Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae)
<i>Hyssopus officinalis</i> L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extrac...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/4/711 |
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| author | Tijana Mićović Dijana Topalović Lada Živković Biljana Spremo-Potparević Vladimir Jakovljević Sanja Matić Suzana Popović Dejan Baskić Danijela Stešević Stevan Samardžić Danilo Stojanović Zoran Maksimović |
| author_facet | Tijana Mićović Dijana Topalović Lada Živković Biljana Spremo-Potparević Vladimir Jakovljević Sanja Matić Suzana Popović Dejan Baskić Danijela Stešević Stevan Samardžić Danilo Stojanović Zoran Maksimović |
| author_sort | Tijana Mićović |
| collection | DOAJ |
| description | <i>Hyssopus officinalis</i> L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC<sub>50</sub> = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe<sup>2+</sup>/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable <i>H</i>. <i>officinalis</i> medicinal potential, which calls for further investigation. |
| first_indexed | 2024-03-10T12:34:04Z |
| format | Article |
| id | doaj.art-a1be612f4d1e42678f6492caa1dd480e |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-10T12:34:04Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
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| series | Plants |
| spelling | doaj.art-a1be612f4d1e42678f6492caa1dd480e2023-11-21T14:27:59ZengMDPI AGPlants2223-77472021-04-0110471110.3390/plants10040711Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae)Tijana Mićović0Dijana Topalović1Lada Živković2Biljana Spremo-Potparević3Vladimir Jakovljević4Sanja Matić5Suzana Popović6Dejan Baskić7Danijela Stešević8Stevan Samardžić9Danilo Stojanović10Zoran Maksimović11Institute for Medicines and Medical Devices of Montenegro, Bulevar Ivana Crnojevića 64a, 81000 Podgorica, MontenegroDepartment of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, SerbiaDepartment of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, SerbiaDepartment of Pathobiology, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, SerbiaDepartment of Physiology, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, SerbiaDepartment of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, SerbiaDepartment of Microbiology and Immunology, Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, SerbiaDepartment of Microbiology and Immunology, Center for Molecular Medicine and Stem Cell Research, Faculty of Medical Sciences, University of Kragujevac, Svetozara Markovića 69, 34000 Kragujevac, SerbiaFaculty of Natural Sciences and Mathematics, University of Montenegro, Džordža Vašingtona bb, 81000 Podgorica, MontenegroDepartment of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, SerbiaDepartment of Botany, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, SerbiaDepartment of Pharmacognosy, Faculty of Pharmacy, University of Belgrade, Vojvode Stepe 450, 11000 Belgrade, Serbia<i>Hyssopus officinalis</i> L. is a well-known aromatic plant used in traditional medicine and the food and cosmetics industry. The aim of this study is to assess the antioxidant, genotoxic, antigenotoxic and cytotoxic properties of characterized hyssop essential oils and methanol extracts. Chemical composition was analyzed by gas chromatography - mass spectrometry (GC-MS) and liquid chromatography with diode array detection and mass spectrometry (LC-DAD-MS), respectively. Antioxidant activity was examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing/antioxidant power (FRAP) tests; genotoxic and antigenotoxic activity were examined by the comet assay, while cytotoxicity was evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide dye (MTT) test against tumor cell lines (SW480, MDA-MB 231, HeLa) and non-transformed human lung fibroblast cell lines (MRC-5). The essential oils were rich in monoterpene hydrocarbons (e.g., limonene; 7.99–23.81%), oxygenated monoterpenes (1,8-cineole; 38.19–67.1%) and phenylpropanoids (methyl eugenol; 0.00–28.33%). In methanol extracts, the most abundant phenolics were chlorogenic and rosmarinic acid (23.35–33.46 and 3.53–17.98 mg/g, respectively). Methanol extracts expressed moderate to weak antioxidant activity (DPPH IC<sub>50</sub> = 56.04–199.89 µg/mL, FRAP = 0.667–0.959 mmol Fe<sup>2+</sup>/g). Hyssop preparations significantly reduced DNA damage in human whole blood cells, induced by pretreatment with hydrogen peroxide. Methanol extracts exhibited selective and potent dose- and time-dependent activity against the HeLa cell line. Results of the current study demonstrated notable <i>H</i>. <i>officinalis</i> medicinal potential, which calls for further investigation.https://www.mdpi.com/2223-7747/10/4/711<i>Hyssopus officinalis</i>antioxidant activityantigenotoxic activitycomet assaycytotoxic activityHeLa cell line |
| spellingShingle | Tijana Mićović Dijana Topalović Lada Živković Biljana Spremo-Potparević Vladimir Jakovljević Sanja Matić Suzana Popović Dejan Baskić Danijela Stešević Stevan Samardžić Danilo Stojanović Zoran Maksimović Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) Plants <i>Hyssopus officinalis</i> antioxidant activity antigenotoxic activity comet assay cytotoxic activity HeLa cell line |
| title | Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) |
| title_full | Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) |
| title_fullStr | Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) |
| title_full_unstemmed | Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) |
| title_short | Antioxidant, Antigenotoxic and Cytotoxic Activity of Essential Oils and Methanol Extracts of <i>Hyssopus officinalis</i> L. Subsp. <i>aristatus</i> (Godr.) Nyman (Lamiaceae) |
| title_sort | antioxidant antigenotoxic and cytotoxic activity of essential oils and methanol extracts of i hyssopus officinalis i l subsp i aristatus i godr nyman lamiaceae |
| topic | <i>Hyssopus officinalis</i> antioxidant activity antigenotoxic activity comet assay cytotoxic activity HeLa cell line |
| url | https://www.mdpi.com/2223-7747/10/4/711 |
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