Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays
Traumatic brain injury (TBI) is a primary global health concern and one of the most common causes of neurological impairments in people under 50. Mild TBI (mTBI) accounts for the majority of TBI cases. Anxiety is the most common complaint after mTBI in humans. This study aims to evaluate behavioral...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Behavioral Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1140724/full |
| _version_ | 1797866043984576512 |
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| author | Liron Tseitlin Bar Richmond-Hacham Adi Vita Shaul Schreiber Shaul Schreiber Shaul Schreiber Chaim G. Pick Chaim G. Pick Chaim G. Pick Chaim G. Pick Lior Bikovski Lior Bikovski |
| author_facet | Liron Tseitlin Bar Richmond-Hacham Adi Vita Shaul Schreiber Shaul Schreiber Shaul Schreiber Chaim G. Pick Chaim G. Pick Chaim G. Pick Chaim G. Pick Lior Bikovski Lior Bikovski |
| author_sort | Liron Tseitlin |
| collection | DOAJ |
| description | Traumatic brain injury (TBI) is a primary global health concern and one of the most common causes of neurological impairments in people under 50. Mild TBI (mTBI) accounts for the majority of TBI cases. Anxiety is the most common complaint after mTBI in humans. This study aims to evaluate behavioral tests designed to assess anxiety-like phenotypes in a mice model of mTBI. ICR mice underwent mTBI using the weight-drop model. Seven days post-injury, mice were subjected to one of five different behavioral tests: Elevated Plus Maze (EPM), Open Field apparatus (OF), Marble Burying test (MBT), Light Dark Box (LDB), and the Light Spot test within the PhenoTyper home cage (LS). In the EPM and OF tests, there were no significant differences between the groups. During the 30-min test period of the MBT, mTBI mice buried significantly more marbles than control mice. In the LDB, mTBI mice spent significantly less time on the far side of the arena than control mice. In addition, the time it took for mTBI mice to get to the far side of the arena was significantly longer compared to controls. Results of LS show significant within-group mean differences for total distance traveled for mTBI mice but not for the control. Furthermore, injured mice moved significantly more than control mice. According to the results, the anxiety traits exhibited by mTBI mice depend upon the time of exposure to the aversive stimulus, the apparatus, and the properties of the stressors used. Therefore, the characterization of anxiety-like behavior in mTBI mice is more complicated than was initially suggested. Based on our findings, we recommend incorporating a variety of stressors and test session lengths when assessing anxiety-like behavior in experimental models of mTBI. |
| first_indexed | 2024-04-09T23:18:45Z |
| format | Article |
| id | doaj.art-a1b7e341cb3643a0bae0a56b5fb9882f |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-04-09T23:18:45Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-a1b7e341cb3643a0bae0a56b5fb9882f2023-03-22T05:15:49ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532023-03-011710.3389/fnbeh.2023.11407241140724Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assaysLiron Tseitlin0Bar Richmond-Hacham1Adi Vita2Shaul Schreiber3Shaul Schreiber4Shaul Schreiber5Chaim G. Pick6Chaim G. Pick7Chaim G. Pick8Chaim G. Pick9Lior Bikovski10Lior Bikovski11Department of Anatomy and Anthropology, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, IsraelDepartment of Anatomy and Anthropology, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, IsraelDepartment of Anatomy and Anthropology, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, IsraelDepartment of Psychiatry, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelDr. Miriam and Sheldon G. Adelson Clinic for Drug Abuse Treatment and Research, Tel Aviv Sourasky Medical Center, Tel Aviv, IsraelSackler Faculty of Medicine and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, IsraelDepartment of Anatomy and Anthropology, Tel Aviv University Sackler Faculty of Medicine, Tel Aviv, IsraelSackler Faculty of Medicine and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, IsraelSylvan Adams Sports Institute, Tel Aviv University, Tel Aviv, IsraelDr. Miriam and Sheldon G. Adelson Chair and Center for the Biology of Addictive Diseases, Tel-Aviv University, Tel-Aviv, IsraelMyers Neuro-Behavioral Core Facility, Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, IsraelSchool of Behavioral Sciences, Netanya Academic College, Netanya, IsraelTraumatic brain injury (TBI) is a primary global health concern and one of the most common causes of neurological impairments in people under 50. Mild TBI (mTBI) accounts for the majority of TBI cases. Anxiety is the most common complaint after mTBI in humans. This study aims to evaluate behavioral tests designed to assess anxiety-like phenotypes in a mice model of mTBI. ICR mice underwent mTBI using the weight-drop model. Seven days post-injury, mice were subjected to one of five different behavioral tests: Elevated Plus Maze (EPM), Open Field apparatus (OF), Marble Burying test (MBT), Light Dark Box (LDB), and the Light Spot test within the PhenoTyper home cage (LS). In the EPM and OF tests, there were no significant differences between the groups. During the 30-min test period of the MBT, mTBI mice buried significantly more marbles than control mice. In the LDB, mTBI mice spent significantly less time on the far side of the arena than control mice. In addition, the time it took for mTBI mice to get to the far side of the arena was significantly longer compared to controls. Results of LS show significant within-group mean differences for total distance traveled for mTBI mice but not for the control. Furthermore, injured mice moved significantly more than control mice. According to the results, the anxiety traits exhibited by mTBI mice depend upon the time of exposure to the aversive stimulus, the apparatus, and the properties of the stressors used. Therefore, the characterization of anxiety-like behavior in mTBI mice is more complicated than was initially suggested. Based on our findings, we recommend incorporating a variety of stressors and test session lengths when assessing anxiety-like behavior in experimental models of mTBI.https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1140724/fullTBIanxietybehaviorelevated plus mazeopen fieldmarble burying test |
| spellingShingle | Liron Tseitlin Bar Richmond-Hacham Adi Vita Shaul Schreiber Shaul Schreiber Shaul Schreiber Chaim G. Pick Chaim G. Pick Chaim G. Pick Chaim G. Pick Lior Bikovski Lior Bikovski Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays Frontiers in Behavioral Neuroscience TBI anxiety behavior elevated plus maze open field marble burying test |
| title | Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays |
| title_full | Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays |
| title_fullStr | Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays |
| title_full_unstemmed | Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays |
| title_short | Measuring anxiety-like behavior in a mouse model of mTBI: Assessment in standard and home cage assays |
| title_sort | measuring anxiety like behavior in a mouse model of mtbi assessment in standard and home cage assays |
| topic | TBI anxiety behavior elevated plus maze open field marble burying test |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1140724/full |
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