Gene expression analysis of FABP4 in gastric cancer
Purpose: Gastric cancer has high incidence and mortality rate in several countries and is still one of the most frequent and lethal disease. In this study, we aimed to determine diagnostic markers in gastric cancer by molecular techniques; include mRNA expression analysis of FABP4 gene. Fatty acid b...
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| Format: | Article |
| Language: | English |
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Cukurova University
2016-06-01
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| Series: | Cukurova Medical Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/206100 |
| _version_ | 1797919179629658112 |
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| author | Abdulkarim Karim |
| author_facet | Abdulkarim Karim |
| author_sort | Abdulkarim Karim |
| collection | DOAJ |
| description | Purpose: Gastric cancer has high incidence and mortality rate in several countries and is still one of the most frequent and lethal disease. In this study, we aimed to determine diagnostic markers in gastric cancer by molecular techniques; include mRNA expression analysis of FABP4 gene. Fatty acid binding protein 4 (FABP4) gene encodes the fatty acid binding protein found in adipocytes. The protein encoded by FABP4 are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 47 gastric cancer. The mRNA expression level of FABP4 was measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). Results: The mRNA expression level of FABP4 was significantly decreased (down- regulated). Conclusion: Down-regulation of FABP4 gene seems to occur at the initial steps of gastric cancer development. In order to confirm the relationship between the gastric tumor and FABP4 gene, further analysis like immunohistochemistry and epigenetc techniques are necessary. |
| first_indexed | 2024-04-10T13:41:29Z |
| format | Article |
| id | doaj.art-a1bfe7c442bf44c7ad9a6aaf855d3302 |
| institution | Directory Open Access Journal |
| issn | 2602-3040 |
| language | English |
| last_indexed | 2024-04-10T13:41:29Z |
| publishDate | 2016-06-01 |
| publisher | Cukurova University |
| record_format | Article |
| series | Cukurova Medical Journal |
| spelling | doaj.art-a1bfe7c442bf44c7ad9a6aaf855d33022023-02-15T16:11:09ZengCukurova UniversityCukurova Medical Journal2602-30402016-06-0141224825248Gene expression analysis of FABP4 in gastric cancerAbdulkarim KarimPurpose: Gastric cancer has high incidence and mortality rate in several countries and is still one of the most frequent and lethal disease. In this study, we aimed to determine diagnostic markers in gastric cancer by molecular techniques; include mRNA expression analysis of FABP4 gene. Fatty acid binding protein 4 (FABP4) gene encodes the fatty acid binding protein found in adipocytes. The protein encoded by FABP4 are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. Material and Methods: Total RNA were extracted from paired tumor and normal tissues of 47 gastric cancer. The mRNA expression level of FABP4 was measured employing semi- quantitative reverse transcription- polymerase chain reaction (RT- PCR). Results: The mRNA expression level of FABP4 was significantly decreased (down- regulated). Conclusion: Down-regulation of FABP4 gene seems to occur at the initial steps of gastric cancer development. In order to confirm the relationship between the gastric tumor and FABP4 gene, further analysis like immunohistochemistry and epigenetc techniques are necessary.https://dergipark.org.tr/tr/download/article-file/206100gastrik kanser fabp4 ekspresyon analizi yarı-kantitatif rt-pcr |
| spellingShingle | Abdulkarim Karim Gene expression analysis of FABP4 in gastric cancer Cukurova Medical Journal gastrik kanser fabp4 ekspresyon analizi yarı-kantitatif rt-pcr |
| title | Gene expression analysis of FABP4 in gastric cancer |
| title_full | Gene expression analysis of FABP4 in gastric cancer |
| title_fullStr | Gene expression analysis of FABP4 in gastric cancer |
| title_full_unstemmed | Gene expression analysis of FABP4 in gastric cancer |
| title_short | Gene expression analysis of FABP4 in gastric cancer |
| title_sort | gene expression analysis of fabp4 in gastric cancer |
| topic | gastrik kanser fabp4 ekspresyon analizi yarı-kantitatif rt-pcr |
| url | https://dergipark.org.tr/tr/download/article-file/206100 |
| work_keys_str_mv | AT abdulkarimkarim geneexpressionanalysisoffabp4ingastriccancer |