Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei
<p>Abstract</p> <p>Background</p> <p>The control of tuberculosis in densely populated cities is complicated by close human-to-human contacts and potential transmission of pathogens from multiple sources. We conducted a molecular epidemiologic analysis of 356 <it>M...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-12-01
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| Series: | BMC Infectious Diseases |
| Online Access: | http://www.biomedcentral.com/1471-2334/8/170 |
| _version_ | 1828213151882018816 |
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| author | Su Ih-Jen Lee Shi-Yi Tsai Wen-Shing Sun Jun-Ren Chang Jia-Ru Lin Chih-Wei Tseng Fan-Chen Dou Horng-Yunn Lu Jang-Jih |
| author_facet | Su Ih-Jen Lee Shi-Yi Tsai Wen-Shing Sun Jun-Ren Chang Jia-Ru Lin Chih-Wei Tseng Fan-Chen Dou Horng-Yunn Lu Jang-Jih |
| author_sort | Su Ih-Jen |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The control of tuberculosis in densely populated cities is complicated by close human-to-human contacts and potential transmission of pathogens from multiple sources. We conducted a molecular epidemiologic analysis of 356 <it>Mycobacterium tuberculosis </it>(MTB) isolates from patients presenting pulmonary tuberculosis in metropolitan Taipei. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping, were applied after culture.</p> <p>Methods</p> <p>A total of 356 isolates were genotyped by standard spoligotyping and the strains were compared with in the international spoligotyping database (SpolDB4). All isolates were also categorized using the 15 loci MIRU-VNTR typing method and combin with <it>NTF </it>locus and RD deletion analyses.</p> <p>Results</p> <p>Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not identified in the database. Major spoligotypes found were Beijing lineages (52.5%), followed by Haarlem lineages (13.5%) and EAI plus EAI-like lineages (11%). When MIRU-VNTR was employed, 140 patterns were identified, including 36 clusters by 252 isolates and 104 unique patterns, and the largest cluster comprised 95 isolates from the Beijing family. The combination of spoligotyping and MIRU-VNTR revealed that 236 (67%) of the 356 isolates were clustered in 43 genotypes. Strains of the Beijing family was more likely to be of modern strain and a higher percentage of multiple drug resistance than other families combined (P = 0.08). Patients infected with Beijing strains were younger than those with other strains (mean 58.7 vs. 64.2, p = 0.02). Moreover, 85.3% of infected persons younger than 25 years had Beijing modern strain, suggesting a possible recent spread in the young population by this family of TB strain in Taipei.</p> <p>Conclusion</p> <p>Our data on MTB genotype in Taipei suggest that MTB infection has not been optimally controlled. Control efforts should be reinforced in view of the high prevalence of the Beijing strain in young population and association with drug resistance.</p> |
| first_indexed | 2024-04-12T14:46:17Z |
| format | Article |
| id | doaj.art-a1c0d6dd173d402a9a93e5f596c48879 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-04-12T14:46:17Z |
| publishDate | 2008-12-01 |
| publisher | BMC |
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| series | BMC Infectious Diseases |
| spelling | doaj.art-a1c0d6dd173d402a9a93e5f596c488792022-12-22T03:28:38ZengBMCBMC Infectious Diseases1471-23342008-12-018117010.1186/1471-2334-8-170Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in TaipeiSu Ih-JenLee Shi-YiTsai Wen-ShingSun Jun-RenChang Jia-RuLin Chih-WeiTseng Fan-ChenDou Horng-YunnLu Jang-Jih<p>Abstract</p> <p>Background</p> <p>The control of tuberculosis in densely populated cities is complicated by close human-to-human contacts and potential transmission of pathogens from multiple sources. We conducted a molecular epidemiologic analysis of 356 <it>Mycobacterium tuberculosis </it>(MTB) isolates from patients presenting pulmonary tuberculosis in metropolitan Taipei. Classical antibiogram studies and genetic characterization, using mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) typing and spoligotyping, were applied after culture.</p> <p>Methods</p> <p>A total of 356 isolates were genotyped by standard spoligotyping and the strains were compared with in the international spoligotyping database (SpolDB4). All isolates were also categorized using the 15 loci MIRU-VNTR typing method and combin with <it>NTF </it>locus and RD deletion analyses.</p> <p>Results</p> <p>Of 356 isolates spoligotyped, 290 (81.4%) displayed known spoligotypes and 66 were not identified in the database. Major spoligotypes found were Beijing lineages (52.5%), followed by Haarlem lineages (13.5%) and EAI plus EAI-like lineages (11%). When MIRU-VNTR was employed, 140 patterns were identified, including 36 clusters by 252 isolates and 104 unique patterns, and the largest cluster comprised 95 isolates from the Beijing family. The combination of spoligotyping and MIRU-VNTR revealed that 236 (67%) of the 356 isolates were clustered in 43 genotypes. Strains of the Beijing family was more likely to be of modern strain and a higher percentage of multiple drug resistance than other families combined (P = 0.08). Patients infected with Beijing strains were younger than those with other strains (mean 58.7 vs. 64.2, p = 0.02). Moreover, 85.3% of infected persons younger than 25 years had Beijing modern strain, suggesting a possible recent spread in the young population by this family of TB strain in Taipei.</p> <p>Conclusion</p> <p>Our data on MTB genotype in Taipei suggest that MTB infection has not been optimally controlled. Control efforts should be reinforced in view of the high prevalence of the Beijing strain in young population and association with drug resistance.</p>http://www.biomedcentral.com/1471-2334/8/170 |
| spellingShingle | Su Ih-Jen Lee Shi-Yi Tsai Wen-Shing Sun Jun-Ren Chang Jia-Ru Lin Chih-Wei Tseng Fan-Chen Dou Horng-Yunn Lu Jang-Jih Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei BMC Infectious Diseases |
| title | Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei |
| title_full | Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei |
| title_fullStr | Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei |
| title_full_unstemmed | Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei |
| title_short | Molecular epidemiology and evolutionary genetics of <it>Mycobacterium tuberculosis </it>in Taipei |
| title_sort | molecular epidemiology and evolutionary genetics of it mycobacterium tuberculosis it in taipei |
| url | http://www.biomedcentral.com/1471-2334/8/170 |
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