Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain

Protocol for viral vector-mediated measurement of transcription factor activity of mouse brain

Summary: Here, we provide a step-by-step protocol to measure the activities of multiple transcription factors (TFs) in the same mouse brain. This protocol includes a procedure to construct a virus-based TF activity reporter, in utero transfection, and PCR-based measurement of TF activity to obtain t...

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Bibliographic Details
Main Authors:	Hajime Yamamoto, Kentaro Abe
Format:	Article
Language:	English
Published:	Elsevier 2022-09-01
Series:	STAR Protocols
Subjects:	Cell Biology Developmental biology Molecular Biology Gene Expression Neuroscience
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166722005135

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