An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker
We report an alpha – fetoprotein (AFP) cancer biomarker electrochemical immunosensor detected on a carbon nanofiber (CNF) and gold nanoparticles (AuNPs) platform. Functionalised carbon nanofiber and gold nanoparticles were immobilised through drop drying and electrodeposition techniques respectively...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Sensing and Bio-Sensing Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180423000260 |
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| author | Foluke O.G. Olorundare Dimpo S. Sipuka Tsholofelo I. Sebokolodi Sesethu Makaluza Nyasha Midzi Tetsuya Kodama Omotayo A. Arotiba Duduzile Nkosi |
| author_facet | Foluke O.G. Olorundare Dimpo S. Sipuka Tsholofelo I. Sebokolodi Sesethu Makaluza Nyasha Midzi Tetsuya Kodama Omotayo A. Arotiba Duduzile Nkosi |
| author_sort | Foluke O.G. Olorundare |
| collection | DOAJ |
| description | We report an alpha – fetoprotein (AFP) cancer biomarker electrochemical immunosensor detected on a carbon nanofiber (CNF) and gold nanoparticles (AuNPs) platform. Functionalised carbon nanofiber and gold nanoparticles were immobilised through drop drying and electrodeposition techniques respectively on a glassy carbon electrode (GCE). AFP antibody was immobilised on the platform overnight followed by blocking of nonspecific binding sites with bovine serum albumin (BSA) for 4 h. The AFP antigen was incubated on the platform for 40 min at 35 °C for the antigen – antibody interaction. CNF and AuNPs were characterised by electron microscopy and Fourier transform infrared (FTIR) spectroscopy. The modified electrodes and the immunosensor were characterised by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The electrochemical response of the GCE/CNF/AuNPs electrode and the resulting immunosensor was significantly enhanced by the nanocomposite of CNF and AuNPs. The electrochemical immunosensing measurement for AFP was carried out under optimal conditions at a concentration range from 0.005 to 500 ng mL−1 in 5 mM ferri/ferrocyanide solution. A low limit of detection of 0.50 pg mL−1 from square wave voltammetry (SWV) and 0.48 pg mL−1 from EIS measurements were obtained. The immunosensor exhibited good sensitivity, good selectivity, good repeatability, and was stable at 4 °C over a period of three weeks. |
| first_indexed | 2024-03-12T13:10:28Z |
| format | Article |
| id | doaj.art-a1c830f8cacb43bcb1c3a6af80add1d7 |
| institution | Directory Open Access Journal |
| issn | 2214-1804 |
| language | English |
| last_indexed | 2024-03-12T13:10:28Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj.art-a1c830f8cacb43bcb1c3a6af80add1d72023-08-28T04:22:48ZengElsevierSensing and Bio-Sensing Research2214-18042023-08-0141100574An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarkerFoluke O.G. Olorundare0Dimpo S. Sipuka1Tsholofelo I. Sebokolodi2Sesethu Makaluza3Nyasha Midzi4Tetsuya Kodama5Omotayo A. Arotiba6Duduzile Nkosi7Department of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South AfricaDepartment of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South AfricaLaboratory of Biomedical Engineering for Cancer, Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai, Miyagi 980-8575, JapanDepartment of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, South Africa; Corresponding author at: Department of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South Africa.Department of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South Africa; Centre for Nanomaterials Science Research, University of Johannesburg, South Africa; Corresponding author at: Department of Chemical Sciences, University of Johannesburg, Doornfontein 2028, Johannesburg, South Africa.We report an alpha – fetoprotein (AFP) cancer biomarker electrochemical immunosensor detected on a carbon nanofiber (CNF) and gold nanoparticles (AuNPs) platform. Functionalised carbon nanofiber and gold nanoparticles were immobilised through drop drying and electrodeposition techniques respectively on a glassy carbon electrode (GCE). AFP antibody was immobilised on the platform overnight followed by blocking of nonspecific binding sites with bovine serum albumin (BSA) for 4 h. The AFP antigen was incubated on the platform for 40 min at 35 °C for the antigen – antibody interaction. CNF and AuNPs were characterised by electron microscopy and Fourier transform infrared (FTIR) spectroscopy. The modified electrodes and the immunosensor were characterised by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. The electrochemical response of the GCE/CNF/AuNPs electrode and the resulting immunosensor was significantly enhanced by the nanocomposite of CNF and AuNPs. The electrochemical immunosensing measurement for AFP was carried out under optimal conditions at a concentration range from 0.005 to 500 ng mL−1 in 5 mM ferri/ferrocyanide solution. A low limit of detection of 0.50 pg mL−1 from square wave voltammetry (SWV) and 0.48 pg mL−1 from EIS measurements were obtained. The immunosensor exhibited good sensitivity, good selectivity, good repeatability, and was stable at 4 °C over a period of three weeks.http://www.sciencedirect.com/science/article/pii/S2214180423000260Alpha-fetoprotein (AFP)Carbon nanofiberGold nanoparticlesElectrochemical immunosensorcancer biomarker |
| spellingShingle | Foluke O.G. Olorundare Dimpo S. Sipuka Tsholofelo I. Sebokolodi Sesethu Makaluza Nyasha Midzi Tetsuya Kodama Omotayo A. Arotiba Duduzile Nkosi An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker Sensing and Bio-Sensing Research Alpha-fetoprotein (AFP) Carbon nanofiber Gold nanoparticles Electrochemical immunosensor cancer biomarker |
| title | An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker |
| title_full | An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker |
| title_fullStr | An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker |
| title_full_unstemmed | An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker |
| title_short | An electrochemical immunosensor on a carbon nanofiber/gold nanoparticles platform for the detection of alpha-feto protein cancer biomarker |
| title_sort | electrochemical immunosensor on a carbon nanofiber gold nanoparticles platform for the detection of alpha feto protein cancer biomarker |
| topic | Alpha-fetoprotein (AFP) Carbon nanofiber Gold nanoparticles Electrochemical immunosensor cancer biomarker |
| url | http://www.sciencedirect.com/science/article/pii/S2214180423000260 |
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