Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China
Extended-spectrum beta-lactamase-producing Gram-negative bacteria are common in the community and hospitals. To monitor ESBLs mediated by the CTX-M genotype, we collected clinical ESBL pathogenic strains from a hospital in central China and observed a strain of Escherichia coli, namely Ec15103 carry...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158659/full |
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| author | Mingxing Yang Dong Liu Xiaoquan Li Chuting Xiao Yingge Mao Jiaqi He Jiao Feng Li Wang |
| author_facet | Mingxing Yang Dong Liu Xiaoquan Li Chuting Xiao Yingge Mao Jiaqi He Jiao Feng Li Wang |
| author_sort | Mingxing Yang |
| collection | DOAJ |
| description | Extended-spectrum beta-lactamase-producing Gram-negative bacteria are common in the community and hospitals. To monitor ESBLs mediated by the CTX-M genotype, we collected clinical ESBL pathogenic strains from a hospital in central China and observed a strain of Escherichia coli, namely Ec15103 carrying blaCTX–M–14, blaCTX–M–64 and blaTEM–1, isolated from the blood of a 7-day-old infant in 2015. Strain Ec15103 contains two drug resistance plasmids: pEc15103A, an IncFI-type plasmid that cannot be conjugatively transferred and carries the drug resistance genes blaTEM–1, aacC2, aadA5, sul1, mph(A), sul2, strAB, and tetA(A); and pEc15103B, an IncK2/Z-type plasmid that carries the conjugation transfer gene and blaCTX–M–14. In addition, blaCTX–M–64 is located on the chromosome of Ec15103, and it is the first report of pathogen with blaCTX–M–64 located on its chromosome (the search terms used “blaCTX-M-64” and “chromosome”). blaCTX–M–14 and blaCTX–M–64 are carried by ISEcp1-mediated transposon Tn6503a and Tn6502, respectively. The conjugation transfer ability of pEc15103B was significantly inhibited by zidovudine (AZT) and linoleic acid (LA) and that expression of blaCTX–M–14, blaCTX–M–64 and blaTEM–1 at the mRNA level did not change based on the concentration of cefotaxime or ampicillin. Co-occurrence of blaCTX–M–14 and blaCTX–M–64 in a single isolate will enhance the drug resistance of bacteria, and the presence of blaCTX–M–64 in the chromosome may make the resistance more maintain. This fact will facilitate its dissemination and persistence under different antimicrobial selection pressures. It is essential to prevent these strains from further spreading in a hospital environment. |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-03-12T11:51:46Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-a1e1b72ac1474496b6c3a14a7282dd3a2023-08-31T07:38:38ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-05-011410.3389/fmicb.2023.11586591158659Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from ChinaMingxing Yang0Dong Liu1Xiaoquan Li2Chuting Xiao3Yingge Mao4Jiaqi He5Jiao Feng6Li Wang7Department of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaInstitute of Biomedical Sciences, The Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China, The Key Laboratory of Medical Molecular Cell Biology of Shanxi Province, Shanxi University, Taiyuan, ChinaDepartment of Clinical Laboratory, The First Affiliated Hospital of Henan University, Kaifeng, ChinaExtended-spectrum beta-lactamase-producing Gram-negative bacteria are common in the community and hospitals. To monitor ESBLs mediated by the CTX-M genotype, we collected clinical ESBL pathogenic strains from a hospital in central China and observed a strain of Escherichia coli, namely Ec15103 carrying blaCTX–M–14, blaCTX–M–64 and blaTEM–1, isolated from the blood of a 7-day-old infant in 2015. Strain Ec15103 contains two drug resistance plasmids: pEc15103A, an IncFI-type plasmid that cannot be conjugatively transferred and carries the drug resistance genes blaTEM–1, aacC2, aadA5, sul1, mph(A), sul2, strAB, and tetA(A); and pEc15103B, an IncK2/Z-type plasmid that carries the conjugation transfer gene and blaCTX–M–14. In addition, blaCTX–M–64 is located on the chromosome of Ec15103, and it is the first report of pathogen with blaCTX–M–64 located on its chromosome (the search terms used “blaCTX-M-64” and “chromosome”). blaCTX–M–14 and blaCTX–M–64 are carried by ISEcp1-mediated transposon Tn6503a and Tn6502, respectively. The conjugation transfer ability of pEc15103B was significantly inhibited by zidovudine (AZT) and linoleic acid (LA) and that expression of blaCTX–M–14, blaCTX–M–64 and blaTEM–1 at the mRNA level did not change based on the concentration of cefotaxime or ampicillin. Co-occurrence of blaCTX–M–14 and blaCTX–M–64 in a single isolate will enhance the drug resistance of bacteria, and the presence of blaCTX–M–64 in the chromosome may make the resistance more maintain. This fact will facilitate its dissemination and persistence under different antimicrobial selection pressures. It is essential to prevent these strains from further spreading in a hospital environment.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158659/fullEscherichia coliantimicrobial resistanceESBLsCTX-M-14CTX-M-64 |
| spellingShingle | Mingxing Yang Dong Liu Xiaoquan Li Chuting Xiao Yingge Mao Jiaqi He Jiao Feng Li Wang Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China Frontiers in Microbiology Escherichia coli antimicrobial resistance ESBLs CTX-M-14 CTX-M-64 |
| title | Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China |
| title_full | Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China |
| title_fullStr | Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China |
| title_full_unstemmed | Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China |
| title_short | Characterizations of blaCTX–M–14 and blaCTX–M–64 in a clinical isolate of Escherichia coli from China |
| title_sort | characterizations of blactx m 14 and blactx m 64 in a clinical isolate of escherichia coli from china |
| topic | Escherichia coli antimicrobial resistance ESBLs CTX-M-14 CTX-M-64 |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1158659/full |
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