Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation
It has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procure...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2002-07-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.3727/000000002783985602 |
| _version_ | 1819111742383325184 |
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| author | Nanae Takahashi Shin Enosawa Tasuku Mitani Hua Lu Seiichi Suzuki Hiroshi Amemiya Takashi Amano Norio Sakuragawa |
| author_facet | Nanae Takahashi Shin Enosawa Tasuku Mitani Hua Lu Seiichi Suzuki Hiroshi Amemiya Takashi Amano Norio Sakuragawa |
| author_sort | Nanae Takahashi |
| collection | DOAJ |
| description | It has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procurement. But transplantation of a sufficient number of cells to adult tissue needs large-scale cell supply and may lead to vascular embolism. We attempted transplantation of amniotic epithelial cells into fetal liver, because 1) the fetal liver is at the proliferative stage, 2) the number of cells required is small, and 3) the fetal stage is advantageous for the induction of immunological tolerance. Amniotic epithelial cells from day 18.5–20.5 fetuses were transfected with adenoviral AdlacZ and harvested to inject into fetal rat liver of the syngeneic strain (day 18.5–20.5). The efficacy of cell transplantation into the liver increased in the order: intraplacental < intraumbilical vein < intrahepatic route. LacZ-transfected amniotic cells (1–8 × 105 cells), hepatocytes (5 × 105 cells), or AdlacZ vector solution (1.7 × 107 pfu) were injected through the uterine membrane into the liver. Transplanted cells formed a cellular mass and survived for up to 14 days after birth, whereas lacZ-transfected cells were rapidly decreased after the injection of AdlacZ vector or rat hepatocytes as a gene carrier so that the use of amniotic epithelial cells as a gene carrier will result in long-term expression of exogenous genes in the liver. |
| first_indexed | 2024-12-22T04:02:27Z |
| format | Article |
| id | doaj.art-a1fa5bbd58f34cd3a2f1253bb7d8cb97 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-22T04:02:27Z |
| publishDate | 2002-07-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-a1fa5bbd58f34cd3a2f1253bb7d8cb972022-12-21T18:39:42ZengSAGE PublishingCell Transplantation0963-68971555-38922002-07-011110.3727/000000002783985602Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero ManipulationNanae Takahashi0Shin Enosawa1Tasuku Mitani2Hua Lu3Seiichi Suzuki4Hiroshi Amemiya5Takashi Amano6Norio Sakuragawa7National Research Institute for Child Health and DevelopmentNational Research Institute for Child Health and DevelopmentNational Research Institute for Child Health and DevelopmentNational Research Institute for Child Health and DevelopmentNational Research Institute for Child Health and DevelopmentNational Research Institute for Child Health and DevelopmentTokyo University of AgricultureNational Institute of NeuroscienceIt has been hoped that amniotic epithelial cells would be a gene carrier to neural and hepatic tissue, because of 1) the presence of neural and hepatic stem-like cells, 2) the ability to cryopreserve them, 3) long-term survival in the transplanted site, and 4) few ethical problems concerning procurement. But transplantation of a sufficient number of cells to adult tissue needs large-scale cell supply and may lead to vascular embolism. We attempted transplantation of amniotic epithelial cells into fetal liver, because 1) the fetal liver is at the proliferative stage, 2) the number of cells required is small, and 3) the fetal stage is advantageous for the induction of immunological tolerance. Amniotic epithelial cells from day 18.5–20.5 fetuses were transfected with adenoviral AdlacZ and harvested to inject into fetal rat liver of the syngeneic strain (day 18.5–20.5). The efficacy of cell transplantation into the liver increased in the order: intraplacental < intraumbilical vein < intrahepatic route. LacZ-transfected amniotic cells (1–8 × 105 cells), hepatocytes (5 × 105 cells), or AdlacZ vector solution (1.7 × 107 pfu) were injected through the uterine membrane into the liver. Transplanted cells formed a cellular mass and survived for up to 14 days after birth, whereas lacZ-transfected cells were rapidly decreased after the injection of AdlacZ vector or rat hepatocytes as a gene carrier so that the use of amniotic epithelial cells as a gene carrier will result in long-term expression of exogenous genes in the liver.https://doi.org/10.3727/000000002783985602 |
| spellingShingle | Nanae Takahashi Shin Enosawa Tasuku Mitani Hua Lu Seiichi Suzuki Hiroshi Amemiya Takashi Amano Norio Sakuragawa Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation Cell Transplantation |
| title | Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation |
| title_full | Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation |
| title_fullStr | Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation |
| title_full_unstemmed | Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation |
| title_short | Transplantation of Amniotic Epithelial Cells into Fetal Rat Liver by In Utero Manipulation |
| title_sort | transplantation of amniotic epithelial cells into fetal rat liver by in utero manipulation |
| url | https://doi.org/10.3727/000000002783985602 |
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