Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus
Abstract Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at the fine scale in the mammalian brain. To address this question, we functionally mapped the spatial organization of the input a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-023-43218-x |
| _version_ | 1827709912565678080 |
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| author | Dmitry Molotkov Leiron Ferrarese Tom Boissonnet Hiroki Asari |
| author_facet | Dmitry Molotkov Leiron Ferrarese Tom Boissonnet Hiroki Asari |
| author_sort | Dmitry Molotkov |
| collection | DOAJ |
| description | Abstract Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at the fine scale in the mammalian brain. To address this question, we functionally mapped the spatial organization of the input axons and target neurons in the female mouse retinocollicular pathway at single-cell resolution using in vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling of retinal ganglion cell axon terminals, with an average error below 30 μm or 2° of visual angle. The precision of retinotopy was relatively lower for local neurons in the superior colliculus. Subsequent data-driven modeling ascribed it to a low input convergence, on average 5.5 retinal ganglion cell inputs per postsynaptic cell in the superior colliculus. These results indicate that retinotopy arises largely from topographically precise input from presynaptic cells, rather than elaborating local circuitry to reconstruct the topography by postsynaptic cells. |
| first_indexed | 2024-03-10T17:29:55Z |
| format | Article |
| id | doaj.art-a1eb8bb23dd44618833d566c57e62775 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-03-10T17:29:55Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-a1eb8bb23dd44618833d566c57e627752023-11-20T10:04:09ZengNature PortfolioNature Communications2041-17232023-11-0114111010.1038/s41467-023-43218-xTopographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculusDmitry Molotkov0Leiron Ferrarese1Tom Boissonnet2Hiroki Asari3Epigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology LaboratoryEpigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology LaboratoryEpigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology LaboratoryEpigenetics and Neurobiology Unit, EMBL Rome, European Molecular Biology LaboratoryAbstract Retinotopy, like all long-range projections, can arise from the axons themselves or their targets. The underlying connectivity pattern, however, remains elusive at the fine scale in the mammalian brain. To address this question, we functionally mapped the spatial organization of the input axons and target neurons in the female mouse retinocollicular pathway at single-cell resolution using in vivo two-photon calcium imaging. We found a near-perfect retinotopic tiling of retinal ganglion cell axon terminals, with an average error below 30 μm or 2° of visual angle. The precision of retinotopy was relatively lower for local neurons in the superior colliculus. Subsequent data-driven modeling ascribed it to a low input convergence, on average 5.5 retinal ganglion cell inputs per postsynaptic cell in the superior colliculus. These results indicate that retinotopy arises largely from topographically precise input from presynaptic cells, rather than elaborating local circuitry to reconstruct the topography by postsynaptic cells.https://doi.org/10.1038/s41467-023-43218-x |
| spellingShingle | Dmitry Molotkov Leiron Ferrarese Tom Boissonnet Hiroki Asari Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus Nature Communications |
| title | Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus |
| title_full | Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus |
| title_fullStr | Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus |
| title_full_unstemmed | Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus |
| title_short | Topographic axonal projection at single-cell precision supports local retinotopy in the mouse superior colliculus |
| title_sort | topographic axonal projection at single cell precision supports local retinotopy in the mouse superior colliculus |
| url | https://doi.org/10.1038/s41467-023-43218-x |
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