ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas
Bivalves are filter-feeding organisms and biomarkers of bacterial pollution. Our study aimed to analyze the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing <i>Escherichia coli</i> among bivalves. A total of 522 bivalve samples were coll...
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MDPI AG
2023-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/2/415 |
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| author | Samanta Freire Teresa Grilo Bruna Rodrigues Rui Oliveira Carla Esteves António Marques Laurent Poirel Marta Aires-de-Sousa |
| author_facet | Samanta Freire Teresa Grilo Bruna Rodrigues Rui Oliveira Carla Esteves António Marques Laurent Poirel Marta Aires-de-Sousa |
| author_sort | Samanta Freire |
| collection | DOAJ |
| description | Bivalves are filter-feeding organisms and biomarkers of bacterial pollution. Our study aimed to analyze the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing <i>Escherichia coli</i> among bivalves. A total of 522 bivalve samples were collected along Portuguese shellfish production areas. Homogenized samples were screened for <i>E. coli</i> contamination on corresponding selective plates, allowing for concomitant growth of <i>Klebsiella pneumoniae</i>. <i>E. coli</i> growth was observed in 39% of the samples. Subsequent selective screening identified nine samples (4.4%) contaminated with ESBL producers, corresponding to <i>E. coli</i> (<i>n</i> = 7) and <i>K. pneumoniae</i> (<i>n</i> = 2), while a single carbapenemase-producing <i>K. pneumoniae</i> (0.5%) was identified. ESBLs were all CTX-M-types commonly identified in human isolates, i.e., CTX-M-32 (<i>n</i> = 4), CTX-M-15 (<i>n</i> = 4), and CTX-M-14 (<i>n</i> = 1). The carbapenemase producer harbored the <i>bla</i><sub>GES-5</sub> gene located on a ColE plasmid. Clonality was evaluated by multilocus sequence typing, identifying <i>E. coli</i> backgrounds as ST10, ST23, ST540, ST617, ST746, SLV206, and SLV2325, commonly identified among environmental and human strains. The <i>K. pneumoniae</i> isolates belonged to ST834, ST15, and DLV644. The occurrence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves reveals how the marine environment constitutes a reservoir of critical bacterial pathogens, thus potentially representing a risk to human health. |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T08:23:20Z |
| publishDate | 2023-02-01 |
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| spelling | doaj.art-a1d8b762418c4e05b725b2d2d74583fb2023-11-16T22:15:32ZengMDPI AGMicroorganisms2076-26072023-02-0111241510.3390/microorganisms11020415ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production AreasSamanta Freire0Teresa Grilo1Bruna Rodrigues2Rui Oliveira3Carla Esteves4António Marques5Laurent Poirel6Marta Aires-de-Sousa7Laboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalLaboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalLaboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalInstituto Português do Mar e Atmosfera, 1495-165 Lisboa, PortugalInstituto Português do Mar e Atmosfera, 1495-165 Lisboa, PortugalInstituto Português do Mar e Atmosfera, 1495-165 Lisboa, PortugalMedical and Molecular Microbiology Unit, Faculty of Science and Medicine, University of Fribourg, 1700 Fribourg, SwitzerlandLaboratory of Molecular Biology, Portuguese Red Cross, 1600-680 Lisboa, PortugalBivalves are filter-feeding organisms and biomarkers of bacterial pollution. Our study aimed to analyze the occurrence and characteristics of extended-spectrum β-lactamase (ESBL)- and carbapenemase-producing <i>Escherichia coli</i> among bivalves. A total of 522 bivalve samples were collected along Portuguese shellfish production areas. Homogenized samples were screened for <i>E. coli</i> contamination on corresponding selective plates, allowing for concomitant growth of <i>Klebsiella pneumoniae</i>. <i>E. coli</i> growth was observed in 39% of the samples. Subsequent selective screening identified nine samples (4.4%) contaminated with ESBL producers, corresponding to <i>E. coli</i> (<i>n</i> = 7) and <i>K. pneumoniae</i> (<i>n</i> = 2), while a single carbapenemase-producing <i>K. pneumoniae</i> (0.5%) was identified. ESBLs were all CTX-M-types commonly identified in human isolates, i.e., CTX-M-32 (<i>n</i> = 4), CTX-M-15 (<i>n</i> = 4), and CTX-M-14 (<i>n</i> = 1). The carbapenemase producer harbored the <i>bla</i><sub>GES-5</sub> gene located on a ColE plasmid. Clonality was evaluated by multilocus sequence typing, identifying <i>E. coli</i> backgrounds as ST10, ST23, ST540, ST617, ST746, SLV206, and SLV2325, commonly identified among environmental and human strains. The <i>K. pneumoniae</i> isolates belonged to ST834, ST15, and DLV644. The occurrence of ESBL- and carbapenemase-producing Enterobacteriaceae in bivalves reveals how the marine environment constitutes a reservoir of critical bacterial pathogens, thus potentially representing a risk to human health.https://www.mdpi.com/2076-2607/11/2/415ESBLcarbapenemases<i>Escherichia coli</i><i>Klebsiella pneumoniae</i>bivalvesPortugal |
| spellingShingle | Samanta Freire Teresa Grilo Bruna Rodrigues Rui Oliveira Carla Esteves António Marques Laurent Poirel Marta Aires-de-Sousa ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas Microorganisms ESBL carbapenemases <i>Escherichia coli</i> <i>Klebsiella pneumoniae</i> bivalves Portugal |
| title | ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas |
| title_full | ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas |
| title_fullStr | ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas |
| title_full_unstemmed | ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas |
| title_short | ESBL- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> among Bivalves from Portuguese Shellfish Production Areas |
| title_sort | esbl and carbapenemase producing i escherichia coli i and i klebsiella pneumoniae i among bivalves from portuguese shellfish production areas |
| topic | ESBL carbapenemases <i>Escherichia coli</i> <i>Klebsiella pneumoniae</i> bivalves Portugal |
| url | https://www.mdpi.com/2076-2607/11/2/415 |
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