Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke
The burden of stroke is high and is continually increasing due to a dramatic growth in the world's elderly population. Novel therapeutic strategies are therefore required. The present study sought to determine the effect of laser acupuncture at GV20 on brain damage, oxidative-status markers in...
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| Format: | Article |
| Language: | English |
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Medical Association of Pharmacopuncture Institute
2017-10-01
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| Series: | Journal of Acupuncture & Meridian Studies |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2005290117300134 |
| _version_ | 1818921362301911040 |
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| author | Jinatta Jittiwat |
| author_facet | Jinatta Jittiwat |
| author_sort | Jinatta Jittiwat |
| collection | DOAJ |
| description | The burden of stroke is high and is continually increasing due to a dramatic growth in the world's elderly population. Novel therapeutic strategies are therefore required. The present study sought to determine the effect of laser acupuncture at GV20 on brain damage, oxidative-status markers in the cerebral cortex, and superoxide dismutase in the mitochondria of an animal model of focal ischemic stroke. Wistar rats, weighing 300–350 g, were divided into the following four groups: (1) control; (2) permanent occlusion of the right middle cerebral artery (Rt.MCAO) alone; (3) Rt.MCAO plus sham laser acupuncture; and (4) Rt.MCAO plus laser-acupuncture groups. Sham laser acupuncture or laser acupuncture was performed once daily at the GV20 (Baihui) acupoint for 14 days following Rt.MCAO. Half of the rats in each group were examined by 2,3,5-triphenyltetrazolium chloride staining to determine the brain infarct volume, while the other half were examined by biochemical assays to determine the malondialdehyde level, and the glutathione peroxidase, catalase, and superoxide-dismutase activities in the brain-cortex mitochondria. The results showed that laser acupuncture at GV20 significantly decreased the brain infarct volume and malondialdehyde level, and increased the catalase, glutathione peroxidase, and superoxide-dismutase activities in cerebral ischemic rats. In conclusion, laser acupuncture at GV20 decreases the brain infarct volume in cerebral ischemic rats, at least in part due to decreased oxidative stress. Further study is warranted to investigate other possible underlying mechanisms. |
| first_indexed | 2024-12-20T01:36:26Z |
| format | Article |
| id | doaj.art-a1f5806434074eabb45cf739671e80cd |
| institution | Directory Open Access Journal |
| issn | 2005-2901 |
| language | English |
| last_indexed | 2024-12-20T01:36:26Z |
| publishDate | 2017-10-01 |
| publisher | Medical Association of Pharmacopuncture Institute |
| record_format | Article |
| series | Journal of Acupuncture & Meridian Studies |
| spelling | doaj.art-a1f5806434074eabb45cf739671e80cd2022-12-21T19:58:00ZengMedical Association of Pharmacopuncture InstituteJournal of Acupuncture & Meridian Studies2005-29012017-10-0110532433010.1016/j.jams.2017.08.003Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic StrokeJinatta Jittiwat0Faculty of Medicine, Mahasarakham University, Mahasarakham, ThailandThe burden of stroke is high and is continually increasing due to a dramatic growth in the world's elderly population. Novel therapeutic strategies are therefore required. The present study sought to determine the effect of laser acupuncture at GV20 on brain damage, oxidative-status markers in the cerebral cortex, and superoxide dismutase in the mitochondria of an animal model of focal ischemic stroke. Wistar rats, weighing 300–350 g, were divided into the following four groups: (1) control; (2) permanent occlusion of the right middle cerebral artery (Rt.MCAO) alone; (3) Rt.MCAO plus sham laser acupuncture; and (4) Rt.MCAO plus laser-acupuncture groups. Sham laser acupuncture or laser acupuncture was performed once daily at the GV20 (Baihui) acupoint for 14 days following Rt.MCAO. Half of the rats in each group were examined by 2,3,5-triphenyltetrazolium chloride staining to determine the brain infarct volume, while the other half were examined by biochemical assays to determine the malondialdehyde level, and the glutathione peroxidase, catalase, and superoxide-dismutase activities in the brain-cortex mitochondria. The results showed that laser acupuncture at GV20 significantly decreased the brain infarct volume and malondialdehyde level, and increased the catalase, glutathione peroxidase, and superoxide-dismutase activities in cerebral ischemic rats. In conclusion, laser acupuncture at GV20 decreases the brain infarct volume in cerebral ischemic rats, at least in part due to decreased oxidative stress. Further study is warranted to investigate other possible underlying mechanisms.http://www.sciencedirect.com/science/article/pii/S2005290117300134baihui acupointfocal ischemic strokelaser acupunctureoxidative stress |
| spellingShingle | Jinatta Jittiwat Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke Journal of Acupuncture & Meridian Studies baihui acupoint focal ischemic stroke laser acupuncture oxidative stress |
| title | Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke |
| title_full | Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke |
| title_fullStr | Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke |
| title_full_unstemmed | Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke |
| title_short | Laser Acupuncture at GV20 Improves Brain Damage and Oxidative Stress in Animal Model of Focal Ischemic Stroke |
| title_sort | laser acupuncture at gv20 improves brain damage and oxidative stress in animal model of focal ischemic stroke |
| topic | baihui acupoint focal ischemic stroke laser acupuncture oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2005290117300134 |
| work_keys_str_mv | AT jinattajittiwat laseracupunctureatgv20improvesbraindamageandoxidativestressinanimalmodeloffocalischemicstroke |