Genetic editing of the virulence gene of Escherichia coli using the CRISPR system
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is an emerging gene-editing technology that is widely used in prokaryotes and eukaryotes. It can realize the specific manipulation of the genome efficiently and accurately. CRISPR/Cas9 coupled λ-Red recombination technology was...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2020-04-01
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| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/8881.pdf |