Genetic editing of the virulence gene of Escherichia coli using the CRISPR system

Genetic editing of the virulence gene of Escherichia coli using the CRISPR system

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is an emerging gene-editing technology that is widely used in prokaryotes and eukaryotes. It can realize the specific manipulation of the genome efficiently and accurately. CRISPR/Cas9 coupled λ-Red recombination technology was...

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Bibliographic Details
Main Authors: Meijia Hou, Simeng Sun, Qizheng Feng, Xiumei Dong, Ping Zhang, Bo Shi, Jiali Liu, Dongfang Shi
Format: Article
Language:English
Published: PeerJ Inc. 2020-04-01
Series:PeerJ
Subjects:
CRISPR-Cas9
Virulence genes
ETEC
Single base mutation
CDS
Online Access:https://peerj.com/articles/8881.pdf

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