Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise
Background Ascorbic acid is a water-soluble chain breaking antioxidant. It scavenges free radicals and reactive oxygen species (ROS), which are produced during metabolic pathways. Exercise can produce an imbalance between ROS and antioxidants, leading to oxidative stress-related tissue damages. This...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Journal of the International Society of Sports Nutrition |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1186/s12970-019-0269-8 |
| _version_ | 1797685030769655808 |
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| author | Manita Yimcharoen Suwatsin Kittikunnathum Chawannut Suknikorn Wichuda Nak-on Petcharee Yeethong Tracy G. Anthony Piyawan Bunpo |
| author_facet | Manita Yimcharoen Suwatsin Kittikunnathum Chawannut Suknikorn Wichuda Nak-on Petcharee Yeethong Tracy G. Anthony Piyawan Bunpo |
| author_sort | Manita Yimcharoen |
| collection | DOAJ |
| description | Background Ascorbic acid is a water-soluble chain breaking antioxidant. It scavenges free radicals and reactive oxygen species (ROS), which are produced during metabolic pathways. Exercise can produce an imbalance between ROS and antioxidants, leading to oxidative stress-related tissue damages. This study was designed to determine the effects of ascorbic acid supplementation on circulating biomarkers of oxidative stress and muscle damage following a single bout of exercise. Methods In a crossover design with a 1 wk. wash-out period, 19 healthy women performed 30 min moderate-intensity cycling after ingesting 1000 mg of ascorbic acid (AA) or placebo. Blood samples were taken immediately before, immediately after and 30 min post-exercise to determine plasma albumin, total protein, glucose, oxidative stress and muscle damage markers. Results Plasma albumin and total protein levels increased immediately after exercise in placebo alongside slight reductions in glucose (p = 0.001). These effects were absent in AA cohort. Ferric reducing ability of plasma and vitamin C levels in AA cohort significantly increased after exercise (p < 0.05). Superoxide dismutase activity was significantly elevated after exercise (p = 0.002) in placebo but not AA. Plasma malondialdehyde did not change after exercise in placebo but was significantly decreased in AA (p < 0.05). The exercise protocol promoted slight muscle damage, reflected in significant increases in total creatine kinase in all subjects after exercise. On the other hand, plasma C-reactive protein and lactate dehydrogenase remained unchanged. Conclusion Supplementation with ascorbic acid prior exercise improves antioxidant power but does not prevent muscle damage. |
| first_indexed | 2024-03-12T00:38:28Z |
| format | Article |
| id | doaj.art-a1b5a7edb950424c867d052beed2a197 |
| institution | Directory Open Access Journal |
| issn | 1550-2783 |
| language | English |
| last_indexed | 2024-03-12T00:38:28Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of the International Society of Sports Nutrition |
| spelling | doaj.art-a1b5a7edb950424c867d052beed2a1972023-09-15T09:19:30ZengTaylor & Francis GroupJournal of the International Society of Sports Nutrition1550-27832019-01-0116110.1186/s12970-019-0269-812130080Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exerciseManita Yimcharoen0Suwatsin Kittikunnathum1Chawannut Suknikorn2Wichuda Nak-on3Petcharee Yeethong4Tracy G. Anthony5Piyawan Bunpo6Department of Medical Technology, Faculty of Associated Medical SciencesDepartment of Medical Technology, Faculty of Associated Medical SciencesDepartment of Medical Technology, Faculty of Associated Medical SciencesDepartment of Medical Technology, Faculty of Associated Medical SciencesDepartment of Medical Technology, Faculty of Associated Medical SciencesDepartment of Nutritional SciencesDepartment of Medical Technology, Faculty of Associated Medical SciencesBackground Ascorbic acid is a water-soluble chain breaking antioxidant. It scavenges free radicals and reactive oxygen species (ROS), which are produced during metabolic pathways. Exercise can produce an imbalance between ROS and antioxidants, leading to oxidative stress-related tissue damages. This study was designed to determine the effects of ascorbic acid supplementation on circulating biomarkers of oxidative stress and muscle damage following a single bout of exercise. Methods In a crossover design with a 1 wk. wash-out period, 19 healthy women performed 30 min moderate-intensity cycling after ingesting 1000 mg of ascorbic acid (AA) or placebo. Blood samples were taken immediately before, immediately after and 30 min post-exercise to determine plasma albumin, total protein, glucose, oxidative stress and muscle damage markers. Results Plasma albumin and total protein levels increased immediately after exercise in placebo alongside slight reductions in glucose (p = 0.001). These effects were absent in AA cohort. Ferric reducing ability of plasma and vitamin C levels in AA cohort significantly increased after exercise (p < 0.05). Superoxide dismutase activity was significantly elevated after exercise (p = 0.002) in placebo but not AA. Plasma malondialdehyde did not change after exercise in placebo but was significantly decreased in AA (p < 0.05). The exercise protocol promoted slight muscle damage, reflected in significant increases in total creatine kinase in all subjects after exercise. On the other hand, plasma C-reactive protein and lactate dehydrogenase remained unchanged. Conclusion Supplementation with ascorbic acid prior exercise improves antioxidant power but does not prevent muscle damage.http://dx.doi.org/10.1186/s12970-019-0269-8exercisevitamin coxidative stress markersglucoseinflammationmuscle damage |
| spellingShingle | Manita Yimcharoen Suwatsin Kittikunnathum Chawannut Suknikorn Wichuda Nak-on Petcharee Yeethong Tracy G. Anthony Piyawan Bunpo Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise Journal of the International Society of Sports Nutrition exercise vitamin c oxidative stress markers glucose inflammation muscle damage |
| title | Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| title_full | Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| title_fullStr | Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| title_full_unstemmed | Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| title_short | Effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| title_sort | effects of ascorbic acid supplementation on oxidative stress markers in healthy women following a single bout of exercise |
| topic | exercise vitamin c oxidative stress markers glucose inflammation muscle damage |
| url | http://dx.doi.org/10.1186/s12970-019-0269-8 |
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