Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects
Introduction: The chemical fungicides are used widely in the world. To reduce the application of synthetic fungicides in treating plant diseases, biological methods are considered as an alternative way to control plant diseases. Many actinomycetes, particularly Streptomyces species are biological ag...
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| Format: | Article |
| Language: | English |
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University of Isfahan
2015-12-01
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| Series: | Biological Journal of Microorganism |
| Subjects: | |
| Online Access: | http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-592-2&slc_lang=en&sid=1 |
| _version_ | 1811213905491394560 |
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| author | Alireza Dehnad Ebrahim Esmaili Mahmood Solouki |
| author_facet | Alireza Dehnad Ebrahim Esmaili Mahmood Solouki |
| author_sort | Alireza Dehnad |
| collection | DOAJ |
| description | Introduction: The chemical fungicides are used widely in the world. To reduce the application of synthetic fungicides in treating plant diseases, biological methods are considered as an alternative way to control plant diseases. Many actinomycetes, particularly Streptomyces species are biological agents against a broad spectrum of fungal plant pathogens. The purpose of this study was using the kitinolitik actinomycetes isolated from soil of Eastern Azerbaijan province In order to produce biological pesticides.
Materials and methods: Soil samples were taken from different areas of Eastern Azerbaijan province. According to Streptomyces morphological features, single colonies were isolated. To identify the bacteria by molecular characteristic, the genomic DNA was extracted and then the sequences of 16S rDNA were replicated. By using specific primers the bacterial isolates containing chitinase gene were screened. The isolates consisted Chitinase enzyme and were antagonistically cultured with Alternaria genus which is a fungal plant pathogen.
Results: Out of 60 soil collected samples, 31 Streptomyces bacterial isolates were separated. Four isolates showed positive results to selectivity action of the chitinase enzyme. Treatment of 3 bacterial isolates with 2 pathogenic fungi showed that AE09 is the most effective anti-fungal isolates.
Discussion and conclusion: Soils in Eastern Azerbaijan province are rich of Streptomyces bacteria which generate antifungal compounds. Obtaining the Streptomyces bacteria which have chitinase gene, can lead to identification of very effective strains as anti-fungal. |
| first_indexed | 2024-04-12T05:55:13Z |
| format | Article |
| id | doaj.art-a1c9b85c4101460293ef9b4f48539021 |
| institution | Directory Open Access Journal |
| issn | 2322-5173 2322-5181 |
| language | English |
| last_indexed | 2024-04-12T05:55:13Z |
| publishDate | 2015-12-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | Biological Journal of Microorganism |
| spelling | doaj.art-a1c9b85c4101460293ef9b4f485390212022-12-22T03:45:11ZengUniversity of IsfahanBiological Journal of Microorganism2322-51732322-51812015-12-01415123134Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effectsAlireza Dehnad0Ebrahim Esmaili1Mahmood Solouki2 Assistant Professor of Microbial biotechnology, East Azerbaijan Agricultural Education Center, AREEO, Tabriz, Iran, dehnadar@yahoo.com & Adehnad@abrii.ac.ir M.Sc. of Biotechnology, Zabol University, Iran, ab.esmaili60@gmail.com Associate Professor of Plant Biotechnology, Zabol University, Biocenter, Iran Introduction: The chemical fungicides are used widely in the world. To reduce the application of synthetic fungicides in treating plant diseases, biological methods are considered as an alternative way to control plant diseases. Many actinomycetes, particularly Streptomyces species are biological agents against a broad spectrum of fungal plant pathogens. The purpose of this study was using the kitinolitik actinomycetes isolated from soil of Eastern Azerbaijan province In order to produce biological pesticides. Materials and methods: Soil samples were taken from different areas of Eastern Azerbaijan province. According to Streptomyces morphological features, single colonies were isolated. To identify the bacteria by molecular characteristic, the genomic DNA was extracted and then the sequences of 16S rDNA were replicated. By using specific primers the bacterial isolates containing chitinase gene were screened. The isolates consisted Chitinase enzyme and were antagonistically cultured with Alternaria genus which is a fungal plant pathogen. Results: Out of 60 soil collected samples, 31 Streptomyces bacterial isolates were separated. Four isolates showed positive results to selectivity action of the chitinase enzyme. Treatment of 3 bacterial isolates with 2 pathogenic fungi showed that AE09 is the most effective anti-fungal isolates. Discussion and conclusion: Soils in Eastern Azerbaijan province are rich of Streptomyces bacteria which generate antifungal compounds. Obtaining the Streptomyces bacteria which have chitinase gene, can lead to identification of very effective strains as anti-fungal.http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-592-2&slc_lang=en&sid=1Streptomyces Chitinase gene 16S rDNA Biological control |
| spellingShingle | Alireza Dehnad Ebrahim Esmaili Mahmood Solouki Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects Biological Journal of Microorganism Streptomyces Chitinase gene 16S rDNA Biological control |
| title | Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects |
| title_full | Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects |
| title_fullStr | Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects |
| title_full_unstemmed | Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects |
| title_short | Isolation and molecular identification chitinase-producing Streptomyces strains and examination of their in-vitro antagonistic effects |
| title_sort | isolation and molecular identification chitinase producing streptomyces strains and examination of their in vitro antagonistic effects |
| topic | Streptomyces Chitinase gene 16S rDNA Biological control |
| url | http://uijs.ui.ac.ir/bjm/browse.php?a_code=A-10-592-2&slc_lang=en&sid=1 |
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