Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017
Since 2017, the new H7N9 highly pathogenic avian influenza viruses (HPAIVs) have been responsible for more than 200,000 cases of chicken infection and more than 120,000 chicken deaths in China. Our previous study found that the Q26 was chicken-origin H7N9 HPAIV. In this study, we analyzed the geneti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1105529/full |
| _version_ | 1827997736426799104 |
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| author | Bingbing Zhao Bingbing Zhao Bingbing Zhao Wenqing Wang Yating Song Xiangyang Wen Siyu Feng Weiqiang Li Yangbao Ding Zuxian Chen Zhuoliang He Shao Wang Peirong Jiao Peirong Jiao |
| author_facet | Bingbing Zhao Bingbing Zhao Bingbing Zhao Wenqing Wang Yating Song Xiangyang Wen Siyu Feng Weiqiang Li Yangbao Ding Zuxian Chen Zhuoliang He Shao Wang Peirong Jiao Peirong Jiao |
| author_sort | Bingbing Zhao |
| collection | DOAJ |
| description | Since 2017, the new H7N9 highly pathogenic avian influenza viruses (HPAIVs) have been responsible for more than 200,000 cases of chicken infection and more than 120,000 chicken deaths in China. Our previous study found that the Q26 was chicken-origin H7N9 HPAIV. In this study, we analyzed the genetic characterization of Q24, Q65, Q66, Q85, and Q102 H7N9 avian influenza viruses isolated from Guangdong, China in 2017. Our results showed that these viruses were highly pathogenic and belonged to two different genotypes, which suggested they occurred genetic reassortant. To investigate the pathogenicity, transmission, and host immune responses of H7N9 virus in chickens, we selected Q24 and Q26 viruses to inoculate chickens. The Q24 and Q26 viruses killed all inoculated chickens within 3 days and replicated effectively in all tested tissues. They were efficiently transmitted to contact chickens and killed them within 4 days through direct contact. Furthermore, we found that the expressions of several immune-related genes (e.g., TLR3, TLR7, MDA5, MAVS, IFN-β, IL-6, IL-8, OAS, Mx1, MHC I, and MHC II) were upregulated obviously in the lungs and spleen of chickens inoculated with the two H7N9 viruses at 24 h post-inoculation (HPI). Among these, IL-6 and IFN-β in lungs were the most upregulated (by 341.02–381.48-fold and 472.50–500.56-fold, respectively). These results suggest that the new H7N9 viruses isolated in 2017, can replicate and transmit effectively and trigger strong immune responses in chickens, which helps us understand the genetic and pathogenic variations of H7N9 HPAIVs in China. |
| first_indexed | 2024-04-10T05:33:41Z |
| format | Article |
| id | doaj.art-a1e82a80b2284523b150c9884ea43397 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-10T05:33:41Z |
| publishDate | 2023-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a1e82a80b2284523b150c9884ea433972023-03-07T05:22:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2023-03-011410.3389/fmicb.2023.11055291105529Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017Bingbing Zhao0Bingbing Zhao1Bingbing Zhao2Wenqing Wang3Yating Song4Xiangyang Wen5Siyu Feng6Weiqiang Li7Yangbao Ding8Zuxian Chen9Zhuoliang He10Shao Wang11Peirong Jiao12Peirong Jiao13Guangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, ChinaAnimal Influenza Laboratory of the Ministry of Agriculture and Rural Affairs, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fuzhou, ChinaGuangdong Laboratory for Lingnan Modern Agriculture, College of Veterinary Medicine, South China Agricultural University, Guangzhou, ChinaGuangdong Provincial Key Laboratory of Zoonosis Prevention and Control, Guangzhou, ChinaSince 2017, the new H7N9 highly pathogenic avian influenza viruses (HPAIVs) have been responsible for more than 200,000 cases of chicken infection and more than 120,000 chicken deaths in China. Our previous study found that the Q26 was chicken-origin H7N9 HPAIV. In this study, we analyzed the genetic characterization of Q24, Q65, Q66, Q85, and Q102 H7N9 avian influenza viruses isolated from Guangdong, China in 2017. Our results showed that these viruses were highly pathogenic and belonged to two different genotypes, which suggested they occurred genetic reassortant. To investigate the pathogenicity, transmission, and host immune responses of H7N9 virus in chickens, we selected Q24 and Q26 viruses to inoculate chickens. The Q24 and Q26 viruses killed all inoculated chickens within 3 days and replicated effectively in all tested tissues. They were efficiently transmitted to contact chickens and killed them within 4 days through direct contact. Furthermore, we found that the expressions of several immune-related genes (e.g., TLR3, TLR7, MDA5, MAVS, IFN-β, IL-6, IL-8, OAS, Mx1, MHC I, and MHC II) were upregulated obviously in the lungs and spleen of chickens inoculated with the two H7N9 viruses at 24 h post-inoculation (HPI). Among these, IL-6 and IFN-β in lungs were the most upregulated (by 341.02–381.48-fold and 472.50–500.56-fold, respectively). These results suggest that the new H7N9 viruses isolated in 2017, can replicate and transmit effectively and trigger strong immune responses in chickens, which helps us understand the genetic and pathogenic variations of H7N9 HPAIVs in China.https://www.frontiersin.org/articles/10.3389/fmicb.2023.1105529/fullH7N9 highly pathogenic avian influenza virusgenetic characterizationpathogenicitytransmissionimmune responseschickens |
| spellingShingle | Bingbing Zhao Bingbing Zhao Bingbing Zhao Wenqing Wang Yating Song Xiangyang Wen Siyu Feng Weiqiang Li Yangbao Ding Zuxian Chen Zhuoliang He Shao Wang Peirong Jiao Peirong Jiao Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 Frontiers in Microbiology H7N9 highly pathogenic avian influenza virus genetic characterization pathogenicity transmission immune responses chickens |
| title | Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 |
| title_full | Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 |
| title_fullStr | Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 |
| title_full_unstemmed | Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 |
| title_short | Genetic characterization and pathogenicity of H7N9 highly pathogenic avian influenza viruses isolated from South China in 2017 |
| title_sort | genetic characterization and pathogenicity of h7n9 highly pathogenic avian influenza viruses isolated from south china in 2017 |
| topic | H7N9 highly pathogenic avian influenza virus genetic characterization pathogenicity transmission immune responses chickens |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2023.1105529/full |
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