Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin
Administration of bovine somatotropin (bST) to lactating cows results in an increase in milk production from 10 to 15%. While physiological mechanisms involved in bST administration are well known, there is limited knowledge about the mechanisms that regulate the bST action at genetic level. For thi...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2010-01-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/303 |
| _version_ | 1818024818077859840 |
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| author | Mark A. McGuire Aldo Cappio-Borlino Ana H. Dias Francesconi Corrado Dimauro |
| author_facet | Mark A. McGuire Aldo Cappio-Borlino Ana H. Dias Francesconi Corrado Dimauro |
| author_sort | Mark A. McGuire |
| collection | DOAJ |
| description | Administration of bovine somatotropin (bST) to lactating cows results in an increase in milk production from 10 to 15%. While physiological mechanisms involved in bST administration are well known, there is limited knowledge about the mechanisms that regulate the bST action at genetic level. For this reason, a microarray experiment was conducted to identify differentially expressed genes when bST is given to milking cows. Sixteen high-density microarrays for cattle, each containing 18,263 gene spots, were used. RNA was extracted from the mammary tissue of four lactating Holstein cows, five and two days before, and one and six days after bST administration. A total of 1,251 and 1,167 differentially expressed genes were detected for mean and median expression intensities, respectively. Only the 115 genes which were identified by both mean and median intensities were taken into account. These genes were grouped into 8 clusters according to changes in expression through time points. |
| first_indexed | 2024-12-10T04:06:15Z |
| format | Article |
| id | doaj.art-a1dc9548d430456f9c303300cc194341 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-10T04:06:15Z |
| publishDate | 2010-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-a1dc9548d430456f9c303300cc1943412022-12-22T02:02:50ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2010-01-0182s788010.4081/ijas.2009.s2.78Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropinMark A. McGuireAldo Cappio-BorlinoAna H. Dias FrancesconiCorrado DimauroAdministration of bovine somatotropin (bST) to lactating cows results in an increase in milk production from 10 to 15%. While physiological mechanisms involved in bST administration are well known, there is limited knowledge about the mechanisms that regulate the bST action at genetic level. For this reason, a microarray experiment was conducted to identify differentially expressed genes when bST is given to milking cows. Sixteen high-density microarrays for cattle, each containing 18,263 gene spots, were used. RNA was extracted from the mammary tissue of four lactating Holstein cows, five and two days before, and one and six days after bST administration. A total of 1,251 and 1,167 differentially expressed genes were detected for mean and median expression intensities, respectively. Only the 115 genes which were identified by both mean and median intensities were taken into account. These genes were grouped into 8 clusters according to changes in expression through time points.http://www.aspajournal.it/index.php/ijas/article/view/303Microarray, Somatotropin, Cow. |
| spellingShingle | Mark A. McGuire Aldo Cappio-Borlino Ana H. Dias Francesconi Corrado Dimauro Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin Italian Journal of Animal Science Microarray, Somatotropin, Cow. |
| title | Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| title_full | Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| title_fullStr | Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| title_full_unstemmed | Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| title_short | Microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| title_sort | microarray data analysis of gene expression levels in lactating cows treated with bovine somatotropin |
| topic | Microarray, Somatotropin, Cow. |
| url | http://www.aspajournal.it/index.php/ijas/article/view/303 |
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