Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species
Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mito...
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MDPI AG
2022-07-01
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| author | Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti |
| author_facet | Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti |
| author_sort | Gunjan Katyal |
| collection | DOAJ |
| description | Antarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mitochondrial proteomes of both the red and white muscle of two different icefish species (<i>Champsocephalus gunnari</i> and <i>Chionodraco rastrospinosus)</i> and compared these with two related red-blooded Notothenioids (<i>Notothenia rossii</i>, <i>Trematomus bernacchii</i>). Liquid Chromatography-Mass spectrometry (LC-MS/MS) was used to generate and examine the proteomic profiles of the two groups. We recorded a total of 91 differentially expressed proteins in the icefish red muscle mitochondria and 89 in the white muscle mitochondria when compared with the red-blooded related species. The icefish have a relatively higher abundance of proteins involved with Complex V of oxidative phosphorylation, RNA metabolism, and homeostasis, and fewer proteins for striated muscle contraction, haem, iron, creatine, and carbohydrate metabolism. Enrichment analyses showed that many important pathways were different in both red muscle and white muscle, including the citric acid cycle, ribosome machinery and fatty acid degradation. Life in the Antarctic waters poses extra challenges to the organisms that reside within them. Icefish have successfully inhabited this environment and we surmise that species without haemoglobin uniquely maintain their physiology. Our study highlights the mitochondrial protein pathway differences between similar fish species according to their specific tissue oxygenation idiosyncrasies. |
| first_indexed | 2024-03-09T10:01:44Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T10:01:44Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj.art-a1de3df480794946b5c7a71dc5ae40472023-12-01T23:25:55ZengMDPI AGBiology2079-77372022-07-01118111810.3390/biology11081118Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded SpeciesGunjan Katyal0Brad Ebanks1Adam Dowle2Freya Shephard3Chiara Papetti4Magnus Lucassen5Lisa Chakrabarti6School of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UKSchool of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UKDepartment of Biology, Bioscience Technology Facility, University of York, York YO10 5DD, UKSchool of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UKBiology Department, University of Padova, Via U. Bassi, 58/b, 35121 Padova, ItalyAlfred Wegener Institute, 27570 Bremerhaven, GermanySchool of Veterinary Medicine and Science, University of Nottingham, Sutton Bonington LE12 5RD, UKAntarctic icefish are extraordinary in their ability to thrive without haemoglobin. We wanted to understand how the mitochondrial proteome has adapted to the loss of this protein. Metabolic pathways that utilise oxygen are most likely to be rearranged in these species. Here, we have defined the mitochondrial proteomes of both the red and white muscle of two different icefish species (<i>Champsocephalus gunnari</i> and <i>Chionodraco rastrospinosus)</i> and compared these with two related red-blooded Notothenioids (<i>Notothenia rossii</i>, <i>Trematomus bernacchii</i>). Liquid Chromatography-Mass spectrometry (LC-MS/MS) was used to generate and examine the proteomic profiles of the two groups. We recorded a total of 91 differentially expressed proteins in the icefish red muscle mitochondria and 89 in the white muscle mitochondria when compared with the red-blooded related species. The icefish have a relatively higher abundance of proteins involved with Complex V of oxidative phosphorylation, RNA metabolism, and homeostasis, and fewer proteins for striated muscle contraction, haem, iron, creatine, and carbohydrate metabolism. Enrichment analyses showed that many important pathways were different in both red muscle and white muscle, including the citric acid cycle, ribosome machinery and fatty acid degradation. Life in the Antarctic waters poses extra challenges to the organisms that reside within them. Icefish have successfully inhabited this environment and we surmise that species without haemoglobin uniquely maintain their physiology. Our study highlights the mitochondrial protein pathway differences between similar fish species according to their specific tissue oxygenation idiosyncrasies.https://www.mdpi.com/2079-7737/11/8/1118icefishproteomicsmitochondriamusclenetwork analysisnotothenioid |
| spellingShingle | Gunjan Katyal Brad Ebanks Adam Dowle Freya Shephard Chiara Papetti Magnus Lucassen Lisa Chakrabarti Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species Biology icefish proteomics mitochondria muscle network analysis notothenioid |
| title | Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
| title_full | Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
| title_fullStr | Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
| title_full_unstemmed | Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
| title_short | Quantitative Proteomics and Network Analysis of Differentially Expressed Proteins in Proteomes of Icefish Muscle Mitochondria Compared with Closely Related Red-Blooded Species |
| title_sort | quantitative proteomics and network analysis of differentially expressed proteins in proteomes of icefish muscle mitochondria compared with closely related red blooded species |
| topic | icefish proteomics mitochondria muscle network analysis notothenioid |
| url | https://www.mdpi.com/2079-7737/11/8/1118 |
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