Phage p1-derived artificial chromosomes facilitate heterologous expression of the FK506 gene cluster.

Phage p1-derived artificial chromosomes facilitate heterologous expression of the FK506 gene cluster.

We describe a procedure for the conjugative transfer of phage P1-derived Artificial Chromosome (PAC) library clones containing large natural product gene clusters (≥70 kilobases) to Streptomyces coelicolor strains that have been engineered for improved heterologous production of natural products. Th...

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Bibliographic Details
Main Authors: Adam C Jones, Bertolt Gust, Andreas Kulik, Lutz Heide, Mark J Buttner, Mervyn J Bibb
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3708917?pdf=render

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