Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis
WRKY transcription factors play critical roles in the physiological processes of plants. Although the roles of WRKYs have been characterized in some model plants, their roles in woody plants, especially wintersweet (<i>Chimonanthus praecox</i>), are largely unclear. In this study, a wint...
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MDPI AG
2021-12-01
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| Series: | Genes |
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| author | Renwei Huang Shunzhao Sui Huamin Liu Mingyang Li Daofeng Liu |
| author_facet | Renwei Huang Shunzhao Sui Huamin Liu Mingyang Li Daofeng Liu |
| author_sort | Renwei Huang |
| collection | DOAJ |
| description | WRKY transcription factors play critical roles in the physiological processes of plants. Although the roles of WRKYs have been characterized in some model plants, their roles in woody plants, especially wintersweet (<i>Chimonanthus praecox</i>), are largely unclear. In this study, a wintersweet WRKY gene named <i>CpWRKY75</i> belonging to group IIc was isolated and its characteristics were identified. CpWRKY75 is a nucleus-localized protein, and exhibited no transcriptional activation activity in yeast. <i>CpWRKY75</i> was highly expressed in flowers at different bloom stages. Ectopic expression of <i>CpWRKY75</i> significantly promoted the flowering time of transgenic Arabidopsis (<i>Arabidopsis thaliana</i>), as determined by the rosette leaf number and first flower open time. The expression levels of flowering-related genes were quantified by qRT-PCR, and the results suggested that <i>CpWRKY75</i> had obvious influence on the expression level of <i>MICRORNA156C</i> (<i>MIR156C</i>), <i>SQUAMOSA PROMOTER BINDING PROTEIN-LIKE</i><i>3</i> (<i>SPL3</i>) and <i>SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9</i> (<i>SPL9</i>), <i>FLOWERING LOCUS T</i> (<i>FT</i>), <i>LEAFY</i> (<i>LFY</i>), <i>SUPPRESSOR OF OVEREXPRESSION OF CO 1</i> (<i>SOC1</i>), <i>APETALA1</i> (<i>AP1</i>), <i>CAULIFLOWER</i> (<i>CAL</i>), and <i>FRUITFULL</i> (<i>FUL</i>). These results suggest that <i>CpWRKY75</i> might have a flowering time regulation function, and additionally provide a new gene resource for the genetic engineering of woody flowering plants. |
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| language | English |
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| spelling | doaj.art-a1faab69ebca4955aff037d8be06dc162023-11-23T13:51:50ZengMDPI AGGenes2073-44252021-12-011316810.3390/genes13010068Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic ArabidopsisRenwei Huang0Shunzhao Sui1Huamin Liu2Mingyang Li3Daofeng Liu4Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources, College of Chemistry and Life Sciences, Chengdu Normal University, Chengdu 611130, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaCollege of Landscape Architecture and Life Science, Chongqing University of Arts and Sciences, Chongqing 402160, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaChongqing Engineering Research Center for Floriculture, Key Laboratory of Horticulture Science for Southern Mountainous Regions of Ministry of Education, College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, ChinaWRKY transcription factors play critical roles in the physiological processes of plants. Although the roles of WRKYs have been characterized in some model plants, their roles in woody plants, especially wintersweet (<i>Chimonanthus praecox</i>), are largely unclear. In this study, a wintersweet WRKY gene named <i>CpWRKY75</i> belonging to group IIc was isolated and its characteristics were identified. CpWRKY75 is a nucleus-localized protein, and exhibited no transcriptional activation activity in yeast. <i>CpWRKY75</i> was highly expressed in flowers at different bloom stages. Ectopic expression of <i>CpWRKY75</i> significantly promoted the flowering time of transgenic Arabidopsis (<i>Arabidopsis thaliana</i>), as determined by the rosette leaf number and first flower open time. The expression levels of flowering-related genes were quantified by qRT-PCR, and the results suggested that <i>CpWRKY75</i> had obvious influence on the expression level of <i>MICRORNA156C</i> (<i>MIR156C</i>), <i>SQUAMOSA PROMOTER BINDING PROTEIN-LIKE</i><i>3</i> (<i>SPL3</i>) and <i>SQUAMOSA PROMOTER BINDING PROTEIN-LIKE 9</i> (<i>SPL9</i>), <i>FLOWERING LOCUS T</i> (<i>FT</i>), <i>LEAFY</i> (<i>LFY</i>), <i>SUPPRESSOR OF OVEREXPRESSION OF CO 1</i> (<i>SOC1</i>), <i>APETALA1</i> (<i>AP1</i>), <i>CAULIFLOWER</i> (<i>CAL</i>), and <i>FRUITFULL</i> (<i>FUL</i>). These results suggest that <i>CpWRKY75</i> might have a flowering time regulation function, and additionally provide a new gene resource for the genetic engineering of woody flowering plants.https://www.mdpi.com/2073-4425/13/1/68wintersweet<i>CpWRKY75</i>expression patternflowering |
| spellingShingle | Renwei Huang Shunzhao Sui Huamin Liu Mingyang Li Daofeng Liu Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis Genes wintersweet <i>CpWRKY75</i> expression pattern flowering |
| title | Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis |
| title_full | Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis |
| title_fullStr | Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis |
| title_full_unstemmed | Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis |
| title_short | Overexpression of <i>CpWRKY75</i> from <i>Chimonanthus praecox</i> Promotes Flowering Time in Transgenic Arabidopsis |
| title_sort | overexpression of i cpwrky75 i from i chimonanthus praecox i promotes flowering time in transgenic arabidopsis |
| topic | wintersweet <i>CpWRKY75</i> expression pattern flowering |
| url | https://www.mdpi.com/2073-4425/13/1/68 |
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