temperature stress tolerance is poorly understood in this model vegetable crop. In the present study, a set of genotypes with contrasting response under two different temperature stress (35/30°C and 40/35°C) were evaluated for important physiological and biochemical traits. Besides, expression of the important heat shock proteins (HSPs), aquaporins (AQPs), photosynthesis related genes was conducted in two selected contrasting genotypes at different stress conditions. It was established that tolerant genotypes were able to maintain high chlorophyll retention, stable membrane stability index, higher retention of water content, stability in net photosynthesis, high stomatal conductance and transpiration in combination with less canopy temperatures under high temperature stress conditions compared to susceptible genotypes and were considered as the key physiological traits associated with heat tolerance in cucumber. Accumulation of biochemicals like proline, protein and antioxidants like SOD, catalase and peroxidase was the underlying biochemical mechanisms for high temperature tolerance. Upregulation of photosynthesis related genes, signal transduction genes and heat responsive genes (HSPs) in tolerant genotypes indicate the molecular network associated with heat tolerance in cucumber. Among the HSPs, higher accumulation of HSP70 and HSP90 were recorded in the tolerant genotype, WBC-13 under heat stress condition indicating their critical role. Besides, Rubisco S, Rubisco L and CsTIP1b were upregulated in the tolerant genotypes under heat stress condition. Therefore, the HSPs in combination with photosynthetic and aquaporin genes were the underlying important molecular network associated with heat stress tolerance in cucumber. The findings of the present study also indicated negative feedback of G-protein alpha unit and oxygen evolving complex in relation to heat stress tolerance in cucumber. These results indicate that the thermotolerant cucumber genotypes enhanced physio-biochemical and molecular adaptation under high-temperature stress condition. This study provides foundation to design climate smart genotypes in cucumber through integration of favorable physio-biochemical traits and understanding the detailed molecular network associated with heat stress tolerance in cucumber. |
| first_indexed | 2024-04-10T09:02:26Z |
| format | Article |
| id | doaj.art-a1b433b566344678be37b21a0572524e |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-10T09:02:26Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a1b433b566344678be37b21a0572524e2023-02-21T11:46:08ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-02-011410.3389/fpls.2023.11289281128928Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumberDhananjay A. Hongal0Dhandapani Raju1Sudhir Kumar2Akshay Talukdar3Anjan Das4Khushboo Kumari5Prasanta K. Dash6Viswanathan Chinnusamy7Anilabha Das Munshi8Tusar Kanti Behera9Tusar Kanti Behera10Shyam Sundar Dey11Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Genetics, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-National Institute for Plant Biotechnology, New Delhi, IndiaDivision of Plant Physiology, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaDivision of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaICAR-Indian Institute of Vegetable Research, Varanasi, IndiaDivision of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, IndiaCucumber is an important vegetable crop grown worldwide and highly sensitive to prevailing temperature condition. The physiological, biochemical and molecular basis of high temperature stress tolerance is poorly understood in this model vegetable crop. In the present study, a set of genotypes with contrasting response under two different temperature stress (35/30°C and 40/35°C) were evaluated for important physiological and biochemical traits. Besides, expression of the important heat shock proteins (HSPs), aquaporins (AQPs), photosynthesis related genes was conducted in two selected contrasting genotypes at different stress conditions. It was established that tolerant genotypes were able to maintain high chlorophyll retention, stable membrane stability index, higher retention of water content, stability in net photosynthesis, high stomatal conductance and transpiration in combination with less canopy temperatures under high temperature stress conditions compared to susceptible genotypes and were considered as the key physiological traits associated with heat tolerance in cucumber. Accumulation of biochemicals like proline, protein and antioxidants like SOD, catalase and peroxidase was the underlying biochemical mechanisms for high temperature tolerance. Upregulation of photosynthesis related genes, signal transduction genes and heat responsive genes (HSPs) in tolerant genotypes indicate the molecular network associated with heat tolerance in cucumber. Among the HSPs, higher accumulation of HSP70 and HSP90 were recorded in the tolerant genotype, WBC-13 under heat stress condition indicating their critical role. Besides, Rubisco S, Rubisco L and CsTIP1b were upregulated in the tolerant genotypes under heat stress condition. Therefore, the HSPs in combination with photosynthetic and aquaporin genes were the underlying important molecular network associated with heat stress tolerance in cucumber. The findings of the present study also indicated negative feedback of G-protein alpha unit and oxygen evolving complex in relation to heat stress tolerance in cucumber. These results indicate that the thermotolerant cucumber genotypes enhanced physio-biochemical and molecular adaptation under high-temperature stress condition. This study provides foundation to design climate smart genotypes in cucumber through integration of favorable physio-biochemical traits and understanding the detailed molecular network associated with heat stress tolerance in cucumber.https://www.frontiersin.org/articles/10.3389/fpls.2023.1128928/fullcucumberheat stressphysiological and biochemical traitsantioxidant enzymesRT-PCRHSPs |
| spellingShingle | Dhananjay A. Hongal Dhandapani Raju Sudhir Kumar Akshay Talukdar Anjan Das Khushboo Kumari Prasanta K. Dash Viswanathan Chinnusamy Anilabha Das Munshi Tusar Kanti Behera Tusar Kanti Behera Shyam Sundar Dey Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber Frontiers in Plant Science cucumber heat stress physiological and biochemical traits antioxidant enzymes RT-PCR HSPs |
| title | Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| title_full | Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| title_fullStr | Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| title_full_unstemmed | Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| title_short | Elucidating the role of key physio-biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| title_sort | elucidating the role of key physio biochemical traits and molecular network conferring heat stress tolerance in cucumber |
| topic | cucumber heat stress physiological and biochemical traits antioxidant enzymes RT-PCR HSPs |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1128928/full |
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