Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension
Background: In the development of hypertensive microvascular remodeling, a relevant role may be played by changes in extracellular matrix proteins. Aim of this study was the to evaluate some extracellular matrix components within the tunica media of subcutaneous small arteries in 9 normotensive subj...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-07-01
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| Series: | Blood Pressure |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/08037051.2018.1448256 |
| _version_ | 1797685094546145280 |
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| author | Gaia Favero Anna Paini Carolina De Ciuceis Luigi F. Rodella Enrico Moretti Enzo Porteri Claudia Rossini Silvia Ministrini Leonardo Solaini Caletti Stefano Maria Antonietta Coschignano Valeria Brami Alina Petelca Matteo Nardin Ilenia Valli Guido A. M. Tiberio Francesca Bonomini Claudia Agabiti Rosei Nazario Portolani Damiano Rizzoni Rita Rezzani |
| author_facet | Gaia Favero Anna Paini Carolina De Ciuceis Luigi F. Rodella Enrico Moretti Enzo Porteri Claudia Rossini Silvia Ministrini Leonardo Solaini Caletti Stefano Maria Antonietta Coschignano Valeria Brami Alina Petelca Matteo Nardin Ilenia Valli Guido A. M. Tiberio Francesca Bonomini Claudia Agabiti Rosei Nazario Portolani Damiano Rizzoni Rita Rezzani |
| author_sort | Gaia Favero |
| collection | DOAJ |
| description | Background: In the development of hypertensive microvascular remodeling, a relevant role may be played by changes in extracellular matrix proteins. Aim of this study was the to evaluate some extracellular matrix components within the tunica media of subcutaneous small arteries in 9 normotensive subjects and 12 essential hypertensive patients, submitted to a biopsy of subcutaneous fat from the gluteal or the anterior abdominal region. Patients and Methods: Subcutaneous small resistance arteries were dissected and mounted on an isometric myograph, and the tunica media to internal lumen ratio was measured. In addition, fibronectin, laminin, transforming growth factor-beta-1 (TGF-β1) and emilin-1 contents within the tunica media were evaluated by immunofluorescence and relative immunomorphometrical analysis (immunopositivity % of area). The total collagen content and collagen subtypes within the tunica media were evaluated using both Sirius red staining (under polarized light) and immunofluorescence assay. Results: Normotensive controls had less total and type III collagen in respect with hypertensive patients. Fibronectin and TGF-β1 tunica media content was significantly greater in essential hypertensive patients, compared with normotensive controls, while laminin and emilin-1 tunica media content was lesser in essential hypertensive patients, compared with normotensive controls. A significant correlation was observed between fibronectin tunica media content and media to lumen ratio. Conclusions: Our results indicate that, in small resistance arteries of patients with essential hypertension, a relevant fibrosis may be detected; fibronectin and TGF-β1 tunica media content is increased, while laminin and emilin-1 content is decreased; these changes might be involved in the development of small resistance artery remodeling in humans. |
| first_indexed | 2024-03-12T00:39:21Z |
| format | Article |
| id | doaj.art-a1da0857a4d6490cb7f650efa5a42263 |
| institution | Directory Open Access Journal |
| issn | 0803-7051 1651-1999 |
| language | English |
| last_indexed | 2024-03-12T00:39:21Z |
| publishDate | 2018-07-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Blood Pressure |
| spelling | doaj.art-a1da0857a4d6490cb7f650efa5a422632023-09-15T08:45:22ZengTaylor & Francis GroupBlood Pressure0803-70511651-19992018-07-0127423123910.1080/08037051.2018.14482561448256Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertensionGaia Favero0Anna Paini1Carolina De Ciuceis2Luigi F. Rodella3Enrico Moretti4Enzo Porteri5Claudia Rossini6Silvia Ministrini7Leonardo Solaini8Caletti Stefano9Maria Antonietta Coschignano10Valeria Brami11Alina Petelca12Matteo Nardin13Ilenia Valli14Guido A. M. Tiberio15Francesca Bonomini16Claudia Agabiti Rosei17Nazario Portolani18Damiano Rizzoni19Rita Rezzani20University of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaUniversity of BresciaBackground: In the development of hypertensive microvascular remodeling, a relevant role may be played by changes in extracellular matrix proteins. Aim of this study was the to evaluate some extracellular matrix components within the tunica media of subcutaneous small arteries in 9 normotensive subjects and 12 essential hypertensive patients, submitted to a biopsy of subcutaneous fat from the gluteal or the anterior abdominal region. Patients and Methods: Subcutaneous small resistance arteries were dissected and mounted on an isometric myograph, and the tunica media to internal lumen ratio was measured. In addition, fibronectin, laminin, transforming growth factor-beta-1 (TGF-β1) and emilin-1 contents within the tunica media were evaluated by immunofluorescence and relative immunomorphometrical analysis (immunopositivity % of area). The total collagen content and collagen subtypes within the tunica media were evaluated using both Sirius red staining (under polarized light) and immunofluorescence assay. Results: Normotensive controls had less total and type III collagen in respect with hypertensive patients. Fibronectin and TGF-β1 tunica media content was significantly greater in essential hypertensive patients, compared with normotensive controls, while laminin and emilin-1 tunica media content was lesser in essential hypertensive patients, compared with normotensive controls. A significant correlation was observed between fibronectin tunica media content and media to lumen ratio. Conclusions: Our results indicate that, in small resistance arteries of patients with essential hypertension, a relevant fibrosis may be detected; fibronectin and TGF-β1 tunica media content is increased, while laminin and emilin-1 content is decreased; these changes might be involved in the development of small resistance artery remodeling in humans.http://dx.doi.org/10.1080/08037051.2018.1448256microcirculationextracellular matrixcollagenhypertensionsmall resistance arteriesfibrosis |
| spellingShingle | Gaia Favero Anna Paini Carolina De Ciuceis Luigi F. Rodella Enrico Moretti Enzo Porteri Claudia Rossini Silvia Ministrini Leonardo Solaini Caletti Stefano Maria Antonietta Coschignano Valeria Brami Alina Petelca Matteo Nardin Ilenia Valli Guido A. M. Tiberio Francesca Bonomini Claudia Agabiti Rosei Nazario Portolani Damiano Rizzoni Rita Rezzani Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension Blood Pressure microcirculation extracellular matrix collagen hypertension small resistance arteries fibrosis |
| title | Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| title_full | Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| title_fullStr | Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| title_full_unstemmed | Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| title_short | Changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| title_sort | changes in extracellular matrix in subcutaneous small resistance arteries of patients with essential hypertension |
| topic | microcirculation extracellular matrix collagen hypertension small resistance arteries fibrosis |
| url | http://dx.doi.org/10.1080/08037051.2018.1448256 |
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