Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives
Electrophoretical and histological analysis were performed on Mytilus galloprovincialis digestive gland samples, in order to detect the presence of a previously identified protein ca 30 kDa MW, synthesized during Dinophysis spp. blooms, and assess a possible correlation between the occurrence of thi...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Modena and Reggio Emilia
2004-11-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | https://isj02.unimore.it/index.php/ISJ/article/view/95 |
| _version_ | 1818865850583613440 |
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| author | R Auriemma S Battistella |
| author_facet | R Auriemma S Battistella |
| author_sort | R Auriemma |
| collection | DOAJ |
| description | Electrophoretical and histological analysis were performed on Mytilus galloprovincialis digestive gland samples, in order to detect the presence of a previously identified protein ca 30 kDa MW, synthesized during Dinophysis spp. blooms, and assess a possible correlation between the occurrence of this protein and okadaic acid (OA) exposure by ingestion. Mussels were sampled monthly from July 2000 to November 2001 in the Gulf of Trieste (upper Adriatic Sea) and immediately processed. Parallel samples were maintained in sea water plastic tanks and fed with marine invertebrate feed mixed with OA and derivatives at different concentration of toxins for each experimental group (25 mg, 50 mg, 100 mg). In tank reared mussels fed with OA, degeneration of digestive cells and appearance of 24.6 kDa protein were observed, while in wild mussels, neither histological alterations nor presence of a 24.6 kDa protein, were detected. A correlation between the toxins concentration and time of appearance was highlighted, to demonstrate this protein is synthesized in response to OA and derivatives
exposure. About the identity of 24.6 kDa protein, it could be an enzyme involved in detoxification reactions, probably Glyoxalase I. |
| first_indexed | 2024-12-19T10:54:06Z |
| format | Article |
| id | doaj.art-a1d3342dd2db4b8cae76ca542bdf48be |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-19T10:54:06Z |
| publishDate | 2004-11-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-a1d3342dd2db4b8cae76ca542bdf48be2022-12-21T20:24:52ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2004-11-0111Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivativesR Auriemma0S Battistella1Department of Biology, University of Trieste, Trieste, ItalyDepartment of Biology, University of Trieste, Trieste, ItalyElectrophoretical and histological analysis were performed on Mytilus galloprovincialis digestive gland samples, in order to detect the presence of a previously identified protein ca 30 kDa MW, synthesized during Dinophysis spp. blooms, and assess a possible correlation between the occurrence of this protein and okadaic acid (OA) exposure by ingestion. Mussels were sampled monthly from July 2000 to November 2001 in the Gulf of Trieste (upper Adriatic Sea) and immediately processed. Parallel samples were maintained in sea water plastic tanks and fed with marine invertebrate feed mixed with OA and derivatives at different concentration of toxins for each experimental group (25 mg, 50 mg, 100 mg). In tank reared mussels fed with OA, degeneration of digestive cells and appearance of 24.6 kDa protein were observed, while in wild mussels, neither histological alterations nor presence of a 24.6 kDa protein, were detected. A correlation between the toxins concentration and time of appearance was highlighted, to demonstrate this protein is synthesized in response to OA and derivatives exposure. About the identity of 24.6 kDa protein, it could be an enzyme involved in detoxification reactions, probably Glyoxalase I.https://isj02.unimore.it/index.php/ISJ/article/view/95musselsdigestive glandokadaic acidDSPMytilus galloprovincialis |
| spellingShingle | R Auriemma S Battistella Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives Invertebrate Survival Journal mussels digestive gland okadaic acid DSP Mytilus galloprovincialis |
| title | Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| title_full | Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| title_fullStr | Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| title_full_unstemmed | Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| title_short | Biochemical and histological alterations of Mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| title_sort | biochemical and histological alterations of mytilus galloprovincialis digestive gland after exposure to okadaic acid and derivatives |
| topic | mussels digestive gland okadaic acid DSP Mytilus galloprovincialis |
| url | https://isj02.unimore.it/index.php/ISJ/article/view/95 |
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