Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events
The proper regulation of cell cycle transitions is paramount to the maintenance of cellular genome integrity. In Saccharomyces cerevisiae, the mitotic exit network (MEN) is a Ras-like signaling cascade that effects the transition from M phase to G1 during the cell division cycle in budding yeast. ME...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Oxford University Press
2018-11-01
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| Series: | G3: Genes, Genomes, Genetics |
| Subjects: | |
| Online Access: | http://g3journal.org/lookup/doi/10.1534/g3.118.200672 |
| _version_ | 1818865011354763264 |
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| author | Jenna Whalen Courtney Sniffen Siobhan Gartland Michael Vannini Anupama Seshan |
| author_facet | Jenna Whalen Courtney Sniffen Siobhan Gartland Michael Vannini Anupama Seshan |
| author_sort | Jenna Whalen |
| collection | DOAJ |
| description | The proper regulation of cell cycle transitions is paramount to the maintenance of cellular genome integrity. In Saccharomyces cerevisiae, the mitotic exit network (MEN) is a Ras-like signaling cascade that effects the transition from M phase to G1 during the cell division cycle in budding yeast. MEN activation is tightly regulated. It occurs during anaphase and is coupled to mitotic spindle position by the spindle position checkpoint (SPoC). Bfa1 is a key component of the SPoC and functions as part of a two-component GAP complex along with Bub2. The GAP activity of Bfa1-Bub2 keeps the MEN GTPase Tem1 inactive in cells with mispositioned spindles, thereby preventing inappropriate mitotic exit and preserving genome integrity. Interestingly, a GAP-independent role for Bfa1 in mitotic exit regulation has been previously identified. However the nature of this Bub2-independent role and its biological significance are not understood. Here we show that Bfa1 also activates the MEN by promoting the localization of Tem1 primarily to the daughter spindle pole body (dSPB). We demonstrate that the overexpression of BFA1 is lethal due to defects in Tem1 localization, which is required for its activity. In addition, our studies demonstrate a Tem1-independent role for Bfa1 in promoting proper cytokinesis. Cells lacking TEM1, in which the essential mitotic exit function is bypassed, exhibit cytokinesis defects. These defects are suppressed by the overexpression of BFA1. We conclude that Bfa1 functions to both inhibit and activate late mitotic events. |
| first_indexed | 2024-12-19T10:40:46Z |
| format | Article |
| id | doaj.art-a1d33738ddb6436c92719cacc135d944 |
| institution | Directory Open Access Journal |
| issn | 2160-1836 |
| language | English |
| last_indexed | 2024-12-19T10:40:46Z |
| publishDate | 2018-11-01 |
| publisher | Oxford University Press |
| record_format | Article |
| series | G3: Genes, Genomes, Genetics |
| spelling | doaj.art-a1d33738ddb6436c92719cacc135d9442022-12-21T20:25:28ZengOxford University PressG3: Genes, Genomes, Genetics2160-18362018-11-018113397341010.1534/g3.118.2006722Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic EventsJenna WhalenCourtney SniffenSiobhan GartlandMichael VanniniAnupama SeshanThe proper regulation of cell cycle transitions is paramount to the maintenance of cellular genome integrity. In Saccharomyces cerevisiae, the mitotic exit network (MEN) is a Ras-like signaling cascade that effects the transition from M phase to G1 during the cell division cycle in budding yeast. MEN activation is tightly regulated. It occurs during anaphase and is coupled to mitotic spindle position by the spindle position checkpoint (SPoC). Bfa1 is a key component of the SPoC and functions as part of a two-component GAP complex along with Bub2. The GAP activity of Bfa1-Bub2 keeps the MEN GTPase Tem1 inactive in cells with mispositioned spindles, thereby preventing inappropriate mitotic exit and preserving genome integrity. Interestingly, a GAP-independent role for Bfa1 in mitotic exit regulation has been previously identified. However the nature of this Bub2-independent role and its biological significance are not understood. Here we show that Bfa1 also activates the MEN by promoting the localization of Tem1 primarily to the daughter spindle pole body (dSPB). We demonstrate that the overexpression of BFA1 is lethal due to defects in Tem1 localization, which is required for its activity. In addition, our studies demonstrate a Tem1-independent role for Bfa1 in promoting proper cytokinesis. Cells lacking TEM1, in which the essential mitotic exit function is bypassed, exhibit cytokinesis defects. These defects are suppressed by the overexpression of BFA1. We conclude that Bfa1 functions to both inhibit and activate late mitotic events.http://g3journal.org/lookup/doi/10.1534/g3.118.200672Bfa1Tem1MENmitotic exitcytokinesis |
| spellingShingle | Jenna Whalen Courtney Sniffen Siobhan Gartland Michael Vannini Anupama Seshan Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events G3: Genes, Genomes, Genetics Bfa1 Tem1 MEN mitotic exit cytokinesis |
| title | Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events |
| title_full | Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events |
| title_fullStr | Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events |
| title_full_unstemmed | Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events |
| title_short | Budding Yeast BFA1 Has Multiple Positive Roles in Directing Late Mitotic Events |
| title_sort | budding yeast bfa1 has multiple positive roles in directing late mitotic events |
| topic | Bfa1 Tem1 MEN mitotic exit cytokinesis |
| url | http://g3journal.org/lookup/doi/10.1534/g3.118.200672 |
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