Protein sequences bound to mineral surfaces persist into deep time
Proteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2016-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/17092 |
| _version_ | 1828376178948308992 |
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| author | Beatrice Demarchi Shaun Hall Teresa Roncal-Herrero Colin L Freeman Jos Woolley Molly K Crisp Julie Wilson Anna Fotakis Roman Fischer Benedikt M Kessler Rosa Rakownikow Jersie-Christensen Jesper V Olsen James Haile Jessica Thomas Curtis W Marean John Parkington Samantha Presslee Julia Lee-Thorp Peter Ditchfield Jacqueline F Hamilton Martyn W Ward Chunting Michelle Wang Marvin D Shaw Terry Harrison Manuel Domínguez-Rodrigo Ross DE MacPhee Amandus Kwekason Michaela Ecker Liora Kolska Horwitz Michael Chazan Roland Kröger Jane Thomas-Oates John H Harding Enrico Cappellini Kirsty Penkman Matthew J Collins |
| author_facet | Beatrice Demarchi Shaun Hall Teresa Roncal-Herrero Colin L Freeman Jos Woolley Molly K Crisp Julie Wilson Anna Fotakis Roman Fischer Benedikt M Kessler Rosa Rakownikow Jersie-Christensen Jesper V Olsen James Haile Jessica Thomas Curtis W Marean John Parkington Samantha Presslee Julia Lee-Thorp Peter Ditchfield Jacqueline F Hamilton Martyn W Ward Chunting Michelle Wang Marvin D Shaw Terry Harrison Manuel Domínguez-Rodrigo Ross DE MacPhee Amandus Kwekason Michaela Ecker Liora Kolska Horwitz Michael Chazan Roland Kröger Jane Thomas-Oates John H Harding Enrico Cappellini Kirsty Penkman Matthew J Collins |
| author_sort | Beatrice Demarchi |
| collection | DOAJ |
| description | Proteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli (3.8 Ma) and Olduvai Gorge (1.3 Ma) in Tanzania. By tracking protein diagenesis back in time we find consistent patterns of preservation, demonstrating authenticity of the surviving sequences. Molecular dynamics simulations of struthiocalcin-1 and -2, the dominant proteins within the eggshell, reveal that distinct domains bind to the mineral surface. It is the domain with the strongest calculated binding energy to the calcite surface that is selectively preserved. Thermal age calculations demonstrate that the Laetoli and Olduvai peptides are 50 times older than any previously authenticated sequence (equivalent to ~16 Ma at a constant 10°C). |
| first_indexed | 2024-04-14T07:55:56Z |
| format | Article |
| id | doaj.art-a1ba7c012aeb465cb1b77e045d57372c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:55:56Z |
| publishDate | 2016-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a1ba7c012aeb465cb1b77e045d57372c2022-12-22T02:05:03ZengeLife Sciences Publications LtdeLife2050-084X2016-09-01510.7554/eLife.17092Protein sequences bound to mineral surfaces persist into deep timeBeatrice Demarchi0https://orcid.org/0000-0002-8398-4409Shaun Hall1Teresa Roncal-Herrero2Colin L Freeman3Jos Woolley4Molly K Crisp5Julie Wilson6Anna Fotakis7Roman Fischer8Benedikt M Kessler9Rosa Rakownikow Jersie-Christensen10Jesper V Olsen11James Haile12Jessica Thomas13Curtis W Marean14John Parkington15Samantha Presslee16Julia Lee-Thorp17Peter Ditchfield18Jacqueline F Hamilton19Martyn W Ward20Chunting Michelle Wang21Marvin D Shaw22Terry Harrison23Manuel Domínguez-Rodrigo24Ross DE MacPhee25Amandus Kwekason26Michaela Ecker27https://orcid.org/0000-0001-9581-1882Liora Kolska Horwitz28Michael Chazan29Roland Kröger30Jane Thomas-Oates31John H Harding32Enrico Cappellini33Kirsty Penkman34Matthew J Collins35BioArCh, Department of Archaeology, University of York, York, United KingdomDepartment of Material Science and Engineering, University of Sheffield, Sheffield, United KingdomDepartment of Physics, University of York, York, United KingdomDepartment of Material Science and Engineering, University of Sheffield, Sheffield, United KingdomBioArCh, Department of Archaeology, University of York, York, United KingdomDepartment of Chemistry, University of York, York, United KingdomDepartment of Chemistry, University of York, York, United Kingdom; Department of Mathematics, University of York, York, United KingdomCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkAdvanced Proteomics Facility, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomAdvanced Proteomics Facility, Target Discovery Institute, Nuffield Department of Medicine, University of Oxford, Oxford, United KingdomNovo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, DenmarkNovo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, Copenhagen, DenmarkResearch Laboratory for Archaeology and the History of Art, University of Oxford, Oxford, United KingdomCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, Denmark; Molecular Ecology and Fisheries Genetics Laboratory, School of Biological Sciences, Bangor University, Bangor, United KingdomInstitute of Human Origins, SHESC, Arizona State University, Tempe, United States; Centre for Coastal Palaeoscience, Nelson Mandela Metropolitan University, Port Elizabeth, South AfricaDepartment of Archaeology, University of Cape Town, Cape Town, South AfricaBioArCh, Department of Archaeology, University of York, York, United KingdomResearch Laboratory for Archaeology and the History of Art, University of Oxford, Oxford, United KingdomResearch Laboratory for Archaeology and the History of Art, University of Oxford, Oxford, United KingdomWolfson Atmospheric Chemistry Laboratories, Department of Chemistry, University of York, York, United KingdomWolfson Atmospheric Chemistry Laboratories, Department of Chemistry, University of York, York, United KingdomWolfson Atmospheric Chemistry Laboratories, Department of Chemistry, University of York, York, United KingdomWolfson Atmospheric Chemistry Laboratories, Department of Chemistry, University of York, York, United KingdomCenter for the Study of Human Origins, Department of Anthropology, New York University, New York, United StatesDepartment of Prehistory, Complutense University of Madrid, Madrid, SpainDepartment of Mammalogy, American Museum of Natural History, New York, United StatesNational Museum of Tanzania, Dar es Salaam, TanzaniaResearch Laboratory for Archaeology and the History of Art, University of Oxford, Oxford, United KingdomNational Natural History Collections, Faculty of Life Sciences, The Hebrew University, Jerusalem, IsraelDepartment of Anthropology, University of Toronto, Toronto, Canada; Evolutionary Studies Institute, University of the Witwatersrand, Braamfontein, South AfricaDepartment of Physics, University of York, York, United KingdomDepartment of Chemistry, University of York, York, United Kingdom; Centre of Excellence in Mass Spectrometry, University of York, New York, United StatesDepartment of Material Science and Engineering, University of Sheffield, Sheffield, United KingdomCentre for GeoGenetics, Natural History Museum of Denmark, University of Copenhagen, Copenhagen, DenmarkDepartment of Chemistry, University of York, York, United KingdomBioArCh, Department of Archaeology, University of York, York, United KingdomProteins persist longer in the fossil record than DNA, but the longevity, survival mechanisms and substrates remain contested. Here, we demonstrate the role of mineral binding in preserving the protein sequence in ostrich (Struthionidae) eggshell, including from the palaeontological sites of Laetoli (3.8 Ma) and Olduvai Gorge (1.3 Ma) in Tanzania. By tracking protein diagenesis back in time we find consistent patterns of preservation, demonstrating authenticity of the surviving sequences. Molecular dynamics simulations of struthiocalcin-1 and -2, the dominant proteins within the eggshell, reveal that distinct domains bind to the mineral surface. It is the domain with the strongest calculated binding energy to the calcite surface that is selectively preserved. Thermal age calculations demonstrate that the Laetoli and Olduvai peptides are 50 times older than any previously authenticated sequence (equivalent to ~16 Ma at a constant 10°C).https://elifesciences.org/articles/17092paleoproteomicseggshellmolecular dynamicspaleontologybiomineralizationStruthio camelus |
| spellingShingle | Beatrice Demarchi Shaun Hall Teresa Roncal-Herrero Colin L Freeman Jos Woolley Molly K Crisp Julie Wilson Anna Fotakis Roman Fischer Benedikt M Kessler Rosa Rakownikow Jersie-Christensen Jesper V Olsen James Haile Jessica Thomas Curtis W Marean John Parkington Samantha Presslee Julia Lee-Thorp Peter Ditchfield Jacqueline F Hamilton Martyn W Ward Chunting Michelle Wang Marvin D Shaw Terry Harrison Manuel Domínguez-Rodrigo Ross DE MacPhee Amandus Kwekason Michaela Ecker Liora Kolska Horwitz Michael Chazan Roland Kröger Jane Thomas-Oates John H Harding Enrico Cappellini Kirsty Penkman Matthew J Collins Protein sequences bound to mineral surfaces persist into deep time eLife paleoproteomics eggshell molecular dynamics paleontology biomineralization Struthio camelus |
| title | Protein sequences bound to mineral surfaces persist into deep time |
| title_full | Protein sequences bound to mineral surfaces persist into deep time |
| title_fullStr | Protein sequences bound to mineral surfaces persist into deep time |
| title_full_unstemmed | Protein sequences bound to mineral surfaces persist into deep time |
| title_short | Protein sequences bound to mineral surfaces persist into deep time |
| title_sort | protein sequences bound to mineral surfaces persist into deep time |
| topic | paleoproteomics eggshell molecular dynamics paleontology biomineralization Struthio camelus |
| url | https://elifesciences.org/articles/17092 |
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