Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery
The optodynamic process of laser-induced microbubble (MB) cavitation in liquids is utilized in various medical applications. However, how incident laser radiation interacts with MBs as an ultrasound contrast agent is rarely estimated when the liquid already contains stable MBs. The present study inv...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-10-01
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| Series: | Pharmaceutics |
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| Online Access: | http://www.mdpi.com/1999-4923/10/4/175 |
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| author | Ai-Ho Liao Ho-Chiao Chuang Bo-Ya Chang Wen-Chuan Kuo Chih-Hung Wang Hong-Wei Gao Chien-Ping Chiang |
| author_facet | Ai-Ho Liao Ho-Chiao Chuang Bo-Ya Chang Wen-Chuan Kuo Chih-Hung Wang Hong-Wei Gao Chien-Ping Chiang |
| author_sort | Ai-Ho Liao |
| collection | DOAJ |
| description | The optodynamic process of laser-induced microbubble (MB) cavitation in liquids is utilized in various medical applications. However, how incident laser radiation interacts with MBs as an ultrasound contrast agent is rarely estimated when the liquid already contains stable MBs. The present study investigated the efficacy of the laser-mediated cavitation of albumin-shelled MBs in enhancing transdermal drug delivery. Different types and conditions of laser-mediated inertial cavitation of MBs were first evaluated. A CO2 fractional pulsed laser was selected for combining with MBs in the in vitro and in vivo experiments. The in vitro skin penetration by β-arbutin after 2 h was 2 times greater in the group combining a laser with MBs than in the control group. In small-animal experiments, the whitening effect on the skin of C57BL/6J mice in the group combining a laser with MBs on the skin plus penetrating β-arbutin increased (significantly) by 48.0% at day 11 and 50.0% at day 14, and then tended to stabilize for the remainder of the 20-day experimental period. The present results indicate that combining a CO2 laser with albumin-shelled MBs can increase skin permeability so as to enhance the delivery of β-arbutin to inhibit melanogenesis in mice without damaging the skin. |
| first_indexed | 2024-04-11T18:05:30Z |
| format | Article |
| id | doaj.art-a1d914f5aa664d0fbcc058f4431e916d |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-04-11T18:05:30Z |
| publishDate | 2018-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-a1d914f5aa664d0fbcc058f4431e916d2022-12-22T04:10:20ZengMDPI AGPharmaceutics1999-49232018-10-0110417510.3390/pharmaceutics10040175pharmaceutics10040175Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug DeliveryAi-Ho Liao0Ho-Chiao Chuang1Bo-Ya Chang2Wen-Chuan Kuo3Chih-Hung Wang4Hong-Wei Gao5Chien-Ping Chiang6Graduate Institute of Biomedical Engineering, National Taiwan University of Science and Technology, Taipei 10607, TaiwanDepartment of Mechanical Engineering, National Taipei University of Technology, Taipei 10608, TaiwanDepartment of Mechanical Engineering, National Taipei University of Technology, Taipei 10608, TaiwanInstitute of Biophotonics, National Yang-Ming University, Taipei 11221, TaiwanGraduate Institute of Medical Sciences, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei 11490, TaiwanDepartment of Dermatology, Tri-Service General Hospital, National Defense Medical Center, No. 325, Sec. 2, Chenggong Rd., Neihu District, Taipei 11490, TaiwanThe optodynamic process of laser-induced microbubble (MB) cavitation in liquids is utilized in various medical applications. However, how incident laser radiation interacts with MBs as an ultrasound contrast agent is rarely estimated when the liquid already contains stable MBs. The present study investigated the efficacy of the laser-mediated cavitation of albumin-shelled MBs in enhancing transdermal drug delivery. Different types and conditions of laser-mediated inertial cavitation of MBs were first evaluated. A CO2 fractional pulsed laser was selected for combining with MBs in the in vitro and in vivo experiments. The in vitro skin penetration by β-arbutin after 2 h was 2 times greater in the group combining a laser with MBs than in the control group. In small-animal experiments, the whitening effect on the skin of C57BL/6J mice in the group combining a laser with MBs on the skin plus penetrating β-arbutin increased (significantly) by 48.0% at day 11 and 50.0% at day 14, and then tended to stabilize for the remainder of the 20-day experimental period. The present results indicate that combining a CO2 laser with albumin-shelled MBs can increase skin permeability so as to enhance the delivery of β-arbutin to inhibit melanogenesis in mice without damaging the skin.http://www.mdpi.com/1999-4923/10/4/175ultrasound contrast agentslasertransdermalcavitationarbutin |
| spellingShingle | Ai-Ho Liao Ho-Chiao Chuang Bo-Ya Chang Wen-Chuan Kuo Chih-Hung Wang Hong-Wei Gao Chien-Ping Chiang Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery Pharmaceutics ultrasound contrast agents laser transdermal cavitation arbutin |
| title | Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery |
| title_full | Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery |
| title_fullStr | Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery |
| title_full_unstemmed | Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery |
| title_short | Combining Microbubble Contrast Agent with Pulsed-Laser Irradiation for Transdermal Drug Delivery |
| title_sort | combining microbubble contrast agent with pulsed laser irradiation for transdermal drug delivery |
| topic | ultrasound contrast agents laser transdermal cavitation arbutin |
| url | http://www.mdpi.com/1999-4923/10/4/175 |
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